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Article

Resveratrol suppresses thyroid hormone‑induced osteocalcin synthesis in osteoblasts

  • Authors:
    • Kazuhiko Fujita
    • Haruhiko Tokuda
    • Shingo Kainuma
    • Gen Kuroyanagi
    • Naohiro Yamamoto
    • Rie Matsushima‑Nishiwaki
    • Atsushi Harada
    • Osamu Kozawa
    • Takanobu Otsuka
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi 467‑8601, Japan, Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu 501‑1194, Japan, Department of Orthopedic Surgery, National Center for Geriatrics and Gerontology, Obu, Aichi 474‑8511, Japan
  • Pages: 2881-2886
    |
    Published online on: June 28, 2017
       https://doi.org/10.3892/mmr.2017.6872
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Abstract

Resveratrol, a polyphenolic compound that is present in grape skins, berries and red wine, may be beneficial for human health through its anti‑inflammatory and anti‑oxidant effects. It has been previously demonstrated that resveratrol exerts its biological effects primarily via sirtuin 1 (SIRT1) activation. We previously reported that triiodothyronine (T3) induces osteocalcin synthesis in osteoblast‑like MC3T3‑E1 cells, and that p38 mitogen‑activated protein (MAP) kinase mediates the T3‑stimulated synthesis of osteocalcin. The present study investigated the effect of resveratrol on T3‑induced osteocalcin synthesis and its underlying mechanism in MC3T3‑E1 cells. Cultured cells were stimulated with T3, and osteocalcin release from MC3T3‑E1 cells was measured by ELISA and phosphorylation of p38 MAP kinase was analyzed by western blotting. Resveratrol significantly suppressed the release of osteocalcin stimulated by T3, and SRT1720, a SIRT1 activator, significantly reduced T3‑induced osteocalcin release. The expression level of osteocalcin mRNA stimulated by T3 was significantly attenuated by resveratrol and T3‑induced transactivation activity of the thyroid hormone‑responsive element was significantly diminished by resveratrol. However, only limited effects of resveratrol on the T3‑induced phosphorylation of p38 MAP kinase were observed. The results of the present study demonstrated that resveratrol suppresses T3‑stimulated osteocalcin synthesis at a point upstream of transcription in osteoblasts, and that the inhibitory effect of resveratrol is mediated, at least partially, through SIRT1 activation. These results indicate that there may be a novel role for the polyphenol in the modulation of bone metabolism.
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Copy and paste a formatted citation
Spandidos Publications style
Fujita K, Tokuda H, Kainuma S, Kuroyanagi G, Yamamoto N, Matsushima‑Nishiwaki R, Harada A, Kozawa O and Otsuka T: Resveratrol suppresses thyroid hormone‑induced osteocalcin synthesis in osteoblasts. Mol Med Rep 16: 2881-2886, 2017.
APA
Fujita, K., Tokuda, H., Kainuma, S., Kuroyanagi, G., Yamamoto, N., Matsushima‑Nishiwaki, R. ... Otsuka, T. (2017). Resveratrol suppresses thyroid hormone‑induced osteocalcin synthesis in osteoblasts. Molecular Medicine Reports, 16, 2881-2886. https://doi.org/10.3892/mmr.2017.6872
MLA
Fujita, K., Tokuda, H., Kainuma, S., Kuroyanagi, G., Yamamoto, N., Matsushima‑Nishiwaki, R., Harada, A., Kozawa, O., Otsuka, T."Resveratrol suppresses thyroid hormone‑induced osteocalcin synthesis in osteoblasts". Molecular Medicine Reports 16.3 (2017): 2881-2886.
Chicago
Fujita, K., Tokuda, H., Kainuma, S., Kuroyanagi, G., Yamamoto, N., Matsushima‑Nishiwaki, R., Harada, A., Kozawa, O., Otsuka, T."Resveratrol suppresses thyroid hormone‑induced osteocalcin synthesis in osteoblasts". Molecular Medicine Reports 16, no. 3 (2017): 2881-2886. https://doi.org/10.3892/mmr.2017.6872
Copy and paste a formatted citation
x
Spandidos Publications style
Fujita K, Tokuda H, Kainuma S, Kuroyanagi G, Yamamoto N, Matsushima‑Nishiwaki R, Harada A, Kozawa O and Otsuka T: Resveratrol suppresses thyroid hormone‑induced osteocalcin synthesis in osteoblasts. Mol Med Rep 16: 2881-2886, 2017.
APA
Fujita, K., Tokuda, H., Kainuma, S., Kuroyanagi, G., Yamamoto, N., Matsushima‑Nishiwaki, R. ... Otsuka, T. (2017). Resveratrol suppresses thyroid hormone‑induced osteocalcin synthesis in osteoblasts. Molecular Medicine Reports, 16, 2881-2886. https://doi.org/10.3892/mmr.2017.6872
MLA
Fujita, K., Tokuda, H., Kainuma, S., Kuroyanagi, G., Yamamoto, N., Matsushima‑Nishiwaki, R., Harada, A., Kozawa, O., Otsuka, T."Resveratrol suppresses thyroid hormone‑induced osteocalcin synthesis in osteoblasts". Molecular Medicine Reports 16.3 (2017): 2881-2886.
Chicago
Fujita, K., Tokuda, H., Kainuma, S., Kuroyanagi, G., Yamamoto, N., Matsushima‑Nishiwaki, R., Harada, A., Kozawa, O., Otsuka, T."Resveratrol suppresses thyroid hormone‑induced osteocalcin synthesis in osteoblasts". Molecular Medicine Reports 16, no. 3 (2017): 2881-2886. https://doi.org/10.3892/mmr.2017.6872
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