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Article

Increased production of human fibroblast growth factor 17 in Escherichia coli and proliferative activity in NIH3T3 cells

  • Authors:
    • Meiyu Wu
    • Na Song
    • Jiliang Cheng
    • Yang Zhao
    • Nazi Chen
    • Jisheng Ma
    • Xiaokun Li
    • Chao Jiang
    • Haijun Wang
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Molecular Biology and Biochemistry, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Biopharmaceuticals, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China
  • Pages: 447-452
    |
    Published online on: May 11, 2017
       https://doi.org/10.3892/mmr.2017.6575
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Abstract

Fibroblast growth factor 17 (FGF17) is a novel member of the FGFs family, which is essential for cell development, tissue repair, tumor growth and invasion. The aim of the current study was to obtain a high expression level of recombinant human FGF17 (rhFGF17), including soluble proteins and inclusion bodies. An optimized rhFGF17 cDNA sequence was cloned into a pET3a vector, then the pET3a‑hFGF17 vector was transformed into BL21(DE3)pLysS Escherichia coli cells. Expression was induced by optimizing the conditions using isopropyl β‑D‑1‑thiogalactopyranoside (IPTG) and it was confirmed that a 24‑h exposure to 0.8 mM IPTG at 16˚C provided the optimal condition for soluble hFGF17. Furthermore, for the inclusion bodies, the optimal condition was a 4‑h exposure to 0.4 mM IPTG at 37˚C. Two forms of rhFGF17 protein were purified by heparin affinity and SP Sepharose Fast Flow chromatography. MTT assays demonstrated that the purified rhFGF17 exerted an important effect on the proliferative activity of NIH3T3 cells, although there was no significant difference when compared with standard rhFGF17. Thus, an optimal and economic expression system was created in the present study for rhFGF17 in E. coli. This expression strategy enables the preparation of sufficient and highly bioactive rhFGF17 for further investigation of underlying mechanisms.
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View References

1 

Ornitz DM and Itoh N: The Fibroblast Growth Factor signaling pathway. Wiley Interdiscip Rev Dev Biol. 4:215–266. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Hoshikawa M, Ohbayashi N, Yonamine A, Konishi M, Ozaki K, Fukui S and Itoh N: Structure and expression of a novel fibroblast growth factor, FGF17, preferentially expressed in the embryonic brain. Biochem Biophys Res Commun. 244:187–191. 1998. View Article : Google Scholar : PubMed/NCBI

3 

Ohbayashi N, Hoshikawa M, Kimura S, Yamasaki M, Fukui S and Itoh N: Structure and expression of the mRNA encoding a novel fibroblast growth factor, FGF-18. J Biol Chem. 273:18161–18164. 1998. View Article : Google Scholar : PubMed/NCBI

4 

Xu J, Lawshe A, MacArthur CA and Ornitz DM: Genomic structure, mapping, activity and expression of fibroblast growth factor 17. Mech Dev. 83:165–178. 1999. View Article : Google Scholar : PubMed/NCBI

5 

O'Leary DD, Chou SJ and Sahara S: Area patterning of the mammalian cortex. Neuron. 56:252–269. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Dong X, Tang B, Li J, Xu Q, Fang S and Hua Z: Expression and purification of intact and functional soybean (Glycine max) seed ferritin complex in Escherichia coli. J Micro Biotech. 18:299–307. 2008.

7 

Jana S and Deb JK: Strategies for efficient production of heterologous proteins in Escherichia coli. Appl Microbiol Biotechnol. 67:289–298. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Derynck R, Roberts AB, Winkler ME, Chen EY and Goeddel DV: Human transforming growth factor-alpha: Precursor structure and expression in E. coli. Cell. 38:287–297. 1984. View Article : Google Scholar : PubMed/NCBI

9 

Verdon J, Girardin N, Marchand A, Héchard Y and Berjeaud JM: Purification and antibacterial activity of recombinant warnericin RK expressed in Escherichia coli. Appl Microbiol Biotechnol. 97:5401–5412. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Ajikumar PK, Xiao WH, Tyo KE, Wang Y, Simeon F, Leonard E, Mucha O, Phon TH and Pfeifer B: Isoprenoid pathway optimization for Taxol precursor overproduction in Escherichia coli. Science. 330:70–74. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Song L, Huang Z, Chen Y, Li H, Jiang C and Li X: High-efficiency production of bioactive recombinant human fibroblast growth factor 18 in Escherichia coli and its effects on hair follicle growth. Appl Microbiol Biotechnol. 98:695–704. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Tabarés-Seisdedos R and Rubenstein JL: Chromosome 8p as a potential hub for developmental neuropsychiatric disorders: Implications for schizophrenia, autism and cancer. Mol Psychiatry. 14:563–589. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Heer R, Douglas D, Mathers ME, Robson CN and Leung HY: Fibroblast growth factor 17 is over-expressed in human prostate cancer. J Pathol. 204:578–586. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Nezu M, Tomonaga T, Sakai C, Ishii A, Itoga S, Nishimura M, Matsuo Y, Tagawa M and Nomura F: Expression of the fetal-oncogenic fibroblast growth factor-8/17/18 subfamily in human hematopoietic tumors. Biochem Biophys Res Commun. 335:843–849. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Hoshikawa M, Ohbayashi N, Yonamine A, Konishi M, Ozaki K, Fukui S and Itoh N: Structure and expression of a novel fibroblast growth factor, FGF-17, preferentially expressed in the embryonic brain. Biochem Biophys Res Commun. 244:187–191. 1998. View Article : Google Scholar : PubMed/NCBI

