Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August-2017 Volume 16 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2017 Volume 16 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Investigation of the neuroprotective effects of a novel synthetic compound via the mitochondrial pathway

  • Authors:
    • Di Wang
    • Shuang Hu
    • Junrong Zhang
    • Qiuyue Li
    • Xinyu Liu
    • Yu Li
  • View Affiliations / Copyright

    Affiliations: Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin 130118, P.R. China, School of Life Sciences, Jilin University, Changchun, Jilin 130012, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1133-1138
    |
    Published online on: June 9, 2017
       https://doi.org/10.3892/mmr.2017.6745
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to investigate the neuroprotective effect of a novel synthetic compound (5zou) on differentiated PC12 cells against 6‑hydroxydopamine (6‑OHDA) and L‑glutamic acid (L‑Glu) neurotoxin‑induced cell injury and the potential mechanisms involved. 5zou is a 2, 2‑disubstituted 1,2‑dihydropyridine. PC12 cells were treated with 6‑OHDA and L‑Glu to establish neurotoxic cell models. MTT assay, DCFH‑DA staining, Fluo‑4‑AM staining, JC‑1 staining and western blotting were used to determine the changes in cell viability, intracellular reactive oxygen species concentration, Ca2+ influx, mitochondrial membrane potential and the protein expressions of B‑cell lymphoma‑2 (Bcl‑2) and B‑cell lymphoma‑extra large (Bcl‑xL). Morphological analysis demonstrated the effect of 5zous on neuritogenesis and differentiation in PC12 cells. The results suggested that 5zou rescued the cell viability, intracellular ROS level, Ca2+ influx, mitochondrial membrane potential, and expression of Bcl‑2 and Bcl‑xL, which were altered by 6‑OHDA and L‑Glu. The study confirmed that 5zou has neuroprotective effects on neurotoxin‑induced differentiated PC12 cells injury, potentially via the mitochondrial apoptosis pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

View References

1 

Amor S, Puentes F, Baker D and van der Valk P: Inflammation in neurodegenerative diseases. Immunology. 129:154–169. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Head E: Combining an antioxidant-fortified diet with behavioral enrichment leads to cognitive improvement and reduced brain pathology in aging canines: Strategies for healthy aging. Ann N Y Acad Sci. 1114:398–406. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Hurny A, Michalowska-Wender G and Wender M: Impact of L-DOPA treatment of patients with Parkinson's disease on mononuclear subsets and phagocytosis in the peripheral blood. Folia Neuropathol. 51:127–131. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Balez R, Steiner N, Engel M, Muñoz SS, Lum JS, Wu Y, Wang D, Vallotton P, Sachdev P, O'Connor M, et al: Neuroprotective effects of apigenin against inflammation, neuronal excitability and apoptosis in an induced pluripotent stem cell model of Alzheimer's disease. Sci Rep. 6:314502016. View Article : Google Scholar : PubMed/NCBI

5 

Zhang J, An S, Hu W, Teng M, Wang X, Qu Y, Liu Y, Yuan Y and Wang D: The neuroprotective properties of hericium erinaceus in glutamate-damaged differentiated PC12 Cells and an alzheimer's disease mouse model. Int J Mol Sci. 17:pii: E18102016. View Article : Google Scholar

6 

Murphy E and Steenbergen C: Mechanisms underlying acute protection from cardiac ischemia-reperfusion injury. Physiol Rev. 88:581–609. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Akopova OV, Kolchynskayia LY, Nosar VY, Smyrnov AN, Malisheva MK, Man'kovskaia YN and Sahach VF: The effect of permeability transition pore opening on reactive oxygen species production in rat brain mitochondria. Ukr Biokhim Zh (1999). 83:46–55. 2011.PubMed/NCBI

8 

Liu CY, Lee CF and Wei YH: Role of reactive oxygen species-elicited apoptosis in the pathophysiology of mitochondrial and neurodegenerative diseases associated with mitochondrial DNA mutations. J Formos Med Assoc. 108:599–611. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Oh KJ, Lee SC, Choi HJ, Oh DY, Kim SC, Min do S, Kim JM, Lee KS and Han JS: Role of phospholipase D2 in anti-apoptotic signaling through increased expressions of Bcl-2 and Bcl-xL. J Cell Biochem. 101:1409–1422. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Su WT and Shih YA: Nanofiber containing carbon nanotubes enhanced PC12 cell proliferation and neuritogenesis by electrical stimulation. Biomed Mater Eng. 26 Suppl 1:S189–S195. 2015.PubMed/NCBI

11 

Jackson TC, Verrier JD and Kochanek PM: Anthraquinone-2-sulfonic acid (AQ2S) is a novel neurotherapeutic agent. Cell Death Dis. 4:e4512013. View Article : Google Scholar : PubMed/NCBI

12 

Kim IS, Koppula S, Kim BW, Song MD, Jung JY, Lee G, Lee HS and Choi DK: A novel synthetic compound PHID (8-Phenyl-6a, 7, 8, 9, 9a, 10-hexahydro-6H-isoindolo [5, 6-g] quinoxaline-7, 9-dione) protects SH-SY5Y cells against MPP(+)-induced cytotoxicity through inhibition of reactive oxygen species generation and JNK signaling. Eur J Pharmacol. 650:48–57. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Zou GF, Hu ZP, Zhang SQ and Liao WW: Synthesis of functionalized 1,2-dihydropyridines bearing quaternary carbon centers via an organocatalytic allylic alkylation. Tetrahedron Lett. 56:937–940. 2015. View Article : Google Scholar

