Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
September-2017 Volume 16 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2017 Volume 16 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Molecular mechanism and functional role of macrophage colony‑stimulating factor in follicular granulosa cells

  • Authors:
    • Zhang Wei
    • Xu Song
    • Zhang Zhifen
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, Nanjing Medical University, Affiliated Hangzhou Hospital (Hangzhou First People's Hospital), Hangzhou, Zhejiang 310006, P.R. China
  • Pages: 2875-2880
    |
    Published online on: June 27, 2017
       https://doi.org/10.3892/mmr.2017.6857
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Our previous demonstrated that macrophage colony‑stimulating factor (M‑CSF) stimulated the production of estradiol (E2) and progesterone (P) in luteinized granulosa cells (GCs), and that its secretion may be regulated by follicle‑stimulating hormone (FSH). The present study aimed to examine the effect of M‑CSF alone or with Letrozole treatment on the function of non‑luteinizing granulosa cells, using the COV434 cell line, and its interaction with FSH. Human luteinized granulosa cells (LGC) were isolated from the follicular fluid of superovulated infertile patients (average age, 30.8±2.1 years) undergoing an intracytoplasmic sperm injection. The LGC were cultured with various concentrations of recombinant human macrophage colony stimulating factor (rhM‑CSF; 0, 10, 25, 50 or 100 ng/ml), rhM‑CSF+Letrozole (10‑6 mol/l), rhFSH (0, 10, 25, 50 or 100 IU/ml), or rhFSH+Letrozole (10‑6 mol/l). E2 concentrations in the media were measured using ELISA. The expression levels of the FSH receptor and the M‑CSF receptor were detected via reverse transcription‑quantitative polymerase chain reaction. Following COV434 cell treatment with M‑CSF, cell proliferation was quantified using the MTS assay and protein expression was detected by western blotting. It was demonstrated that M‑CSF and FSH stimulated the production of E2. The production of FSH receptors was enhanced by rhM‑CSF or rhM‑CSF+Letrozole in vitro in a dose‑dependent manner. It was observed that rhFSH promoted the expression of the M‑CSF receptor, at a certain concentration. Proliferation of COV434 cells increased in a dose‑dependent manner following treatment with RhM‑CSF. Furthermore, M‑CSF induced the phosphorylation of c‑Jun N‑terminal kinase (JNK) and p38; however, the level of E2 in the medium was not altered when the cells were pretreated with the JNK inhibitor SP600125 or the p38 inhibitor SB203580. The present study suggested that M‑CSF may be important in regulating the response of GCs to gonadotropin and may have a promotive effect in the early phase of follicular development. The biological effects of M‑CSF may partially be attributed to activation of the JNK and p38 signaling pathways. M‑CSF may represent a novel follicular development regulator agent in the future.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Clark SC and Kamen R: The human hematopoietic colony-stimulating factors. J Science. 236:1229–1237. 1987. View Article : Google Scholar

2 

Witt BR and Pollard JW: Colony stimulating factor-1 in human follicular fluid. J Fertil Steril. 68:259–264. 1997. View Article : Google Scholar

3 

Cohen PE, Zhu L, Nishimura K and Pollard JW: Colony-stimulating factor 1 regulation of neuroendocrine pathways that control gonadal function in mice. J Endocrinology. 143:1413–1422. 2002. View Article : Google Scholar

4 

Nishimura K, Tanaka N, Kawano T, Matsuura K and Okamura H: Change in macrophage colony-stimulating factor concentration in serum and follicular fluid in in-vitro fertilization and embryo transfer cycles. J Fertil Steril. 69:53–57. 1998. View Article : Google Scholar

5 

Salmassi A, Mettler L, Jonat W, Buck S, Koch K and Schmutzler AG: Circulating level of macrophage colony-stimulating factor can be predictive for human in vitro fertilization outcome. J Fertil Steril. 93:116–123. 2010. View Article : Google Scholar

6 

Takasaki A, Ohba T, Okamura Y, Honda R, Seki M, Tanaka N and Okamura H: Clinical use of colony-stimulating factor-1 in ovulation induction for poor responders. J Fertil Steril. 90:2287–2290. 2008. View Article : Google Scholar

7 

Zhang Z, Salmassi A and Mettler L: Expression of macrophage colony-stimulating factor in human luteinized granulosa cells. Zhonghua Fu Chan Ke Za Zhi. 37:472–474. 2002.(In Chinese). PubMed/NCBI

8 

Zhang Z, Salmassi A and Mettler L: Effects of macrophage colony-stimulating factor on production of estradiol and progesterone by human luteinized granulosa cells in vitro and detection of macrophage colony-stimulating factor receptor on human luteinized granulosa cells. Zhonghua Fu Chan Ke Za Zhi. 37:338–341. 2002.(In Chinese). PubMed/NCBI

9 

Curry JM, Eubank TD, Roberts RD, Wang Y, Pore N, Maity A and Marsh CB: M-CSF signals through the MAPK/ERK pathway via sp1 to induce VEGF production and induces angiogenesis in vivo. J PLoS One. 3:e34052008. View Article : Google Scholar

10 

Nikolic D, Calderon L, Du L and Post SR: SR-A ligand and M-CSF dynamically regulate SR-A expression and function in primary macrophage via P-38 MAPK activation. J BMC Immunol. 12:372011. View Article : Google Scholar

