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Article

An FGF8b-mimicking peptide with potent antiangiogenic activity

  • Authors:
    • Xiaomian Lin
    • Li Song
    • Dan He
    • Xiangfeng Zeng
    • Jianzhang Wu
    • Wu Luo
    • Qi Yang
    • Jizhong Wang
    • Tianxiang Wang
    • Jialong Cai
    • Yanling Lin
    • Fubin Lai
    • Wentao Peng
    • Xiaoping Wu
  • View Affiliations / Copyright

    Affiliations: Institute of Tissue Transplantation and Immunology, Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes and Key Laboratory of Molecule Immunology and Antibody Engineering of Guangdong Province, Jinan University, Guangzhou, Guangdong 510632, P.R. China, Department of Pharmacy, School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China
  • Pages: 894-900
    |
    Published online on: May 30, 2017
       https://doi.org/10.3892/mmr.2017.6651
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Abstract

Fibroblast growth factor (FGF) 8b interacts with its receptors and promotes angiogenesis in hormone‑dependent tumors. In the present study, we demonstrated that a short peptide, termed 8b‑13, which mimics part of the FGF8b structure, significantly inhibited the proliferation and migration of human umbilical vein endothelial cells (HUVECs) triggered by FGF8b using 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT), flow cytometry and an in vitro scratch assay. In addition, the findings from western blotting and reverse transcription‑quantitative polymerase chain reaction revealed that 8b‑13 appeared to counteract the effects of FGF8b on the expression of cyclin D1, the activation of signaling cascades, and the expression of proangiogenic factors; these actions may be involved in the mechanism underlying the inhibitory effects of 8b‑13 on FGF8b‑induced HUVEC proliferation and migration. The present results suggested that 8b‑13 may be considered a potent FGF8b antagonist with antiangiogenic activity, and may have potential as a novel therapeutic agent for the treatment of cancer characterized by abnormal FGF8b upregulation.
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1 

Mattila MM, Ruohola JK, Valve EM, Tasanen MJ, Seppänen JA and Härkönen PL: FGF-8b increases angiogenic capacity and tumor growth of androgen-regulated S115 breast cancer cells. Oncogene. 20:2791–2804. 2001. View Article : Google Scholar : PubMed/NCBI

2 

Valta MP, Tuomela J, Vuorikoski H, Loponen N, Väänänen RM, Pettersson K, Väänänen HK and Härkönen PL: FGF-8b induces growth and rich vascularization in an orthotopic PC-3 model of prostate cancer. J Cell Biochem. 107:769–784. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Ruohola JK, Viitanen TP, Valve EM, Seppänen JA, Loponen NT, Keskitalo JJ, Lakkakorpi PT and Härkönen PL: Enhanced invasion and tumor growth of fibroblast growth factor 8b-overexpressing MCF-7 human breast cancer cells. Cancer Res. 61:4229–4237. 2001.PubMed/NCBI

4 

Tuomela J, Grönroos TJ, Valta MP, Sandholm J, Schrey A, Seppänen J, Marjamäki P, Forsback S, Kinnunen I, Solin O, et al: Fast growth associated with aberrant vasculature and hypoxia in fibroblast growth factor 8b (FGF8b) over-expressing PC-3 prostate tumour xenografts. BMC Cancer. 10:5962010. View Article : Google Scholar : PubMed/NCBI

5 

Valve E, Martikainen P, Seppänen J, Oksjoki S, Hinkka S, Anttila L, Grenman S, Klemi P and Härkönen P: Expression of fibroblast growth factor (FGF)-8 isoforms and FGF receptors in human ovarian tumors. Int J Cancer. 88:718–725. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Maruyama-Takahashi K, Shimada N, Imada T, Maekawa-Tokuda Y, Ishii T, Ouchi J, Kusaka H, Miyaji H, Akinaga S, Tanaka A and Shitara K: A neutralizing anti-fibroblast growth factor (FGF) 8 monoclonal antibody shows anti-tumor activity against FGF8b-expressing LNCaP xenografts in androgen-dependent and -independent conditions. Prostate. 68:640–650. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Mattila MM and Härkönen PL: Role of fibroblast growth factor 8 in growth and progression of hormonal cancer. Cytokine Growth Factor Rev. 18:257–266. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Valta MP, Tuomela J, Bjartell A, Valve E, Väänänen HK and Härkönen P: FGF-8 is involved in bone metastasis of prostate cancer. Int J Cancer. 123:22–31. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Li T, Luo W, He D, Wang R, Huang Y, Zeng X, Wang W, Chen X, Gao S, Yu Y, et al: A short peptide derived from the gN helix domain of FGF8b suppresses the growth of human prostate cancer cells. Cancer Lett. 339:226–236. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Baldin V, Lukas J, Marcote MJ, Pagano M and Draetta G: Cyclin D1 is a nuclear protein required for cell cycle progression in G1. Genes Dev. 7:812–821. 1993. View Article : Google Scholar : PubMed/NCBI

