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Article Open Access

Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments

  • Authors:
    • Chaofan Sun
    • Fen Liu
    • Shendan Cen
    • Lijiao Chen
    • Yi Wang
    • Hao Sun
    • Hui Deng
    • Rongdang Hu
  • View Affiliations / Copyright

    Affiliations: Department of Orthodontics, School of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China, Department of Histology and Embryology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China, Department of Periodontics, School of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China, Faculty of Dentistry, University of Hong Kong, Hong Kong 999077, SAR, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 666-672
    |
    Published online on: May 29, 2017
       https://doi.org/10.3892/mmr.2017.6644
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Abstract

The present study aimed to investigate the role of orthodontic force in osteogenesis differentiation, matrix deposition and mineralization in periodontal ligament cells (PDLCs) cells in inflammatory microenvironments. The mesenchymal origin of PDLCs was confirmed by vimentin and cytokeratin staining. PDLCs were exposed to inflammatory cytokines (5 ng/ml IL‑1β and 10 ng/ml TNF‑α) and/or tensile strength (0.5 Hz, 12% elongation) for 12, 24 or 48 h. Cell proliferation and tensile strength‑induced cytokine expression were assessed by MTT assay and ELISA, respectively. Runt‑related transcription factor 2 (RUNX2) and type I collagen (COL‑I) expression were analysed by reverse transcription‑quantitative polymerase chain reaction and western blot analysis. Additionally, alkaline phosphatase activity was measured, and the mineralization profile was evaluated by alizarin red S staining. PDLCs exposed to tensile strength in inflammatory microenvironments exhibited reduced proliferation and mineralization potential. Treatment with the inflammatory cytokines IL‑1β and TNF‑α increased RUNX2 expression levels; however, decreased COL‑I expression levels, indicating that bone formation and matrix deposition involve different mechanisms in PDL tissues. Notably, RUNX2 and COL‑I expression levels were decreased in PDLCs exposed to a combination of an inflammatory environment and loading strength. The decreased osteogenic potential in an inflammatory microenvironment under tensile strength suggests that orthodontic force may amplify periodontal destruction in orthodontic patients with periodontitis.
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View References

1 

Ivanovski S, Gronthos S, Shi S and Bartold PM: Stem cells in the periodontal ligament. Oral Dis. 12:358–363. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Agarwal S, Long P, Seyedain A, Piesco N, Shree A and Gassner R: A central role for the nuclear factor-kappaB pathway in anti-inflammatory and proinflammatory actions of mechanical strain. FASEB J. 17:899–901. 2003.PubMed/NCBI

3 

Liu M, Dai J, Lin Y, Yang L, Dong H, Li Y, Ding Y and Duan Y: Effect of the cyclic stretch on the expression of osteogenesis genes in human periodontal ligament cells. Gene. 491:187–193. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Lee YH, Nahm DS, Jung YK, Choi JY and Kim SG, Cho M, Kim MH, Chae CH and Kim SG: Differential gene expression of periodontal ligament cells after loading of static compressive force. J Periodontol. 78:446–452. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Nakao K, Goto T, Gunjigake KK, Konoo T, Kobayashi S and Yamaguchi K: Intermittent force induces high RANKL expression in human periodontal ligament cells. J Dent Res. 86:623–628. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Li J, Jiang L, Liao G, Chen G, Liu Y, Wang J, Zheng Y, Luo S and Zhao Z: Centrifugal forces within usually-used magnitude elicited a transitory and reversible change in proliferation and gene expression of osteoblastic cells UMR-106. Mol Biol Rep. 36:299–305. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Yang YQ, Li XT, Rabie AB, Fu MK and Zhang D: Human periodontal ligament cells express osteoblastic phenotypes under intermittent force loading in vitro. Front Biosci. 11:776–781. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Okada H and Murakami S: Cytokine expression in periodontal health and disease. Crit Rev Oral Biol Med. 9:248–266. 1998. View Article : Google Scholar : PubMed/NCBI

9 

Gamonal J, Acevedo A, Bascones A, Jorge O and Silva A: Levels of interleukin-1 beta, -8 and -10 and RANTES in gingival crevicular fluid and cell populations in adult periodontitis patients and the effect of periodontal treatment. J Periodontol. 71:1535–1545. 2000. View Article : Google Scholar : PubMed/NCBI

