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Article Open Access

Differentiation of nestin‑negative human hair follicle outer root sheath cells into neurons in vitro

  • Authors:
    • Wei Wu
    • Xiao‑Li Wu
    • Yu‑Qing Ji
    • Zhen Gao
  • View Affiliations / Copyright

    Affiliations: Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China, Department of Plastic and Reconstructive Surgery, Shanghai General Hospital, Shanghai 200080, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 95-100
    |
    Published online on: May 16, 2017
       https://doi.org/10.3892/mmr.2017.6585
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Abstract

A specialized quiescent population of hair follicle stem cells, residing in the hair follicle outer root sheath cells (ORSCs), has previously demonstrated pluripotency for differentiation into neural stem cells (NSCs). A previous study indicated that nestin‑positive hair follicle ORSCs are able to differentiate into neurons. However, little has been reported on the isolation of nestin‑negative human ORSCs and whether they can successfully differentiate into neurons in vitro. In the present study, nestin‑positive ORSCs were significantly reduced with a prolonged incubation time in vitro. Following 9 days of primary culture, nestin‑expressing ORSCs disappeared entirely, and ORSCs remained nestin‑negative following 5 days of subculture. Notably, nestin was identified in ORSCs following a three‑step process of neuro‑induction. In addition, neruodevelopmental markers were detected in the ORSC‑derived nestin‑positive spherical cell mass, including the induction of the neuronal specific markers growth associated protein‑43, neurotensin receptor‑3 and p75 neurotrophin receptor, and also the gliocyte markers, glial fibrillary acidic protein and S100. These sphere‑forming cells did not express the mature neuron‑associated markers neurofilament medium, neuronal nuclei and neuron‑specific enolase, which suggested that sphere‑forming cells may preferentially differentiate into neural stem cell‑like cells as opposed to mature neurons or neurogliocyte. In conclusion, ORSC‑driven neural differentiation may be a suitable treatment strategy for neurodegenerative diseases and may possess an important value in regenerative medicine.
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1 

Zietlow R, Lane EL, Dunnett SB and Rosser AE: Human stem cells for CNS repair. Cell Tissue Res. 331:301–322. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Ninomiya M, Yamashita T, Araki N, Okano H and Sawamoto K: Enhanced neurogenesis in the ischemic striatum following EGF-induced expansion of transit-amplifying cells in the subventricular zone. Neurosci Lett. 403:63–67. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Batista CE, Mariano ED, Marie SK, Teixeira MJ, Morgalla M, Tatagiba M, Li J and Lepski G: Stem cells in neurology-current perspectives. Arq Neuropsiquiatr. 72:457–465. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Kanno H, Kubo A, Yoshizumi T, Mikami T and Maegawa J: Isolation of multipotent nestin-expressing stem cells derived from the epidermis of elderly humans and TAT-VHL peptide-mediated neuronal differentiation of these cells. Int J Mol Sci. 14:9604–9617. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Savkovic V, Flämig F, Schneider M, Sülflow K, Loth T, Lohrenz A, Hacker MC, Schulz-Siegmund M and Simon JC: Polycaprolactone fiber meshes provide a 3D environment suitable for cultivation and differentiation of melanocytes from the outer root sheath of hair follicle. J Biomed Mater Res A. 104:26–36. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Limat A and Hunziker T: Use of epidermal equivalents generated from follicular outer root sheath cells in vitro and for autologous grafting of chronic wounds. Cells Tissues Organs. 172:79–85. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Liu F, Uchugonova A, Kimura H, Zhang C, Zhao M, Zhang L, Koenig K, Duong J, Aki R, Saito N, et al: The bulge area is the major hair follicle source of nestin-expressing pluripotent stem cells which can repair the spinal cord compared to the dermal papilla. Cell Cycle. 10:830–839. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Amoh Y, Li L, Campillo R, Kawahara K, Katsuoka K, Penman S and Hoffman RM: Implanted hair follicle stem cells form Schwann cells that support repair of severed peripheral nerves. Proc Natl Acad Sci USA. 102:17734–17738. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Amoh Y, Kanoh M, Niiyama S, Hamada Y, Kawahara K, Sato Y, Hoffman RM and Katsuoka K: Human hair follicle pluripotent stem (hfPS) cells promote regeneration of peripheral-nerve injury: An advantageous alternative to ES and iPS cells. J Cell Biochem. 107:1016–1020. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Mazella J: Sortilin/neurotensin receptor-3: A new tool to investigate neurotensin signaling and cellular trafficking? Cell Signal. 13:1–6. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Michalczyk K and Ziman M: Nestin structure and predicted function in cellular cytoskeletal organisation. Histol Histopathol. 20:665–671. 2005.PubMed/NCBI