16 

Sun C, Li Y, Taylor SE, Mao X, Wilkinson MC and Fernig DG: Halo Tag is an effective expression and solubilisation fusion partner for a range of fibroblast growth factors. Peer J. 3:e10602015. View Article : Google Scholar : PubMed/NCBI

17 

de Groot NS and Ventura S: Effect of temperature on protein quality in bacterial inclusion bodies. FEBS Lett. 580:6471–6476. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Berman B, Ostrovsky O, Shlissel M, Lang T, Regan D, Vlodavsky I, Ishai-Michaeli R and Ron D: Similarities and differences between the effects of heparin and glypican-1 on the bioactivity of acidic fibroblast growth factor and the keratinocyte growth factor. J Biol Chem. 274:36132–36138. 1999. View Article : Google Scholar : PubMed/NCBI

19 

Lee YF, Schmidt M, Graalfs H, Hafner M and Frech C: Modeling of dual gradient elution in ion exchange and mixed-mode chromatography. J Chromatogr A. 1417:64–72. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Miraoui H, Dwyer AA, Sykiotis GP, Plummer L, Chung W, Feng B, Beenken A, Clarke J, Pers TH, Dworzynski P, et al: Mutations in FGF17, IL17RD, DUSP6, SPRY4, and FLRT3 are identified in individuals with congenital hypogonadotropic hypogonadism. Am J Hum Genet. 92:725–743. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Meijer D, Sieuwerts AM, Look MP, van Agthoven T, Foekens JA and Dorssers LC: Fibroblast growth factor receptor 4 predicts failure on tamoxifen therapy in patients with recurrent breast cancer. Endocr Relat Cancer. 15:101–111. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wu M, Song N, Cheng J, Zhao Y, Chen N, Ma J, Li X, Jiang C and Wang H: Increased production of human fibroblast growth factor 17 in Escherichia coli and proliferative activity in NIH3T3 cells. Mol Med Rep 16: 447-452, 2017.
APA
Wu, M., Song, N., Cheng, J., Zhao, Y., Chen, N., Ma, J. ... Wang, H. (2017). Increased production of human fibroblast growth factor 17 in Escherichia coli and proliferative activity in NIH3T3 cells. Molecular Medicine Reports, 16, 447-452. https://doi.org/10.3892/mmr.2017.6575
MLA
Wu, M., Song, N., Cheng, J., Zhao, Y., Chen, N., Ma, J., Li, X., Jiang, C., Wang, H."Increased production of human fibroblast growth factor 17 in Escherichia coli and proliferative activity in NIH3T3 cells". Molecular Medicine Reports 16.1 (2017): 447-452.
Chicago
Wu, M., Song, N., Cheng, J., Zhao, Y., Chen, N., Ma, J., Li, X., Jiang, C., Wang, H."Increased production of human fibroblast growth factor 17 in Escherichia coli and proliferative activity in NIH3T3 cells". Molecular Medicine Reports 16, no. 1 (2017): 447-452. https://doi.org/10.3892/mmr.2017.6575
Copy and paste a formatted citation
x
Spandidos Publications style
Wu M, Song N, Cheng J, Zhao Y, Chen N, Ma J, Li X, Jiang C and Wang H: Increased production of human fibroblast growth factor 17 in Escherichia coli and proliferative activity in NIH3T3 cells. Mol Med Rep 16: 447-452, 2017.
APA
Wu, M., Song, N., Cheng, J., Zhao, Y., Chen, N., Ma, J. ... Wang, H. (2017). Increased production of human fibroblast growth factor 17 in Escherichia coli and proliferative activity in NIH3T3 cells. Molecular Medicine Reports, 16, 447-452. https://doi.org/10.3892/mmr.2017.6575
MLA
Wu, M., Song, N., Cheng, J., Zhao, Y., Chen, N., Ma, J., Li, X., Jiang, C., Wang, H."Increased production of human fibroblast growth factor 17 in Escherichia coli and proliferative activity in NIH3T3 cells". Molecular Medicine Reports 16.1 (2017): 447-452.
Chicago
Wu, M., Song, N., Cheng, J., Zhao, Y., Chen, N., Ma, J., Li, X., Jiang, C., Wang, H."Increased production of human fibroblast growth factor 17 in Escherichia coli and proliferative activity in NIH3T3 cells". Molecular Medicine Reports 16, no. 1 (2017): 447-452. https://doi.org/10.3892/mmr.2017.6575
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