10 

Delima AJ, Oates T, Assuma R, Schwartz Z, Cochran D, Amar S and Graves DT: Soluble antagonists to interleukin-1 (IL-1) and tumor necrosis factor (TNF) inhibits loss of tissue attachment in experimental periodontitis. J Clin Periodontol. 28:233–240. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Graves DT and Cochran D: The contribution of interleukin-1 and tumor necrosis factor to periodontal tissue destruction. J Periodontol. 74:391–401. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Górska R, Gregorek H, Kowalski J, Laskus-Perendyk A, Syczewska M and Madalinski K: Relationship between clinical parameters and cytokine profiles in inflamed gingival tissue and serum samples from patients with chronic periodontitis. J Clin Periodontol. 30:1046–1052. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Liu N, Shi S, Deng M, Tang L, Zhang G, Liu N, Ding B, Liu W, Liu Y, Shi H, et al: High levels of β-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway. J Bone Miner Res. 26:2082–2095. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Kim YS, Pi SH, Lee YM, Lee SI and Kim EC: The anti-inflammatory role of heme oxygenase-1 in lipopolysaccharide and cytokine-stimulated inducible nitric oxide synthase and nitric oxide production in human periodontal ligament cells. J Periodontol. 80:2045–2055. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Yang H, Gao LN, An Y, Hu CH, Jin F, Zhou J, Jin Y and Chen FM: Comparison of mesenchymal stem cells derived from gingival tissue and periodontal ligament in different incubation conditions. Biomaterials. 34:7033–7047. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Omar OM, Granéli C, Ekström K, Karlsson C, Johansson A, Lausmaa J, Wexell CL and Thomsen P: The stimulation of an osteogenic response by classical monocyte activation. Biomaterials. 32:8190–8204. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Ekström K, Omar O, Granéli C, Wang X, Vazirisani F and Thomsen P: Monocyte exosomes stimulate the osteogenic gene expression of mesenchymal stem cells. PLoS One. 8:e752272013. View Article : Google Scholar : PubMed/NCBI

18 

Rossa C Jr, Liu M, Patil C and Kirkwood KL: MKK3/6-p38 MAPK negatively regulates murine MMP-13 gene expression induced by IL-1beta and TNF-alpha in immortalized periodontal ligament fibroblasts. Matrix Biol. 24:478–488. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Wang Y, Tang Z, Xue R, Singh GK, Shi K, Lv Y and Yang L: Combined effects of TNF-α, IL-1β and HIF-1α on MMP-2 production in ACL fibroblasts under mechanical stretch: An in vitro study. J Orthop Res. 29:1008–1014. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Tsuji K, Uno K, Zhang GX and Tamura M: Periodontal ligament cells under intermittent tensile stress regulate mRNA expression of osteoprotegerin and tissue inhibitor of matrix metalloprotease-1 and -2. J Bone Miner Metab. 22:94–103. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Kusano K, Miyaura C, Inada M, Tamura T, Ito A, Nagase H, Kamoi K and Suda T: Regulation of matrix metalloproteinases (MMP-2, -3, -9 and -13) by interleukin-1 and interleukin-6 in mouse calvaria: Association of MMP induction with bone resorption. Endocrinology. 139:1338–1345. 1998. View Article : Google Scholar : PubMed/NCBI

22 

Ma J, Kitti U, Teronen O, Sorsa T, Husa V, Laine P, Rönkä H, Salo T, Lindqvist C and Konttinen YT: Collagenases in different categories of peri-implant vertical bone loss. J Dent Res. 79:1870–1873. 2000. View Article : Google Scholar : PubMed/NCBI

23 

Lian JB and Stein GS: Runx2/Cbfa1: A multifunctional regulator of bone formation. Curr Pharm Des. 9:2677–2685. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Matsuda N, Yokoyama K, Takeshita S and Watanabe M: Role of epidermal growth factor and its receptor in mechanical stress-induced differentiation of human periodontal ligament cells in vitro. Arch Oral Biol. 43:987–997. 1998. View Article : Google Scholar : PubMed/NCBI

25 

Kawarizadeh A, Bourauel C, Götz W and Jäger A: Early responses of periodontal ligament cells to mechanical stimulus in vivo. J Dent Res. 84:902–906. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Chen B, Sun HH, Wang HG, Kong H, Chen FM and Yu Q: The effects of human platelet lysate on dental pulp stem cells derived from impacted human third molars. Biomaterials. 33:5023–5035. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Wescott DC, Pinkerton MN, Gaffey BJ, Beggs KT, Milne TJ and Meikle MC: Osteogenic gene expression by human periodontal ligament cells under cyclic tension. J Dent Res. 86:1212–1216. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Han LM, Li S, Wang L and Xu Y: Cyclic tensile stress during physiological occlusal force enhances osteogenic differentiation of human periodontal ligament cells via ERK1/2-Elk1 MAPK pathway. DNA Cell Biol. 32:488–497. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Natali AN, Pavan PG and Scarpa C: Numerical analysis of tooth mobility: Formulation of a non-linear constitutive law for the periodontal ligament. Dent Mater. 20:623–629. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Yeh Y, Yang Y and Yuan K: Importance of CD44 in the proliferation and mineralization of periodontal ligament cells. J Periodontal Res. 49:827–835. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Pabari S, Moles DR and Cunningham SJ: Assessment of motivation and psychological characteristics of adult orthodontic patients. Am J Orthod Dentofacial Orthop. 140:e263–e272. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Hajishengallis G: Aging and its impact on innate immunity and inflammation: Implications for periodontitis. J Oral Biosci. 56:30–37. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Eke PI, Dye BA, Wei L, Thornton-Evans GO and Genco RJ: CDC Periodontal Disease Surveillance workgroup: James Beck (University of North Carolina, Chapel Hill, USA), Gordon Douglass (Past President, American Academy of Periodontology), Roy Page (University of Washin): Prevalence of periodontitis in adults in the United States: 2009 and 2010. J Dent Res. 91:914–920. 2012. View Article : Google Scholar : PubMed/NCBI