12 

Hadari Y, Gotoh N, Kouhara H, Lax I and Schlessinger J: Critical role for the docking-protein FRS2α in FGF receptor-mediated signal transduction pathways. Proc Natl Acad Sci USA. 98:8578–8583. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Ong SH, Guy GR, Hadari YR, Laks S, Gotoh N, Schlessinger J and Lax I: FRS2 proteins recruit intracellular signaling pathways by binding to diverse targets on fibroblast growth factor and nerve growth factor receptors. Mol Cell Biol. 20:979–989. 2000. View Article : Google Scholar : PubMed/NCBI

14 

Yang X, Qiao D, Meyer K and Friedl A: Signal transducers and activators of transcription mediate fibroblast growth factor-induced vascular endothelial morphogenesis. Cancer Res. 69:1668–1677. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Yang X, Qiao D, Meyer K, Pier T, Keles S and Friedl A: Angiogenesis induced by signal transducer and activator of transcription 5A (STAT5A) is dependent on autocrine activity of proliferin. J Biol Chem. 287:6490–6502. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Folkman J: Role of angiogenesis in tumor growth and metastasis. Semin Oncol. 29:(6 Suppl 16). S15–S18. 2002. View Article : Google Scholar

17 

Folkman J: Angiogenesis. Annu Rev Med. 57:1–18. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Ucuzian AA and Greisler HP: In vitro models of angiogenesis. World J Surg. 31:654–663. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Antoine M, Wirz W, Tag CG, Mavituna M, Emans N, Korff T, Stoldt V, Gressner AM and Kiefer P: Expression pattern of fibroblast growth factors (FGFs), their receptors and antagonists in primary endothelial cells and vascular smooth muscle cells. Growth Factors. 23:87–95. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Nilsson EM, Brokken LJ and Härkönen PL: Fibroblast growth factor 8 increases breast cancer cell growth by promoting cell cycle progression and by protecting against cell death. Exp Cell Res. 316:800–812. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Matsumura I, Kitamura T, Wakao H, Tanaka H, Hashimoto K, Albanese C, Downward J, Pestell RG and Kanakura Y: Transcriptional regulation of the cyclin D1 promoter by STAT5: Its involvement in cytokine-dependent growth of hematopoietic cells. EMBO J. 18:1367–1377. 1999. View Article : Google Scholar : PubMed/NCBI

22 

Dudley AC, Thomas D, Best J and Jenkins A: A VEGF/JAK2/STAT5 axis may partially mediate endothelial cell tolerance to hypoxia. Biochem J. 390:427–436. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Klemke RL, Cai S, Giannini AL, Gallagher PJ, de Lanerolle P and Cheresh DA: Regulation of cell motility by mitogen-activated protein kinase. J Cell Biol. 137:481–492. 1997. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lin X, Song L, He D, Zeng X, Wu J, Luo W, Yang Q, Wang J, Wang T, Cai J, Cai J, et al: An FGF8b-mimicking peptide with potent antiangiogenic activity. Mol Med Rep 16: 894-900, 2017.
APA
Lin, X., Song, L., He, D., Zeng, X., Wu, J., Luo, W. ... Wu, X. (2017). An FGF8b-mimicking peptide with potent antiangiogenic activity. Molecular Medicine Reports, 16, 894-900. https://doi.org/10.3892/mmr.2017.6651
MLA
Lin, X., Song, L., He, D., Zeng, X., Wu, J., Luo, W., Yang, Q., Wang, J., Wang, T., Cai, J., Lin, Y., Lai, F., Peng, W., Wu, X."An FGF8b-mimicking peptide with potent antiangiogenic activity". Molecular Medicine Reports 16.1 (2017): 894-900.
Chicago
Lin, X., Song, L., He, D., Zeng, X., Wu, J., Luo, W., Yang, Q., Wang, J., Wang, T., Cai, J., Lin, Y., Lai, F., Peng, W., Wu, X."An FGF8b-mimicking peptide with potent antiangiogenic activity". Molecular Medicine Reports 16, no. 1 (2017): 894-900. https://doi.org/10.3892/mmr.2017.6651
Copy and paste a formatted citation
x
Spandidos Publications style
Lin X, Song L, He D, Zeng X, Wu J, Luo W, Yang Q, Wang J, Wang T, Cai J, Cai J, et al: An FGF8b-mimicking peptide with potent antiangiogenic activity. Mol Med Rep 16: 894-900, 2017.
APA
Lin, X., Song, L., He, D., Zeng, X., Wu, J., Luo, W. ... Wu, X. (2017). An FGF8b-mimicking peptide with potent antiangiogenic activity. Molecular Medicine Reports, 16, 894-900. https://doi.org/10.3892/mmr.2017.6651
MLA
Lin, X., Song, L., He, D., Zeng, X., Wu, J., Luo, W., Yang, Q., Wang, J., Wang, T., Cai, J., Lin, Y., Lai, F., Peng, W., Wu, X."An FGF8b-mimicking peptide with potent antiangiogenic activity". Molecular Medicine Reports 16.1 (2017): 894-900.
Chicago
Lin, X., Song, L., He, D., Zeng, X., Wu, J., Luo, W., Yang, Q., Wang, J., Wang, T., Cai, J., Lin, Y., Lai, F., Peng, W., Wu, X."An FGF8b-mimicking peptide with potent antiangiogenic activity". Molecular Medicine Reports 16, no. 1 (2017): 894-900. https://doi.org/10.3892/mmr.2017.6651
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