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Oxidative stress regulates mitogen‑activated protein kinases and c‑Jun activation involved in heat stress and lipopolysaccharide‑induced intestinal epithelial cell apoptosis

  • Authors:
    • Yanan Liu
    • Zhenglian Wang
    • Weidang Xie
    • Zhengtao Gu
    • Qiulin Xu
    • Lei Su
  • View Affiliations / Copyright

    Affiliations: Department of Intensive Care Unit, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510120, P.R. China, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510000, P.R. China, Department of Intensive Care Unit, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong 510630; P.R. China, Department of ICU, Key Laboratory of Tropical Zone Trauma Care and Tissue Repair of PLA, General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2579-2587
    |
    Published online on: June 27, 2017
       https://doi.org/10.3892/mmr.2017.6859
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Abstract

Heat stress and gut‑derived endotoxinemia are common causes of multiple organ dysfunction syndrome in heat stroke patients. Evidence has demonstrated that cell apoptosis in the small intestine serves an important role in the pathogenesis of heatstroke, which leads to increased intestinal permeability to endotoxin or lipopolysaccharides (LPS) from the gut entering the circulation. However, little is known about the potential underlying mechanisms mediating heat stress combined with LPS‑induced intestinal epithelial cell apoptosis. In the present study, LPS combined with heat stress induced production of reactive oxygen species (ROS), mitochondrial membrane potential disruption and cell apoptosis, which eventually led to increased intestinal permeability and reduced epithelial resistance in the IEC‑6 cell line. Inductions in ROS, mitochondrial membrane potential disruption and cell apoptosis were detected by using an ROS assay kit, 5,5',6,6'‑tetrachloro‑1,1',3,3'tetraethylbenzimidazo carbocyanine iodide dye kit and annexin V‑fluorescein isothiocyanate apoptosis kit, respectively. The effect of ROS on mitogen activated protein kinases (MAPKs) and c‑Jun activation was investigated using the antioxidant drug, butylated hydroxyanisole (BHA) by western blotting. The results of the present study demonstrated that ROS is essential to activate p38, extracellular signal‑regulated kinase (ERK) and c‑Jun, but not c‑Jun N‑terminal kinase (JNK), in LPS combined with heat stress treated cells. Furthermore, ROS, and activation of p38, JNK and c‑Jun, were revealed to serve pro‑apoptosis roles which aggravated damage to epithelial barrier integrity, as assessed by flow cytometry using Annexin V‑fluorescein isothiocyanate staining and pretreatment of cells with specific inhibitors of ROS, JNK, p38 and c‑Jun (BHA, SP600125, SB203580 and c‑Jun peptide, respectively). Transepithelial electrical resistance and horseradish peroxidase permeability were detected in cells treated with LPS combined with heat stress, which revealed that ERK serves an anti‑apoptosis role, as determined by pretreatment of cells with PD98059, a specific inhibitor of ERK. In conclusion, these findings suggested a novel role of the ROS signaling pathway which involved activation of MAPKs and c‑Jun, following LPS combined with heat stress‑induced IEC‑6 cell apoptosis and impairment of the epithelial barrier. These results may facilitate understanding of pathological conditions involving ROS, such as heat stroke.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Wang Z, Xie W, Gu Z, Xu Q and Su L: Oxidative stress regulates mitogen‑activated protein kinases and c‑Jun activation involved in heat stress and lipopolysaccharide‑induced intestinal epithelial cell apoptosis. Mol Med Rep 16: 2579-2587, 2017.
APA
Liu, Y., Wang, Z., Xie, W., Gu, Z., Xu, Q., & Su, L. (2017). Oxidative stress regulates mitogen‑activated protein kinases and c‑Jun activation involved in heat stress and lipopolysaccharide‑induced intestinal epithelial cell apoptosis. Molecular Medicine Reports, 16, 2579-2587. https://doi.org/10.3892/mmr.2017.6859
MLA
Liu, Y., Wang, Z., Xie, W., Gu, Z., Xu, Q., Su, L."Oxidative stress regulates mitogen‑activated protein kinases and c‑Jun activation involved in heat stress and lipopolysaccharide‑induced intestinal epithelial cell apoptosis". Molecular Medicine Reports 16.3 (2017): 2579-2587.
Chicago
Liu, Y., Wang, Z., Xie, W., Gu, Z., Xu, Q., Su, L."Oxidative stress regulates mitogen‑activated protein kinases and c‑Jun activation involved in heat stress and lipopolysaccharide‑induced intestinal epithelial cell apoptosis". Molecular Medicine Reports 16, no. 3 (2017): 2579-2587. https://doi.org/10.3892/mmr.2017.6859
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Wang Z, Xie W, Gu Z, Xu Q and Su L: Oxidative stress regulates mitogen‑activated protein kinases and c‑Jun activation involved in heat stress and lipopolysaccharide‑induced intestinal epithelial cell apoptosis. Mol Med Rep 16: 2579-2587, 2017.
APA
Liu, Y., Wang, Z., Xie, W., Gu, Z., Xu, Q., & Su, L. (2017). Oxidative stress regulates mitogen‑activated protein kinases and c‑Jun activation involved in heat stress and lipopolysaccharide‑induced intestinal epithelial cell apoptosis. Molecular Medicine Reports, 16, 2579-2587. https://doi.org/10.3892/mmr.2017.6859
MLA
Liu, Y., Wang, Z., Xie, W., Gu, Z., Xu, Q., Su, L."Oxidative stress regulates mitogen‑activated protein kinases and c‑Jun activation involved in heat stress and lipopolysaccharide‑induced intestinal epithelial cell apoptosis". Molecular Medicine Reports 16.3 (2017): 2579-2587.
Chicago
Liu, Y., Wang, Z., Xie, W., Gu, Z., Xu, Q., Su, L."Oxidative stress regulates mitogen‑activated protein kinases and c‑Jun activation involved in heat stress and lipopolysaccharide‑induced intestinal epithelial cell apoptosis". Molecular Medicine Reports 16, no. 3 (2017): 2579-2587. https://doi.org/10.3892/mmr.2017.6859
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