12 

Yay A, Ozdamar S, Canoz O, Baran M, Tucer B and Sonmez MF: Intermediate filament protein nestin is expressed in developing meninges. Bratisl Lek Listy. 115:718–722. 2014.PubMed/NCBI

13 

Wang Y, Zhang Y, Zeng Y, Zheng Y, Fu G, Cui Z and Yang T: Patterns of nestin expression in human skin. Cell Biol Int. 30:144–148. 2006.PubMed/NCBI

14 

Amoh Y, Li L, Yang M, Moossa AR, Katsuoka K, Penman S and Hoffman RM: Nascent blood vessels in the skin arise from nestin-expressing hair-follicle cells. Proc Natl Acad Sci USA. 101:13291–13295. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Kang H, Tian L, Son YJ, Zuo Y, Procaccino D, Love F, Hayworth C, Trachtenberg J, Mikesh M, Sutton L, et al: Regulation of the intermediate filament protein nestin at rodent neuromuscular junctions by innervation and activity. J Neurosci. 27:5948–5957. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Amoh Y, Li L, Katsuoka K, Penman S and Hoffman RM: Multipotent nestin-positive, keratin-negative hair-follicle bulge stem cells can form neurons. Proc Natl Acad Sci USA. 102:5530–5534. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Yu H, Fang D, Kumar SM, Li L, Nguyen TK, Acs G, Herlyn M and Xu X: Isolation of a novel population of multipotent adult stem cells from human hair follicles. Am J Pathol. 168:1879–1888. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wu W, Wu XL, Ji YQ and Gao Z: Differentiation of nestin‑negative human hair follicle outer root sheath cells into neurons in vitro. Mol Med Rep 16: 95-100, 2017.
APA
Wu, W., Wu, X., Ji, Y., & Gao, Z. (2017). Differentiation of nestin‑negative human hair follicle outer root sheath cells into neurons in vitro. Molecular Medicine Reports, 16, 95-100. https://doi.org/10.3892/mmr.2017.6585
MLA
Wu, W., Wu, X., Ji, Y., Gao, Z."Differentiation of nestin‑negative human hair follicle outer root sheath cells into neurons in vitro". Molecular Medicine Reports 16.1 (2017): 95-100.
Chicago
Wu, W., Wu, X., Ji, Y., Gao, Z."Differentiation of nestin‑negative human hair follicle outer root sheath cells into neurons in vitro". Molecular Medicine Reports 16, no. 1 (2017): 95-100. https://doi.org/10.3892/mmr.2017.6585
Copy and paste a formatted citation
x
Spandidos Publications style
Wu W, Wu XL, Ji YQ and Gao Z: Differentiation of nestin‑negative human hair follicle outer root sheath cells into neurons in vitro. Mol Med Rep 16: 95-100, 2017.
APA
Wu, W., Wu, X., Ji, Y., & Gao, Z. (2017). Differentiation of nestin‑negative human hair follicle outer root sheath cells into neurons in vitro. Molecular Medicine Reports, 16, 95-100. https://doi.org/10.3892/mmr.2017.6585
MLA
Wu, W., Wu, X., Ji, Y., Gao, Z."Differentiation of nestin‑negative human hair follicle outer root sheath cells into neurons in vitro". Molecular Medicine Reports 16.1 (2017): 95-100.
Chicago
Wu, W., Wu, X., Ji, Y., Gao, Z."Differentiation of nestin‑negative human hair follicle outer root sheath cells into neurons in vitro". Molecular Medicine Reports 16, no. 1 (2017): 95-100. https://doi.org/10.3892/mmr.2017.6585
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