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Lentivirus‑mediated RIG‑I knockdown relieves cell proliferation inhibition, cell cycle arrest and apoptosis in ATRA‑induced NB4 cells via the AKT‑FOXO3A signaling pathway in vitro

  • Authors:
    • Lei Chen
    • Ya‑Bin Cui
    • Yu‑Ling Si
    • Wei‑Dong Su
    • Xin‑Chao Wang
    • Hua Pang
    • Li‑Jun Qiu
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Tianjin Fourth Central Hospital, Tianjin 300140, P.R. China, Department of Hematology, Tianjin Fourth Central Hospital, Tianjin 300140, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2556-2562
    |
    Published online on: June 27, 2017
       https://doi.org/10.3892/mmr.2017.6858
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Abstract

Retinoic acid inducible gene I (RIG‑I) is upregulated during all‑trans retinoic acid (ATRA)‑induced terminal granulocytic differentiation of NB4 acute promyelocytic leukemia (APL) cells. However, the function and mechanism of RIG‑I in NB4 cells remains to be fully elucidated. In the present study, lentivirus‑mediated RIG‑I‑knockdown was used to investigate the proliferation, cell cycle and apoptotic processes of ATRA‑induced NB4 cells in vitro using an MTT assay and flow cytometry, respectively. The roles of RIG‑I and the AKT‑FOXO3A signaling pathway were investigated using western blot analysis. The results showed that the ATRA‑induced expression of RIG‑I was specifically and effectively knocked down at the mRNA and protein levels by lentivirus mediated RIG‑I short hairpin RNA. In addition, silencing of RIG‑I reduced the ATRA‑induced inhibition of NB4 cell proliferation, cell cycle arrest and apoptosis. Further investigations indicated that with ATRA‑induced expression of RIG‑I, levels of phosphorylated (p)AKT‑Thr308 and pForkhead Box (FOX) O3A‑Thr32 were decreased, the expression levels of cell cycle arrest protein p27 and the apoptotic protein, tumor necrosis factor‑related apoptosis‑inducing ligand (TRAIL), directly transcribed by FOXO3A were increased. By contrast, following the knockdown of ATRA‑induced expression of RIG‑I, the levels of pAKT‑Thr308 and pFOXO3A‑Thr32 were increased, and the protein expression levels of p27 and TRAIL were decreased. Taken together, these results showed that the knockdown of RIG‑I reduced the inhibition of cell proliferation, cell cycle arrest and apoptosis in the ATRA‑induced NB4 cells via the AKT‑FOXO3A signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Chen L, Cui YB, Si YL, Su WD, Wang XC, Pang H and Qiu LJ: Lentivirus‑mediated RIG‑I knockdown relieves cell proliferation inhibition, cell cycle arrest and apoptosis in ATRA‑induced NB4 cells via the AKT‑FOXO3A signaling pathway in vitro. Mol Med Rep 16: 2556-2562, 2017.
APA
Chen, L., Cui, Y., Si, Y., Su, W., Wang, X., Pang, H., & Qiu, L. (2017). Lentivirus‑mediated RIG‑I knockdown relieves cell proliferation inhibition, cell cycle arrest and apoptosis in ATRA‑induced NB4 cells via the AKT‑FOXO3A signaling pathway in vitro. Molecular Medicine Reports, 16, 2556-2562. https://doi.org/10.3892/mmr.2017.6858
MLA
Chen, L., Cui, Y., Si, Y., Su, W., Wang, X., Pang, H., Qiu, L."Lentivirus‑mediated RIG‑I knockdown relieves cell proliferation inhibition, cell cycle arrest and apoptosis in ATRA‑induced NB4 cells via the AKT‑FOXO3A signaling pathway in vitro". Molecular Medicine Reports 16.3 (2017): 2556-2562.
Chicago
Chen, L., Cui, Y., Si, Y., Su, W., Wang, X., Pang, H., Qiu, L."Lentivirus‑mediated RIG‑I knockdown relieves cell proliferation inhibition, cell cycle arrest and apoptosis in ATRA‑induced NB4 cells via the AKT‑FOXO3A signaling pathway in vitro". Molecular Medicine Reports 16, no. 3 (2017): 2556-2562. https://doi.org/10.3892/mmr.2017.6858
Copy and paste a formatted citation
x
Spandidos Publications style
Chen L, Cui YB, Si YL, Su WD, Wang XC, Pang H and Qiu LJ: Lentivirus‑mediated RIG‑I knockdown relieves cell proliferation inhibition, cell cycle arrest and apoptosis in ATRA‑induced NB4 cells via the AKT‑FOXO3A signaling pathway in vitro. Mol Med Rep 16: 2556-2562, 2017.
APA
Chen, L., Cui, Y., Si, Y., Su, W., Wang, X., Pang, H., & Qiu, L. (2017). Lentivirus‑mediated RIG‑I knockdown relieves cell proliferation inhibition, cell cycle arrest and apoptosis in ATRA‑induced NB4 cells via the AKT‑FOXO3A signaling pathway in vitro. Molecular Medicine Reports, 16, 2556-2562. https://doi.org/10.3892/mmr.2017.6858
MLA
Chen, L., Cui, Y., Si, Y., Su, W., Wang, X., Pang, H., Qiu, L."Lentivirus‑mediated RIG‑I knockdown relieves cell proliferation inhibition, cell cycle arrest and apoptosis in ATRA‑induced NB4 cells via the AKT‑FOXO3A signaling pathway in vitro". Molecular Medicine Reports 16.3 (2017): 2556-2562.
Chicago
Chen, L., Cui, Y., Si, Y., Su, W., Wang, X., Pang, H., Qiu, L."Lentivirus‑mediated RIG‑I knockdown relieves cell proliferation inhibition, cell cycle arrest and apoptosis in ATRA‑induced NB4 cells via the AKT‑FOXO3A signaling pathway in vitro". Molecular Medicine Reports 16, no. 3 (2017): 2556-2562. https://doi.org/10.3892/mmr.2017.6858
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