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Article

Effects of unfractionated heparin and rivaroxaban on the expression of heparanase and fibroblast growth factor 2 in human osteoblasts

  • Authors:
    • Jingjun Xia
    • Wei Sheng
    • Liubao Pei
    • Ning Li
    • Zhipeng Zhang
    • Jialiang Wang
    • Jianing Zu
    • Nanxiang Wang
    • Duanyang Wang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China, Department of Neurology, The Second Hospital of Harbin, Harbin, Heilongjiang 150001, P.R. China, Department of Orthopaedics, Shuangyashan Coal General Hospital, Shuangyashan, Heilongjiang 155100, P.R. China, Department of Orthopaedics, The Fifth Hospital of Harbin, Harbin, Heilongjiang 150001, P.R. China
  • Pages: 361-366
    |
    Published online on: May 10, 2017
       https://doi.org/10.3892/mmr.2017.6570
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Abstract

Long-term treatment with anticoagulants may contribute to osteoporosis. Although unfractionated heparin and rivaroxaban have adverse effects on bone microstructure and function in adult rats, the underlying mechanism remains to be elucidated. Heparanase (HPSE) and fibroblast growth factor (FGF)2 are important signals in bone formation and fracture healing. Therefore, the present study was designed to investigate the effects of unfractionated heparin and rivaroxaban on the expression of HPSE and FGF2 in human osteoblasts. Human osteoblasts were treated with unfractionated heparin (0.5-50 IU/ml) or rivaroxaban (0.13‑13 µg/ml) for different durations. Plasmids encoding HPSE and FGF2 were transfected into osteoblasts, and cell viability was assessed using MTT assays, with mRNA and protein expression levels determined using reverse transcription‑quantitative polymerase chain reaction and western blot analyses, respectively. Osteoblast growth was significantly inhibited by treatment with unfractionated heparin (50 IU/ml) or rivaroxaban (13 µg/ml). Unfractionated heparin alone significantly inhibited the expression of HPSE and FGF2, whereas rivaroxaban inhibited the expression of FGF2 without affecting that of HPSE. Furthermore, the overexpression of HPSE or FGF2 significantly reversed the inhibitory effects of unfractionated heparin and rivaroxaban on osteoblasts. These findings suggested that HPSE and FGF2 signals were involved in the detrimental role of unfractionated heparin and rivaroxaban in human osteoblasts, providing novel information on the side effects of anticoagulants.
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Copy and paste a formatted citation
Spandidos Publications style
Xia J, Sheng W, Pei L, Li N, Zhang Z, Wang J, Zu J, Wang N and Wang D: Effects of unfractionated heparin and rivaroxaban on the expression of heparanase and fibroblast growth factor 2 in human osteoblasts. Mol Med Rep 16: 361-366, 2017.
APA
Xia, J., Sheng, W., Pei, L., Li, N., Zhang, Z., Wang, J. ... Wang, D. (2017). Effects of unfractionated heparin and rivaroxaban on the expression of heparanase and fibroblast growth factor 2 in human osteoblasts. Molecular Medicine Reports, 16, 361-366. https://doi.org/10.3892/mmr.2017.6570
MLA
Xia, J., Sheng, W., Pei, L., Li, N., Zhang, Z., Wang, J., Zu, J., Wang, N., Wang, D."Effects of unfractionated heparin and rivaroxaban on the expression of heparanase and fibroblast growth factor 2 in human osteoblasts". Molecular Medicine Reports 16.1 (2017): 361-366.
Chicago
Xia, J., Sheng, W., Pei, L., Li, N., Zhang, Z., Wang, J., Zu, J., Wang, N., Wang, D."Effects of unfractionated heparin and rivaroxaban on the expression of heparanase and fibroblast growth factor 2 in human osteoblasts". Molecular Medicine Reports 16, no. 1 (2017): 361-366. https://doi.org/10.3892/mmr.2017.6570
Copy and paste a formatted citation
x
Spandidos Publications style
Xia J, Sheng W, Pei L, Li N, Zhang Z, Wang J, Zu J, Wang N and Wang D: Effects of unfractionated heparin and rivaroxaban on the expression of heparanase and fibroblast growth factor 2 in human osteoblasts. Mol Med Rep 16: 361-366, 2017.
APA
Xia, J., Sheng, W., Pei, L., Li, N., Zhang, Z., Wang, J. ... Wang, D. (2017). Effects of unfractionated heparin and rivaroxaban on the expression of heparanase and fibroblast growth factor 2 in human osteoblasts. Molecular Medicine Reports, 16, 361-366. https://doi.org/10.3892/mmr.2017.6570
MLA
Xia, J., Sheng, W., Pei, L., Li, N., Zhang, Z., Wang, J., Zu, J., Wang, N., Wang, D."Effects of unfractionated heparin and rivaroxaban on the expression of heparanase and fibroblast growth factor 2 in human osteoblasts". Molecular Medicine Reports 16.1 (2017): 361-366.
Chicago
Xia, J., Sheng, W., Pei, L., Li, N., Zhang, Z., Wang, J., Zu, J., Wang, N., Wang, D."Effects of unfractionated heparin and rivaroxaban on the expression of heparanase and fibroblast growth factor 2 in human osteoblasts". Molecular Medicine Reports 16, no. 1 (2017): 361-366. https://doi.org/10.3892/mmr.2017.6570
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