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The expression of DNA methyltransferases3A is specifically downregulated in chorionic villi of early embryo growth arrest cases

  • Authors:
    • Huating Gu
    • Jing Gao
    • Weiwei Guo
    • Yu Zhou
    • Qingnuan Kong
  • View Affiliations / Copyright

    Affiliations: Department of Physiology and Pathophysiology, Medical College of Qingdao University, Qingdao, Shandong 266071, P.R. China, Department of Reproductive Medicine Center, Qingdao Municipal Hospital, Affiliated to Medical College of Qingdao University, Qingdao, Shandong 266011, P.R. China, Department of Pathology, Yantaishan Hospital, Affiliated to Medical College of Qingdao University, Yantai, Shandong 264001, P.R. China, Department of Pathology, Qingdao Municipal Hospital, Affiliated to Medical College of Qingdao University, Qingdao, Shandong 266011, P.R. China
    Copyright: © Gu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 591-596
    |
    Published online on: May 30, 2017
       https://doi.org/10.3892/mmr.2017.6650
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Abstract

The aim of the present study was to investigate the expression pattern of four DNA methyltransferases (DNMT1, DNMT3A, DNMT3B and DNMT3L) in placenta chorionic villi of early embryo growth arrest patients. Chorionic villous specimens were obtained from 40 pregnant patients diagnosed with early embryo growth arrest and 40 healthy women who underwent selective pregnancy termination. Reverse transcription‑quantitative polymerase chain reaction, immunohistochemistry and western blot analysis were performed to characterize the mRNA and protein expression of DNMTs in chorionic villous cells. It was identified, among the four DNMTs, DNMT3B presented the highest level of protein expression in both patient groups. Although the mRNA expressions of the four DNMTs were comparable, the DNMT3A protein was specifically downregulated in patients with early embryo growth arrest. Therefore, the current study suggests that an abnormal decrease in DNMT3A protein levels may be involved in the pathogenesis of early embryo growth arrest.
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Copy and paste a formatted citation
Spandidos Publications style
Gu H, Gao J, Guo W, Zhou Y and Kong Q: The expression of DNA methyltransferases3A is specifically downregulated in chorionic villi of early embryo growth arrest cases. Mol Med Rep 16: 591-596, 2017.
APA
Gu, H., Gao, J., Guo, W., Zhou, Y., & Kong, Q. (2017). The expression of DNA methyltransferases3A is specifically downregulated in chorionic villi of early embryo growth arrest cases. Molecular Medicine Reports, 16, 591-596. https://doi.org/10.3892/mmr.2017.6650
MLA
Gu, H., Gao, J., Guo, W., Zhou, Y., Kong, Q."The expression of DNA methyltransferases3A is specifically downregulated in chorionic villi of early embryo growth arrest cases". Molecular Medicine Reports 16.1 (2017): 591-596.
Chicago
Gu, H., Gao, J., Guo, W., Zhou, Y., Kong, Q."The expression of DNA methyltransferases3A is specifically downregulated in chorionic villi of early embryo growth arrest cases". Molecular Medicine Reports 16, no. 1 (2017): 591-596. https://doi.org/10.3892/mmr.2017.6650
Copy and paste a formatted citation
x
Spandidos Publications style
Gu H, Gao J, Guo W, Zhou Y and Kong Q: The expression of DNA methyltransferases3A is specifically downregulated in chorionic villi of early embryo growth arrest cases. Mol Med Rep 16: 591-596, 2017.
APA
Gu, H., Gao, J., Guo, W., Zhou, Y., & Kong, Q. (2017). The expression of DNA methyltransferases3A is specifically downregulated in chorionic villi of early embryo growth arrest cases. Molecular Medicine Reports, 16, 591-596. https://doi.org/10.3892/mmr.2017.6650
MLA
Gu, H., Gao, J., Guo, W., Zhou, Y., Kong, Q."The expression of DNA methyltransferases3A is specifically downregulated in chorionic villi of early embryo growth arrest cases". Molecular Medicine Reports 16.1 (2017): 591-596.
Chicago
Gu, H., Gao, J., Guo, W., Zhou, Y., Kong, Q."The expression of DNA methyltransferases3A is specifically downregulated in chorionic villi of early embryo growth arrest cases". Molecular Medicine Reports 16, no. 1 (2017): 591-596. https://doi.org/10.3892/mmr.2017.6650
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