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Effects of demethoxycurcumin on the viability and apoptosis of skin cancer cells

  • Authors:
    • Yaoqun Wu
    • Pei Zhang
    • Hongyun Yang
    • Yong Ge
    • Yong Xin
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Xiangyang Hospital, Hubei University of Medicine, Xiangyang, Hubei 441000, P.R. China, Department of Radiotherapy, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China, Department of Clinical Laboratory, Xiangyang Central Hospital, Hubei University of Arts and Science, Xiangyang, Hubei 441000, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 539-546
    |
    Published online on: May 31, 2017
       https://doi.org/10.3892/mmr.2017.6666
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Abstract

The present study investigated the effects and mechanisms of demethoxycurcumin (DMC) on a human skin squamous cell carcinoma cell line, A431, and a human keratinocyte cell line, HaCaT. A431 and HaCaT cells were cultured in vitro. The effects of DMC treatment on cell viability were analyzed using the Cell Counting kit‑8 (CCK‑8) assay; cell cycle distribution was analyzed by flow cytometry; apoptosis was assessed by flow cytometry and Hoechst 33258 staining; and the protein expression levels of cytochrome c, B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein (BAX), caspase‑9 and caspase‑3 were evaluated by western blotting. CCK‑8 assay results demonstrated that DMC treatment significantly inhibited viability of A431 and HaCaT cells in a dose‑dependent manner. Flow cytometric analysis confirmed that DMC treatment induced apoptosis in a dose‑dependent manner, and significantly increased the proportion of cells in G2/M phase. Western blot analysis indicated that the protein expression levels of Bcl‑2 were decreased, whereas the expression levels of BAX, caspase‑9, caspase‑3 and cytochrome c were increased following DMC treatment compared with in untreated cells. In conclusion, DMC treatment significantly inhibited viability of A431 and HaCaT cells, and induced cell cycle arrest in G2/M phase. The present study indicated that DMC may induce apoptosis of skin cancer cells through a caspase‑dependent pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Wu Y, Zhang P, Yang H, Ge Y and Xin Y: Effects of demethoxycurcumin on the viability and apoptosis of skin cancer cells. Mol Med Rep 16: 539-546, 2017.
APA
Wu, Y., Zhang, P., Yang, H., Ge, Y., & Xin, Y. (2017). Effects of demethoxycurcumin on the viability and apoptosis of skin cancer cells. Molecular Medicine Reports, 16, 539-546. https://doi.org/10.3892/mmr.2017.6666
MLA
Wu, Y., Zhang, P., Yang, H., Ge, Y., Xin, Y."Effects of demethoxycurcumin on the viability and apoptosis of skin cancer cells". Molecular Medicine Reports 16.1 (2017): 539-546.
Chicago
Wu, Y., Zhang, P., Yang, H., Ge, Y., Xin, Y."Effects of demethoxycurcumin on the viability and apoptosis of skin cancer cells". Molecular Medicine Reports 16, no. 1 (2017): 539-546. https://doi.org/10.3892/mmr.2017.6666
Copy and paste a formatted citation
x
Spandidos Publications style
Wu Y, Zhang P, Yang H, Ge Y and Xin Y: Effects of demethoxycurcumin on the viability and apoptosis of skin cancer cells. Mol Med Rep 16: 539-546, 2017.
APA
Wu, Y., Zhang, P., Yang, H., Ge, Y., & Xin, Y. (2017). Effects of demethoxycurcumin on the viability and apoptosis of skin cancer cells. Molecular Medicine Reports, 16, 539-546. https://doi.org/10.3892/mmr.2017.6666
MLA
Wu, Y., Zhang, P., Yang, H., Ge, Y., Xin, Y."Effects of demethoxycurcumin on the viability and apoptosis of skin cancer cells". Molecular Medicine Reports 16.1 (2017): 539-546.
Chicago
Wu, Y., Zhang, P., Yang, H., Ge, Y., Xin, Y."Effects of demethoxycurcumin on the viability and apoptosis of skin cancer cells". Molecular Medicine Reports 16, no. 1 (2017): 539-546. https://doi.org/10.3892/mmr.2017.6666
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