Inhibitory capacity of Rhus coriaria L. extract and its major component methyl gallate on Streptococcus mutans biofilm formation by optical profilometry: Potential applications for oral health

Kacergius,Tomas; Abu‑Lafi,Saleh; Kirkliauskiene,Agne; Gabe,Vika; Adawi,Azmi; Rayan,Mahmoud; Qutob,Mutaz; Stukas,Rimantas; Utkus,Algirdas; Zeidan,Mouhammad; Rayan,Anwar

doi:10.3892/mmr.2017.6674

July-2017 Volume 16 Issue 1

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

July-2017 Volume 16 Issue 1

Full Size Image

Article

Inhibitory capacity of Rhus coriaria L. extract and its major component methyl gallate on Streptococcus mutans biofilm formation by optical profilometry: Potential applications for oral health

Authors:
- Tomas Kacergius
- Saleh Abu‑Lafi
- Agne Kirkliauskiene
- Vika Gabe
- Azmi Adawi
- Mahmoud Rayan
- Mutaz Qutob
- Rimantas Stukas
- Algirdas Utkus
- Mouhammad Zeidan
- Anwar Rayan
View Affiliations / Copyright

Affiliations: Department of Physiology, Biochemistry, Microbiology and Laboratory Medicine, Faculty of Medicine, Vilnius University, Vilnius 03101, Lithuania, Faculty of Pharmacy, Al‑Quds University, Abu‑Dies 144, Palestine, Institute of Applied Research‑Galilee Society, Shefa‑Amr 20200, Israel, Department of Earth and Environmental Studies, Al‑Quds University, Abu‑Dies 144, Palestine, Institute of Public Health, Faculty of Medicine, Vilnius University, Vilnius 03101, Lithuania, Department of Human and Medical Genetics, Faculty of Medicine, Vilnius University, Vilnius 03101, Lithuania, Drug Discovery Informatics Lab, QRC‑Qasemi Research Center, Al‑Qasemi Academic College, Baka El‑Garbiah 30100, Israel
Pages: 949-956
|
Published online on: June 1, 2017

https://doi.org/10.3892/mmr.2017.6674
Expand metrics +

Abstract

Streptococcus mutans (S. mutans) bacterium is the most well recognized pathogen involved in pathogenesis of dental caries. Its virulence arises from its ability to produce a biofilm and acidogenicity, causing tooth decay. Discovery of natural products capable to inhibit biofilm formation is of high importance for developing health care products. To the best of our knowledge, in all previous scientific reports, a colorimetric assay was applied to test the effect of sumac and methyl gallate (MG) on S. mutans adherence. Quantitative assessment of the developed biofilm should be further performed by applying an optical profilometry assay, and by testing the effect on both surface roughness and thickness parameters of the biofilm. To the best of our knowledge, this is the first study to report the effect of sumac extract and its constituent MG on biofilm formation using an optical profilometry assay. Testing antibacterial activity of the sumac extract and its fractions revealed that MG is the most bioactive component against S. mutans bacteria. It reduced S. mutans biofilm biomass on the polystyrene surface by 68‑93%, whereas 1 mg/ml MG was able to decrease the biofilm roughness and thickness on the glass surface by 99%. MG also prevented a decrease in pH level by 97%. These bioactivities of MG occurred in a dose‑dependent manner and were significant vs. untreated bacteria. The findings are important for the development of novel pharmaceuticals and formulations of natural products and extracts that possess anti‑biofilm activities with primary applications for oral health, and in a broader context, for the treatment of various bacterial infections.

