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Article

Synergistic protective effect of FTY720 and vitamin E against simulated cerebral ischemia in vitro

  • Authors:
    • Xin Pang
    • Xuening Hou
  • View Affiliations / Copyright

    Affiliations: Key Laboratory for Corneal Tissue Engineering, College of Marine Life Sciences, Ocean University of China, Qingdao, Shandong 266003, P.R. China
  • Pages: 396-402
    |
    Published online on: May 11, 2017
       https://doi.org/10.3892/mmr.2017.6572
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Abstract

The purpose of the present study was to explore the combination effect of FTY720 and vitamin E on cerebral ischemia. Astrocytes were isolated from newborn Sprague‑Dawley rats and were subjected to FTY720, vitamin E, or combination of the two. The astrocyte cultures were then exposed to oxygen‑glucose deprivation (OGD) to simulate an ischemic model in vitro. Cell viability, lactate dehydrogenase (LDH) leakage and cell apoptosis were detected following 12 h of exposure to OGD. In addition, the levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑6, IL‑1β, total antioxidant capacity, intercellular adhesion molecule (ICAM)‑1, vascular cell adhesion molecule (VCAM)‑1, chemokine (C‑X‑C motif) ligand (CXCL)‑10, heme oxygenase (HO)‑1 and superoxide dismutase (SOD)‑1 were measured. Pre‑treatment with FTY720 or vitamin E significantly elevated the cell viability and decreased LDH release and number of apoptotic cells. Combination treatment with FTY720 and vitamin E demonstrated a synergistic protective effect on OGD‑induced cell viability, toxicity and apoptosis. Pre‑treatment with FTY720 markedly reduced the release of IL‑1β, TNF‑α, IL‑6, ICAM‑1, VCAM‑1 and CXCL‑10, and pre‑treatment with vitamin E increased the levels of antioxidant, HO‑1 and SOD‑1. However, pre‑treatment with FTY720 combined with vitamin E revealed a synergistic effect. Pre‑treatment with FTY720 combined with vitamin E exerts synergistic neuroprotective effects in the simulated cerebral ischemia in vitro.
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Copy and paste a formatted citation
Spandidos Publications style
Pang X and Hou X: Synergistic protective effect of FTY720 and vitamin E against simulated cerebral ischemia in vitro. Mol Med Rep 16: 396-402, 2017.
APA
Pang, X., & Hou, X. (2017). Synergistic protective effect of FTY720 and vitamin E against simulated cerebral ischemia in vitro. Molecular Medicine Reports, 16, 396-402. https://doi.org/10.3892/mmr.2017.6572
MLA
Pang, X., Hou, X."Synergistic protective effect of FTY720 and vitamin E against simulated cerebral ischemia in vitro". Molecular Medicine Reports 16.1 (2017): 396-402.
Chicago
Pang, X., Hou, X."Synergistic protective effect of FTY720 and vitamin E against simulated cerebral ischemia in vitro". Molecular Medicine Reports 16, no. 1 (2017): 396-402. https://doi.org/10.3892/mmr.2017.6572
Copy and paste a formatted citation
x
Spandidos Publications style
Pang X and Hou X: Synergistic protective effect of FTY720 and vitamin E against simulated cerebral ischemia in vitro. Mol Med Rep 16: 396-402, 2017.
APA
Pang, X., & Hou, X. (2017). Synergistic protective effect of FTY720 and vitamin E against simulated cerebral ischemia in vitro. Molecular Medicine Reports, 16, 396-402. https://doi.org/10.3892/mmr.2017.6572
MLA
Pang, X., Hou, X."Synergistic protective effect of FTY720 and vitamin E against simulated cerebral ischemia in vitro". Molecular Medicine Reports 16.1 (2017): 396-402.
Chicago
Pang, X., Hou, X."Synergistic protective effect of FTY720 and vitamin E against simulated cerebral ischemia in vitro". Molecular Medicine Reports 16, no. 1 (2017): 396-402. https://doi.org/10.3892/mmr.2017.6572
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