11 

Strober W: Trypan Blue exclusion test of cell viability. Curr Protoc Immunol: Appendix. 3:Appendix 3B. 2001. View Article : Google Scholar

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Field SL, Dasgupta T, Cummings M and Orsi NM: Cytokines in ovarian folliculogenesis, oocyte maturation and luteinisation. Mol Reprod Dev. 81:284–314. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Ojeda-Ojeda M, Murri M, Insenser M and Escobar-Morreale HF: Mediators of low-grade chronic inflammation in polycystic ovary syndrome (PCOS). Curr Pharm Des. 19:5775–5791. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Ianchiĭ RI, Voznesens'ka TIu and Shepel' OA: Cytokines and their role in reproductive system. Fiziol Zh. 53:82–90. 2007.(in Ukrainian). PubMed/NCBI

16 

Luo J, Elwood F, Britschgi M, Villeda S, Zhang H, Ding Z, Zhu L, Alabsi H, Getachew R, Narasimhan R, et al: Colony-stimulating factor 1 receptor (CSF1R) signaling in injured neurons facilitates protection and survival. J Exp Med. 210:157–172. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Zhang Z, Fang Q and Wang J: Involvement of macrophage colony-stimulating factor (M-CSF) in the function of follicular GCs. Fertil Steril. 90:749–754. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Kawamura K, Chen Y, Shu Y, Cheng Y, Qiao J, Behr B, Pera RA and Hsueh AJ: Promotion of human early embryonic development and blastocyst outgrowth in vitro using autocrine/paracrine growth factors. PLoS One. 7:e493282012. View Article : Google Scholar : PubMed/NCBI

19 

Araki M, Fukumatsu Y, Katabuchi H, Shultz LD, Takahashi K and Okamura H: Follicular development and ovulation in macrophage colony-stimulating factor-deficient mice homozygous for the osteopetrosis (op) mutation. Biol Reprod. 54:478–484. 1996. View Article : Google Scholar : PubMed/NCBI

20 

Sweet MJ and Hume DA: CSF-1 as a regulator of macrophage activation and immune responses. Arch Immunol Ther Exp (Warsz). 51:169–177. 2003.PubMed/NCBI

21 

Chitu V and Stanley ER: Colony-stimulating factor-1 in immunity and inflammation. Curr Opin Immunol. 18:39–48. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Schönlau F, Schlesiger C, Ehrchen J, Grabbe S, Sorg C and Sunderkötter C: Monocyte and macrophage functions in M-CSF-deficient op/op mice during experimental leishmaniasis. J Leukoc Biol. 73:564–573. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Zhang Z, Fang Q and Wang J: Involvement of macrophage colony-stimulating factor (M-CSF) in the function of follicular GCs. Ferti lSteril. 90:749–754. 2008. View Article : Google Scholar

24 

Chakraborty P and Roy SK: Expression of FSH receptor in the hamster ovary during perinatal development. Mol Cell Endocrinol. 400:41–47. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Lee AW, Mao Y, Penninger JM and Yu S: Gab2 promotes colony-stimulating factor 1-regulated macrophage expansion via alternate effectors at different stages of development. Mol Cell Biol. 31:4563–4581. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Popa-Wagner A, Stöcker K, Balseanu AT, Rogalewski A, Diederich K, Minnerup J, Margaritescu C and Schäbitz WR: Effects of granulocyte-colony stimulating factor after stroke in aged rats. Stroke. 41:1027–1031. 2010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wei Z, Song X and Zhifen Z: Molecular mechanism and functional role of macrophage colony‑stimulating factor in follicular granulosa cells. Mol Med Rep 16: 2875-2880, 2017.
APA
Wei, Z., Song, X., & Zhifen, Z. (2017). Molecular mechanism and functional role of macrophage colony‑stimulating factor in follicular granulosa cells. Molecular Medicine Reports, 16, 2875-2880. https://doi.org/10.3892/mmr.2017.6857
MLA
Wei, Z., Song, X., Zhifen, Z."Molecular mechanism and functional role of macrophage colony‑stimulating factor in follicular granulosa cells". Molecular Medicine Reports 16.3 (2017): 2875-2880.
Chicago
Wei, Z., Song, X., Zhifen, Z."Molecular mechanism and functional role of macrophage colony‑stimulating factor in follicular granulosa cells". Molecular Medicine Reports 16, no. 3 (2017): 2875-2880. https://doi.org/10.3892/mmr.2017.6857
Copy and paste a formatted citation
x
Spandidos Publications style
Wei Z, Song X and Zhifen Z: Molecular mechanism and functional role of macrophage colony‑stimulating factor in follicular granulosa cells. Mol Med Rep 16: 2875-2880, 2017.
APA
Wei, Z., Song, X., & Zhifen, Z. (2017). Molecular mechanism and functional role of macrophage colony‑stimulating factor in follicular granulosa cells. Molecular Medicine Reports, 16, 2875-2880. https://doi.org/10.3892/mmr.2017.6857
MLA
Wei, Z., Song, X., Zhifen, Z."Molecular mechanism and functional role of macrophage colony‑stimulating factor in follicular granulosa cells". Molecular Medicine Reports 16.3 (2017): 2875-2880.
Chicago
Wei, Z., Song, X., Zhifen, Z."Molecular mechanism and functional role of macrophage colony‑stimulating factor in follicular granulosa cells". Molecular Medicine Reports 16, no. 3 (2017): 2875-2880. https://doi.org/10.3892/mmr.2017.6857
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team