The role of intestinal endotoxemia in a rat model of aluminum neurotoxicity

Wang,Feng; Guo,Rui‑Xia; Li,Wen‑Xing; Yu,Bao‑Feng; Han,Bai; Liu,Li‑Xin; Han,De‑Wu

doi:10.3892/mmr.2017.6780

The role of intestinal endotoxemia in a rat model of aluminum neurotoxicity

Authors:
- Feng Wang
- Rui‑Xia Guo
- Wen‑Xing Li
- Bao‑Feng Yu
- Bai Han
- Li‑Xin Liu
- De‑Wu Han
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Affiliations: Department of Pathophysiology, Key Laboratory of Cell Physiology of Ministry of Education, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China, Department of Surgery, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030013, P.R. China, Department of Biochemistry and Molecular Biology, Key Laboratory of Cell Physiology of Ministry of Education, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China, Department of Psychiatry, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China, The Experimental Center of Science and Research, The First Hospital, Institute of Hepatopathy, Key Laboratory of Cell Physiology of Ministry of Education, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China
Published online on: June 15, 2017 https://doi.org/10.3892/mmr.2017.6780
Pages: 1878-1884
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the effects of intestinal endotoxemia (IETM) in a rat model of aluminum neurotoxicity established by D-galactose and aluminum trichloride (AlCl3). Adult Wistar rats were administered D‑galactose and AlCl3 to create the aluminum neurotoxicity model. The learning and memory abilities of the rats were subsequently observed using a Morris water maze test and the serum levels of lipopolysaccharide (LPS), tumor necrosis factor (TNF)‑α, interleukin (IL)‑1, diamine oxidase (DAO), glutamine (Gln) and glutaminase were measured. The expression of S‑100β in the serum was detected using an enzyme‑linked immunosorbent assay. The expression levels of the amyloid β‑protein (Aβ) precursor (APP), presenilin 1 (PS1), β‑site APP‑cleaving enzyme (BACE), zona occludens protein (ZO)‑1 and Aβ 1‑40 in the brain of rats were detected via reverse‑transcription polymerase chain reaction, western blotting and immunohistochemistry. The levels of LPS, TNF‑α, IL‑1, DAO, Gln and S‑100β in serum and the mRNA and protein expression levels of APP, PS1, BACE and Aβ1‑40 in the brain were markedly increased in the model rats compared with controls. The level of glutaminase in the serum and the expression of ZO‑1 in the brain were decreased in the model rats compared with controls. IETM was present in the rat model of aluminum neurotoxicity established by D‑galactose and AlCl3 and may be important in the development of this neurotoxicity.

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