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Article

Increased SLC7A8 expression mediates L-DOPA uptake by renal tubular epithelial cells

  • Authors:
    • Yihao Wu
    • Qiqi Yin
    • Su Lin
    • Xiaoyan Huang
    • Quan Xia
    • Zhe Chen
    • Xingwei Zhang
    • Deye Yang
  • View Affiliations / Copyright

    Affiliations: Division of Cardiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Department of Internal Medicine, The Third People's Hospital at Anji, Huzhou, Zhejiang 313301, P.R. China, Division of Cardiology, The Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang 310015, P.R. China
  • Pages: 887-893
    |
    Published online on: May 25, 2017
       https://doi.org/10.3892/mmr.2017.6620
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Abstract

The kidney serves a central role in the control of blood pressure through the release of vasoactive substances and the urinary excretion of Na+. Patients with essential hypertension usually exhibit persistent high blood pressure accompanied by Na+ retention. L-dihydroxyphenylalanine (L‑DOPA) is an amino acid, converted by the enzyme aromatic L‑amino acid decarboxylase to dopamine. The uptake of L‑DOPA by cells of the proximal tubular epithelium of the kidney is controlled by the L‑type amino acid transporter 2 (LAT2). LAT2 belongs to the solute carrier family 7 (SLC7) of amino acid transporters and is coded by the SLC7A8 gene. SLC7A8 expression is increased in the second‑order mesenteric arteries and kidneys of spontaneously hypertensive rats. The present study aimed to investigate the physiological role of the SLC7A8 gene in L‑DOPA handling by kidney cells. Selective upregulation of SLC7A8 mRNA and protein levels was achieved by adenoviral transduction of NRK‑52E cells, which retain several properties of proximal tubular epithelial cells. In addition, L‑DOPA uptake was determined using high performance liquid chromatography; NRK‑52E cells expressing SLC7A8 exhibited increased uptake of L‑DOPA. The results of the present study suggested that SLC7A8 may serve a critical role in blood pressure control through regulating L‑DOPA uptake in renal epithelial cells of the proximal tubule.
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Copy and paste a formatted citation
Spandidos Publications style
Wu Y, Yin Q, Lin S, Huang X, Xia Q, Chen Z, Zhang X and Yang D: Increased SLC7A8 expression mediates L-DOPA uptake by renal tubular epithelial cells. Mol Med Rep 16: 887-893, 2017.
APA
Wu, Y., Yin, Q., Lin, S., Huang, X., Xia, Q., Chen, Z. ... Yang, D. (2017). Increased SLC7A8 expression mediates L-DOPA uptake by renal tubular epithelial cells. Molecular Medicine Reports, 16, 887-893. https://doi.org/10.3892/mmr.2017.6620
MLA
Wu, Y., Yin, Q., Lin, S., Huang, X., Xia, Q., Chen, Z., Zhang, X., Yang, D."Increased SLC7A8 expression mediates L-DOPA uptake by renal tubular epithelial cells". Molecular Medicine Reports 16.1 (2017): 887-893.
Chicago
Wu, Y., Yin, Q., Lin, S., Huang, X., Xia, Q., Chen, Z., Zhang, X., Yang, D."Increased SLC7A8 expression mediates L-DOPA uptake by renal tubular epithelial cells". Molecular Medicine Reports 16, no. 1 (2017): 887-893. https://doi.org/10.3892/mmr.2017.6620
Copy and paste a formatted citation
x
Spandidos Publications style
Wu Y, Yin Q, Lin S, Huang X, Xia Q, Chen Z, Zhang X and Yang D: Increased SLC7A8 expression mediates L-DOPA uptake by renal tubular epithelial cells. Mol Med Rep 16: 887-893, 2017.
APA
Wu, Y., Yin, Q., Lin, S., Huang, X., Xia, Q., Chen, Z. ... Yang, D. (2017). Increased SLC7A8 expression mediates L-DOPA uptake by renal tubular epithelial cells. Molecular Medicine Reports, 16, 887-893. https://doi.org/10.3892/mmr.2017.6620
MLA
Wu, Y., Yin, Q., Lin, S., Huang, X., Xia, Q., Chen, Z., Zhang, X., Yang, D."Increased SLC7A8 expression mediates L-DOPA uptake by renal tubular epithelial cells". Molecular Medicine Reports 16.1 (2017): 887-893.
Chicago
Wu, Y., Yin, Q., Lin, S., Huang, X., Xia, Q., Chen, Z., Zhang, X., Yang, D."Increased SLC7A8 expression mediates L-DOPA uptake by renal tubular epithelial cells". Molecular Medicine Reports 16, no. 1 (2017): 887-893. https://doi.org/10.3892/mmr.2017.6620
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