34 

Wang Y, Wang H, Ye Q, Ye J, Xu C, Lin L, Deng H and Hu R: Co-regulation of LPS and tensile strain downregulating osteogenicity via c-fos expression. Life Sci. 93:38–43. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Yamaguchi M, Shimizu N, Shibata Y and Abiko Y: Effects of different magnitudes of tension-force on alkaline phosphatase activity in periodontal ligament cells. J Dent Res. 75:889–894. 1996. View Article : Google Scholar : PubMed/NCBI

36 

Long P, Hu J, Piesco N, Buckley M and Agarwal S: Low magnitude of tensile strain inhibits IL-1beta-dependent induction of pro-inflammatory cytokines and induces synthesis of IL-10 in human periodontal ligament cells in vitro. J Dent Res. 80:1416–1420. 2001. View Article : Google Scholar : PubMed/NCBI

37 

Baba S, Kuroda N, Arai C, Nakamura Y and Sato T: Immunocompetent cells and cytokine expression in the rat periodontal ligament at the initial stage of orthodontic tooth movement. Arch Oral Biol. 56:466–473. 2011. View Article : Google Scholar : PubMed/NCBI

38 

Bletsa A, Berggreen E and Brudvik P: Interleukin-1alpha and tumor necrosis factor-alpha expression during the early phases of orthodontic tooth movement in rats. Eur J Oral Sci. 114:423–429. 2006. View Article : Google Scholar : PubMed/NCBI

39 

Nokhbehsaim M, Deschner B, Winter J, Reimann S, Bourauel C, Jepsen S, Jäger A and Deschner J: Contribution of orthodontic load to inflammation-mediated periodontal destruction. J Orofac Orthop. 71:390–402. 2010. View Article : Google Scholar : PubMed/NCBI

40 

Rifas L: T-cell cytokine induction of BMP-2 regulates human mesenchymal stromal cell differentiation and mineralization. J Cell Biochem. 98:706–714. 2006. View Article : Google Scholar : PubMed/NCBI

41 

Rifas L, Arackal S and Weitzmann MN: Inflammatory T cells rapidly induce differentiation of human bone marrow stromal cells into mature osteoblasts. J Cell Biochem. 88:650–659. 2003. View Article : Google Scholar : PubMed/NCBI

42 

Nemoto T, Kajiya H, Tsuzuki T, Takahashi Y and Okabe K: Differential induction of collagens by mechanical stress in human periodontal ligament cells. Arch Oral Biol. 55:981–987. 2010. View Article : Google Scholar : PubMed/NCBI

43 

Ding J, Ghali O, Lencel P, Broux O, Chauveau C, Devedjian JC, Hardouin P and Magne D: TNF-alpha and IL-1beta inhibit RUNX2 and collagen expression but increase alkaline phosphatase activity and mineralization in human mesenchymal stem cells. Life Sci. 84:499–504. 2009. View Article : Google Scholar : PubMed/NCBI

44 

Zhang P, Wu Y, Jiang Z, Jiang L and Fang B: Osteogenic response of mesenchymal stem cells to continuous mechanical strain is dependent on ERK1/2-Runx2 signaling. Int J Mol Med. 29:1083–1089. 2012.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Sun C, Liu F, Cen S, Chen L, Wang Y, Sun H, Deng H and Hu R: Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments. Mol Med Rep 16: 666-672, 2017.
APA
Sun, C., Liu, F., Cen, S., Chen, L., Wang, Y., Sun, H. ... Hu, R. (2017). Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments. Molecular Medicine Reports, 16, 666-672. https://doi.org/10.3892/mmr.2017.6644
MLA
Sun, C., Liu, F., Cen, S., Chen, L., Wang, Y., Sun, H., Deng, H., Hu, R."Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments". Molecular Medicine Reports 16.1 (2017): 666-672.
Chicago
Sun, C., Liu, F., Cen, S., Chen, L., Wang, Y., Sun, H., Deng, H., Hu, R."Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments". Molecular Medicine Reports 16, no. 1 (2017): 666-672. https://doi.org/10.3892/mmr.2017.6644
Copy and paste a formatted citation
x
Spandidos Publications style
Sun C, Liu F, Cen S, Chen L, Wang Y, Sun H, Deng H and Hu R: Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments. Mol Med Rep 16: 666-672, 2017.
APA
Sun, C., Liu, F., Cen, S., Chen, L., Wang, Y., Sun, H. ... Hu, R. (2017). Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments. Molecular Medicine Reports, 16, 666-672. https://doi.org/10.3892/mmr.2017.6644
MLA
Sun, C., Liu, F., Cen, S., Chen, L., Wang, Y., Sun, H., Deng, H., Hu, R."Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments". Molecular Medicine Reports 16.1 (2017): 666-672.
Chicago
Sun, C., Liu, F., Cen, S., Chen, L., Wang, Y., Sun, H., Deng, H., Hu, R."Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments". Molecular Medicine Reports 16, no. 1 (2017): 666-672. https://doi.org/10.3892/mmr.2017.6644
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