14 

Shimmyo Y, Kihara T, Akaike A, Niidome T and Sugimoto H: Three distinct neuroprotective functions of myricetin against glutamate-induced neuronal cell death: Involvement of direct inhibition of caspase-3. J Neurosci Res. 86:1836–1845. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Yong Y, Ding H, Fan Z, Luo J and Ke ZJ: Lithium fails to protect dopaminergic neurons in the 6-OHDA model of Parkinson's disease. Neurochem Res. 36:367–374. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Garcia-Rivas GJ and Torre-Amione G: Abnormal mitochondrial function during ischemia reperfusion provides targets for pharmacological therapy. Methodist Debakey Cardiovasc J. 5:2–7. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Poyton RO, Ball KA and Castello PR: Mitochondrial generation of free radicals and hypoxic signaling. Trends Endocrinol Metab. 20:332–340. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Kinnally KW and Antonsson B: A tale of two mitochondrial channels, MAC and PTP, in apoptosis. Apoptosis. 12:857–868. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Goitre L, Balzac F, Degani S, Degan P, Marchi S, Pinton P and Retta SF: KRIT1 regulates the homeostasis of intracellular reactive oxygen species. PLoS One. 5:e117862010. View Article : Google Scholar : PubMed/NCBI

20 

Pislar AH, Zidar N, Kikelj D and Kos J: Cathepsin X promotes 6-hydroxydopamine-induced apoptosis of PC12 and SH-SY5Y cells. Neuropharmacology. 82:121–131. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Sun R, Wang K, Wu D, Li X and Ou Y: Protective effect of paeoniflorin against glutamate-induced neurotoxicity in PC12 cells via Bcl-2/Bax signal pathway. Folia Neuropathol. 50:270–276. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Lavik AR, Zhong F, Chang MJ, Greenberg E, Choudhary Y, Smith MR, McColl KS, Pink J, Reu FJ, Matsuyama S and Distelhorst CW: A synthetic peptide targeting the BH4 domain of Bcl-2 induces apoptosis in multiple myeloma and follicular lymphoma cells alone or in combination with agents targeting the BH3-binding pocket of Bcl-2. Oncotarget. 6:27388–27402. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Wang D, Tan QR and Zhang ZJ: Neuroprotective effects of paeoniflorin, but not the isomer albiflorin, are associated with the suppression of intracellular calcium and calcium/calmodulin protein kinase II in PC12 cells. J Mol Neurosci. 51:581–590. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Li W, Cheong YK, Wang H, Ren G and Yang Z: Neuroprotective effects of etidronate and 2,3,3-trisphosphonate against glutamate-induced toxicity in PC12 cells. Neurochem Res. 41:844–854. 2016. View Article : Google Scholar : PubMed/NCBI

25 

Hu S, Wang D, Zhang J, Du M, Cheng Y, Liu Y, Zhang N, Wang D and Wu Y: Mitochondria related pathway is essential for polysaccharides purified from sparassis crispa mediated neuro-protection against glutamate-induced toxicity in differentiated PC12 cells. Int J Mol Sci. 17:pii: E1332016. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang D, Hu S, Zhang J, Li Q, Liu X and Li Y: Investigation of the neuroprotective effects of a novel synthetic compound via the mitochondrial pathway. Mol Med Rep 16: 1133-1138, 2017.
APA
Wang, D., Hu, S., Zhang, J., Li, Q., Liu, X., & Li, Y. (2017). Investigation of the neuroprotective effects of a novel synthetic compound via the mitochondrial pathway. Molecular Medicine Reports, 16, 1133-1138. https://doi.org/10.3892/mmr.2017.6745
MLA
Wang, D., Hu, S., Zhang, J., Li, Q., Liu, X., Li, Y."Investigation of the neuroprotective effects of a novel synthetic compound via the mitochondrial pathway". Molecular Medicine Reports 16.2 (2017): 1133-1138.
Chicago
Wang, D., Hu, S., Zhang, J., Li, Q., Liu, X., Li, Y."Investigation of the neuroprotective effects of a novel synthetic compound via the mitochondrial pathway". Molecular Medicine Reports 16, no. 2 (2017): 1133-1138. https://doi.org/10.3892/mmr.2017.6745
Copy and paste a formatted citation
x
Spandidos Publications style
Wang D, Hu S, Zhang J, Li Q, Liu X and Li Y: Investigation of the neuroprotective effects of a novel synthetic compound via the mitochondrial pathway. Mol Med Rep 16: 1133-1138, 2017.
APA
Wang, D., Hu, S., Zhang, J., Li, Q., Liu, X., & Li, Y. (2017). Investigation of the neuroprotective effects of a novel synthetic compound via the mitochondrial pathway. Molecular Medicine Reports, 16, 1133-1138. https://doi.org/10.3892/mmr.2017.6745
MLA
Wang, D., Hu, S., Zhang, J., Li, Q., Liu, X., Li, Y."Investigation of the neuroprotective effects of a novel synthetic compound via the mitochondrial pathway". Molecular Medicine Reports 16.2 (2017): 1133-1138.
Chicago
Wang, D., Hu, S., Zhang, J., Li, Q., Liu, X., Li, Y."Investigation of the neuroprotective effects of a novel synthetic compound via the mitochondrial pathway". Molecular Medicine Reports 16, no. 2 (2017): 1133-1138. https://doi.org/10.3892/mmr.2017.6745
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team