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Pre‑treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region

  • Authors:
    • In Hye Kim
    • Tae‑Kyeong Lee
    • Jeong Hwi Cho
    • Jae‑Chul Lee
    • Joon Ha Park
    • Ji Hyeon Ahn
    • Bich‑Na Shin
    • Bai Hui Chen
    • Hyun‑Jin Tae
    • Yang Hee Kim
    • Jong‑Dai Kim
    • Young‑Myeong Kim
    • Moo‑Ho Won
    • Il Jun Kang
  • View Affiliations / Copyright

    Affiliations: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea, Department of Physiology, College of Medicine, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea, Department of Histology and Embryology, Institute of Neuroscience, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China, Bio‑Safety Research Institute, College of Veterinary Medicine, Chonbuk National University, Iksan, Jeollabuk‑do 54596, Republic of Korea, Department of Surgery, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Division of Food Biotechnology, School of Biotechnology, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Food Science and Nutrition, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea
    Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 133-142
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    Published online on: May 17, 2017
       https://doi.org/10.3892/mmr.2017.6591
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Abstract

Chrysanthemum indicum Linné extract (CIL) is used in herbal medicine in East Asia. In the present study, gerbils were orally pre‑treated with CIL, and changes of antioxidant enzymes including superoxide dismutase (SOD) 1 and SOD2, catalase (CAT) and glutathione peroxidase (GPX) in the hippocampal CA1 region following 5 min of transient cerebral ischemia were investigated and the neuroprotective effect of CIL in the ischemic CA1 region was examined. SOD1, SOD2, CAT and GPX immunoreactivities were observed in the pyramidal cells of the CA1 region and their immunoreactivities were gradually decreased following ischemia‑reperfusion and barely detectable at 5 days post‑ischemia. CIL pre‑treatment significantly increased immunoreactivities of SOD1, CAT and GPX, but not SOD2, in the CA1 pyramidal cells of the sham‑operated animals. In addition, SOD1, SOD2, CAT and GPX immunoreactivities in the CA1 pyramidal cells were significantly higher compared with the ischemia‑operated animals. Furthermore, it was identified that pre‑treatment with CIL protected the CA1 pyramidal cells in the CA1 region using neuronal nuclei immunohistochemistry and Fluoro‑Jade B histofluorescence staining; the protected CA1 pyramidal cells were 67.5% compared with the sham‑operated animals. In conclusion, oral CIL pre‑treatment increased endogenous antioxidant enzymes in CA1 pyramidal cells in the gerbil hippocampus and protected the cells from transient cerebral ischemic insult. This finding suggested that CIL is promising for the prevention of ischemia‑induced neuronal damage.
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Copy and paste a formatted citation
Spandidos Publications style
Kim IH, Lee TK, Cho JH, Lee JC, Park JH, Ahn JH, Shin BN, Chen BH, Tae HJ, Kim YH, Kim YH, et al: Pre‑treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region. Mol Med Rep 16: 133-142, 2017.
APA
Kim, I.H., Lee, T., Cho, J.H., Lee, J., Park, J.H., Ahn, J.H. ... Kang, I.J. (2017). Pre‑treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region. Molecular Medicine Reports, 16, 133-142. https://doi.org/10.3892/mmr.2017.6591
MLA
Kim, I. H., Lee, T., Cho, J. H., Lee, J., Park, J. H., Ahn, J. H., Shin, B., Chen, B. H., Tae, H., Kim, Y. H., Kim, J., Kim, Y., Won, M., Kang, I. J."Pre‑treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region". Molecular Medicine Reports 16.1 (2017): 133-142.
Chicago
Kim, I. H., Lee, T., Cho, J. H., Lee, J., Park, J. H., Ahn, J. H., Shin, B., Chen, B. H., Tae, H., Kim, Y. H., Kim, J., Kim, Y., Won, M., Kang, I. J."Pre‑treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region". Molecular Medicine Reports 16, no. 1 (2017): 133-142. https://doi.org/10.3892/mmr.2017.6591
Copy and paste a formatted citation
x
Spandidos Publications style
Kim IH, Lee TK, Cho JH, Lee JC, Park JH, Ahn JH, Shin BN, Chen BH, Tae HJ, Kim YH, Kim YH, et al: Pre‑treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region. Mol Med Rep 16: 133-142, 2017.
APA
Kim, I.H., Lee, T., Cho, J.H., Lee, J., Park, J.H., Ahn, J.H. ... Kang, I.J. (2017). Pre‑treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region. Molecular Medicine Reports, 16, 133-142. https://doi.org/10.3892/mmr.2017.6591
MLA
Kim, I. H., Lee, T., Cho, J. H., Lee, J., Park, J. H., Ahn, J. H., Shin, B., Chen, B. H., Tae, H., Kim, Y. H., Kim, J., Kim, Y., Won, M., Kang, I. J."Pre‑treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region". Molecular Medicine Reports 16.1 (2017): 133-142.
Chicago
Kim, I. H., Lee, T., Cho, J. H., Lee, J., Park, J. H., Ahn, J. H., Shin, B., Chen, B. H., Tae, H., Kim, Y. H., Kim, J., Kim, Y., Won, M., Kang, I. J."Pre‑treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region". Molecular Medicine Reports 16, no. 1 (2017): 133-142. https://doi.org/10.3892/mmr.2017.6591
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