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Article Open Access

Epigenetically silenced PTPRO functions as a prognostic marker and tumor suppressor in human lung squamous cell carcinoma

  • Authors:
    • Fei Ming
    • Qianqiang Sun
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Hubei Cancer Hospital, Wuhan, Hubei 430000, P.R. China
    Copyright: © Ming et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 746-754
    |
    Published online on: May 31, 2017
       https://doi.org/10.3892/mmr.2017.6665
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Abstract

Protein tyrosine phosphatase receptor‑type O (PTPRO), a member of the PTP family, has been frequently reported as potential tumor suppressor in many types of cancer. However, the exact function of PTPRO in lung squamous cell carcinoma (LSCC) remains unclear. Bisulfite sequencing and methylation specific polymerase chain reaction (PCR) were used to identify the methylation status of PTPRO in LSCC cells, and quantitative methylation specific PCR was used to evaluate the methylation levels of PTPRO in LSCC patients. Stably expressing PTPRO vectors were constructed and transfected into H520 and SK‑MES‑1 cells, followed by MTT and colony formation assays, and analysis of tumor weight and volume in in vivo mouse xenograft models. The present study demonstrated that the CpG island of PTPRO exon 1 was obviously hypermethylated in LSCC cells and tissues. The mRNA expression of PTPRO could be restored by treatment with a demethylation agent. Increased methylation and decreased mRNA levels of PTPRO were observed in LSCC samples compared with adjacent healthy tissues, and were associated with poor prognosis of patients. The mRNA expression of PTPRO was negatively correlated with its methylation level in tumors. Functionally, ectopic PTPRO expression in LSCC cells significantly inhibited the proliferation rates, and colony formation, in comparison with control and non‑transfected cells. In vivo assays confirmed the inhibitory effect of PTPRO on LSCC cell growth. In conclusion, these data provided evidence that epigenetic regulation of PTPRO impairs its tumor suppressor role in LSCC, and restoration of PTPRO may be a potential therapeutic strategy.
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Copy and paste a formatted citation
Spandidos Publications style
Ming F and Sun Q: Epigenetically silenced PTPRO functions as a prognostic marker and tumor suppressor in human lung squamous cell carcinoma. Mol Med Rep 16: 746-754, 2017.
APA
Ming, F., & Sun, Q. (2017). Epigenetically silenced PTPRO functions as a prognostic marker and tumor suppressor in human lung squamous cell carcinoma. Molecular Medicine Reports, 16, 746-754. https://doi.org/10.3892/mmr.2017.6665
MLA
Ming, F., Sun, Q."Epigenetically silenced PTPRO functions as a prognostic marker and tumor suppressor in human lung squamous cell carcinoma". Molecular Medicine Reports 16.1 (2017): 746-754.
Chicago
Ming, F., Sun, Q."Epigenetically silenced PTPRO functions as a prognostic marker and tumor suppressor in human lung squamous cell carcinoma". Molecular Medicine Reports 16, no. 1 (2017): 746-754. https://doi.org/10.3892/mmr.2017.6665
Copy and paste a formatted citation
x
Spandidos Publications style
Ming F and Sun Q: Epigenetically silenced PTPRO functions as a prognostic marker and tumor suppressor in human lung squamous cell carcinoma. Mol Med Rep 16: 746-754, 2017.
APA
Ming, F., & Sun, Q. (2017). Epigenetically silenced PTPRO functions as a prognostic marker and tumor suppressor in human lung squamous cell carcinoma. Molecular Medicine Reports, 16, 746-754. https://doi.org/10.3892/mmr.2017.6665
MLA
Ming, F., Sun, Q."Epigenetically silenced PTPRO functions as a prognostic marker and tumor suppressor in human lung squamous cell carcinoma". Molecular Medicine Reports 16.1 (2017): 746-754.
Chicago
Ming, F., Sun, Q."Epigenetically silenced PTPRO functions as a prognostic marker and tumor suppressor in human lung squamous cell carcinoma". Molecular Medicine Reports 16, no. 1 (2017): 746-754. https://doi.org/10.3892/mmr.2017.6665
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