Gossypol induces cell death by activating apoptosis and autophagy in HT-29 cells

Lu,Ming‑Dong; Li,Li‑Yi; Li,Pi‑Hong; You,Tao; Wang,Fei‑Hai; Sun,Wei‑Jian; Zheng,Zhi‑Qiang

doi:10.3892/mmr.2017.6804

August-2017 Volume 16 Issue 2

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August-2017 Volume 16 Issue 2

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Article

Gossypol induces cell death by activating apoptosis and autophagy in HT-29 cells

Authors:
- Ming‑Dong Lu
- Li‑Yi Li
- Pi‑Hong Li
- Tao You
- Fei‑Hai Wang
- Wei‑Jian Sun
- Zhi‑Qiang Zheng
View Affiliations / Copyright

Affiliations: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China
Pages: 2128-2132
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Published online on: June 19, 2017

https://doi.org/10.3892/mmr.2017.6804
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Abstract

Gossypol is a polyphenolic, yellowish compound derived from cottonseed extract. The present study examined the effects of gossypol on the apoptosis and autophagy of HT‑29 cells. A Cell Counting Kit‑8 assay, Annexin V‑FITC, JC‑1 staining and western blotting were used to identify the viability of cells, stages of apoptosis and the expression levels of the signaling proteins. Gossypol promoted apoptosis and induced the loss of mitochondrial membrane potential. Further investigation of the apoptotic mechanism revealed that gossypol increased the ratio of B‑cell lymphoma 2 (Bcl-2)-associated X protein/Bcl‑2 protein levels and upregulated the expression of caspase‑3. Gossypol also enhanced the activity of microtubule‑associated protein light chain 3 LC3‑II and Beclin‑1 and downregulated LC3‑I, in a dose‑dependent manner. Together, these finding suggested that gossypol may be a novel and potential antitumor agent.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Gossypol induces cell death by activating apoptosis and autophagy in HT-29 cells

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