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Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway

  • Authors:
    • Xiaoying Gao
    • Yun Liu
    • Yuying Xie
    • Ying Wang
    • Sihua Qi
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 515-522
    |
    Published online on: May 30, 2017
       https://doi.org/10.3892/mmr.2017.6652
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Abstract

Ischemic postconditioning has been demonstrated to alleviate brain ischemia/reperfusion-induced neuronal apoptosis; however, the protective mechanisms underlying the improved and more convenient method of remote ischemic postconditioning (RIPostC) are only recently beginning to be elucidated. Mitochondria are important in the regulation of cell apoptosis, and the B‑cell lymphoma 2 (Bcl‑2) homology 3 interacting‑domain death agonist (BID) promotes the insertion/oligomerization of Bcl‑2‑associated X protein into the mitochondrial outer membrane, leading to the release of proapoptotic proteins from the mitochondria. The present study hypothesized that RIPostC targets the BID‑mediated mitochondrial apoptotic pathway to exert neuroprotective effects, and the optimal time window for RIPostC application was investigated. RIPostC was conducted as follows: Three 10‑min cycles of bilateral femoral artery occlusion with intervals of 10 min reperfusion after 0, 10 or 30 min of brain reperfusion. The results revealed that reperfusion induced significant activation of BID, via proteolytic cleavage and translocation to the mitochondria, as determined using western blot analysis and immunofluorescence staining. Mitochondrial release of cytochrome c was additionally detected during BID activation, all of which were inhibited by the application of RIPostC. When RIPostC was applied during reperfusion, it demonstrated a significant protective effect. Furthermore, the infarct volume, neurological function and the degree of neuronal apoptosis were improved with application of RIPostC. These results suggested that the protective mechanisms of RIPostC may be associated with inhibition of the BID‑mediated mitochondrial apoptotic pathway, which may act as a potential molecular target for therapeutic intervention in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Gao X, Liu Y, Xie Y, Wang Y and Qi S: Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway. Mol Med Rep 16: 515-522, 2017.
APA
Gao, X., Liu, Y., Xie, Y., Wang, Y., & Qi, S. (2017). Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway. Molecular Medicine Reports, 16, 515-522. https://doi.org/10.3892/mmr.2017.6652
MLA
Gao, X., Liu, Y., Xie, Y., Wang, Y., Qi, S."Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway". Molecular Medicine Reports 16.1 (2017): 515-522.
Chicago
Gao, X., Liu, Y., Xie, Y., Wang, Y., Qi, S."Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway". Molecular Medicine Reports 16, no. 1 (2017): 515-522. https://doi.org/10.3892/mmr.2017.6652
Copy and paste a formatted citation
x
Spandidos Publications style
Gao X, Liu Y, Xie Y, Wang Y and Qi S: Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway. Mol Med Rep 16: 515-522, 2017.
APA
Gao, X., Liu, Y., Xie, Y., Wang, Y., & Qi, S. (2017). Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway. Molecular Medicine Reports, 16, 515-522. https://doi.org/10.3892/mmr.2017.6652
MLA
Gao, X., Liu, Y., Xie, Y., Wang, Y., Qi, S."Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway". Molecular Medicine Reports 16.1 (2017): 515-522.
Chicago
Gao, X., Liu, Y., Xie, Y., Wang, Y., Qi, S."Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway". Molecular Medicine Reports 16, no. 1 (2017): 515-522. https://doi.org/10.3892/mmr.2017.6652
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