View Figures

View References

1	Klein MI, Hwang G, Santos PH, Campanella OH and Koo H: Streptococcus mutans-derived extracellular matrix in cariogenic oral biofilms. Front Cell Infect Microbiol. 5:102015. View Article : Google Scholar : PubMed/NCBI
2	Quivey RG, Koo H, Lemos J and Kopycka-Kedzierawski DT: Dental caries: General concepts. Oral microbiology and immunology. 2nd. Lamont RJ, Hajishengallis GN and Jenkinson HF: ASM Press; pp. 229–250. 2014
3	Kassebaum NJ, Bernabé E, Dahiya M, Bhandari B, Murray CJ and Marcenes W: Global burden of untreated caries: A systematic review and metaregression. J Dent Res. 94:650–658. 2015. View Article : Google Scholar : PubMed/NCBI
4	Krzyściak W, Jurczak A, Kościelniak D, Bystrowska B and Skalniak A: The virulence of Streptococcus mutans and the ability to form biofilms. Eur J Clin Microbiol Infect Dis. 33:499–515. 2014. View Article : Google Scholar : PubMed/NCBI
5	Koo H, Falsetta ML and Klein MI: The exopolysaccharide matrix: A virulence determinant of cariogenic biofilm. J Dent Res. 92:1065–1073. 2013. View Article : Google Scholar : PubMed/NCBI
6	Guo L, McLean JS, Lux R, He X and Shi W: The well-coordinated linkage between acidogenicity and aciduricity via insoluble glucans on the surface of Streptococcus mutans. Sci Rep. 5:180152015. View Article : Google Scholar : PubMed/NCBI
7	Venkatesan N, Perumal G and Doble M: Bacterial resistance in biofilm-associated bacteria. Future Microbiol. 10:1743–1750. 2015. View Article : Google Scholar : PubMed/NCBI
8	Hoyle BD and Costerton JW: Bacterial resistance to antibiotics: The role of biofilms. Prog Drug Res. 37:91–105. 1991.PubMed/NCBI
9	Gademann K: Copy, edit, and paste: Natural product approaches to biomaterials and neuroengineering. Acc Chem Res. 48:731–739. 2015. View Article : Google Scholar : PubMed/NCBI
10	Cragg GM and Newman DJ: Medicinals for the millennia: The historical record. Ann N Y Acad Sci. 953:3–25. 2001. View Article : Google Scholar : PubMed/NCBI
11	Zaid H, Raiyn J, Nasser A and Rayan A: Physicochemical properties of natural based products versus synthetic chemicals. Open Nutraceuticals J. 3:194–202. 2010. View Article : Google Scholar
12	Awaisheh SS and Ibrahim SA: Screening of antibacterial activity of lactic acid bacteria against different pathogens found in vacuum-packaged meat products. Foodborne Pathog Dis. 6:1125–1132. 2009. View Article : Google Scholar : PubMed/NCBI
13	Kadan S, Rayan M and Rayan A: Anticancer activity of anise (Pimpinella anisum L.) seed extract. Open Nutraceuticals J. 6:1–5. 2013. View Article : Google Scholar
14	Abachi S, Lee S and Rupasinghe HP: Molecular mechanisms of inhibition of streptococcus species by phytochemicals. Molecules. 21:pii: E215. 2016. View Article : Google Scholar : PubMed/NCBI
15	Abu-Reidah IM, Ali-Shtayeh MS, Jamous RM, Arráez-Román D and Segura-Carretero A: HPLC-DAD-ESI-MS/MS screening of bioactive components from Rhus coriaria L. (Sumac) fruits. Food Chem. 166:179–191. 2015. View Article : Google Scholar : PubMed/NCBI
16	Kang MS, Oh JS, Kang IC, Hong SJ and Choi CH: Inhibitory effect of methyl gallate and gallic acid on oral bacteria. J Microbiol. 46:744–750. 2008. View Article : Google Scholar : PubMed/NCBI
17	Singh K, Senadheera DB, Lévesque CM and Cvitkovitch DG: The copYAZ operon functions in copper efflux, biofilm formation, genetic transformation, and stress tolerance in Streptococcus mutans. J Bacteriol. 197:2545–2557. 2015. View Article : Google Scholar : PubMed/NCBI
18	Merritt JH, Kadouri DE and O'Toole GA: Growing and analyzing static biofilms. Curr Protoc Microbiol Chapter. 1:Unit 1B.1. 2005. View Article : Google Scholar
19	Shemesh M, Tam A and Steinberg D: Differential gene expression profiling of Streptococcus mutans cultured under biofilm and planktonic conditions. Microbiology. 153:1307–1317. 2007. View Article : Google Scholar : PubMed/NCBI
20	Shemesh M, Tam A, Aharoni R and Steinberg D: Genetic adaptation of Streptococcus mutans during biofilm formation on different types of surfaces. BMC Microbiol. 10:512010. View Article : Google Scholar : PubMed/NCBI
21	Vahid-Dastjerdi E, Monadi E, Khalighi HR and Torshabi M: Down-regulation of glycosyl transferase genes in Streptococcus mutans by Punica granatum L. flower and Rhus coriaria L. fruit water extracts. Iran J Pharm Res. 15:513–519. 2016.PubMed/NCBI
22	Thimothe J, Bonsi IA, Padilla-Zakour OI and Koo H: Chemical characterization of red wine grape (Vitis vinifera and Vitis interspecific hybrids) and pomace phenolic extracts and their biological activity against Streptococcus mutans. J Agric Food Chem. 55:10200–10207. 2007. View Article : Google Scholar : PubMed/NCBI
23	Choi JG, Mun SH, Chahar HS, Bharaj P, Kang OH, Kim SG, Shin DW and Kwon DY: Methyl gallate from Galla rhois successfully controls clinical isolates of Salmonella infection in both in vitro and in vivo systems. PLoS One. 9:e1026972014. View Article : Google Scholar : PubMed/NCBI
24	Ooshima T, Matsumura M, Hoshino T, Kawabata S, Sobue S and Fujiwara T: Contributions of three glycosyltransferases to sucrose-dependent adherence of Streptococcus mutans. J Dent Res. 80:1672–1677. 2001. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Inhibitory capacity of Rhus coriaria L. extract and its major component methyl gallate on Streptococcus mutans biofilm formation by optical profilometry: Potential applications for oral health

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

Related Articles