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Article

Sasanquasaponin induces increase of Cl‑/HCO3‑ exchange of anion exchanger 3 and promotes intracellular Cl‑ efflux in hypoxia/reoxygenation cardiomyocytes

  • Authors:
    • Ling‑Yu Qiu
    • Guang-Ling Duan
    • Yu‑Feng Yan
    • Yuan‑Yuan Li
    • Huan Wang
    • Ling Xiao
    • Zhang‑Ping Liao
    • He‑Ping Chen
  • View Affiliations / Copyright

    Affiliations: The Key Laboratory of Basic Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, P.R. China
  • Pages: 2953-2961
    |
    Published online on: June 29, 2017
       https://doi.org/10.3892/mmr.2017.6882
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Abstract

Anion exchanger 3 (AE3) is known to serve crucial roles in maintaining intracellular chloride homeostasis by facilitating the reversible electroneutral exchange of Cl‑ for HCO3‑ across the plasma membrane. Our previous studies reported that sasanquasaponin (SQS) can inhibit hypoxia/reoxygenation (H/R)‑induced elevation of intracellular Cl‑ concentration ([Cl‑]i) and elicit cardioprotection by favoring Cl‑/HCO3‑ exchange of AE3. However, the molecular basis for SQS‑induced increase of Cl‑/HCO3‑ exchange of AE3 remains unclear. The present study demonstrated that SQS activates protein kinase Cε (PKCε) and stimulates the phosphorylation of AE3 in H9c2 cells. Notably, SQS‑induced AE3 phosphorylation was blocked by the PKCε selective inhibitor εV1‑2, and a S67A mutation of AE3, indicating that SQS could promote phosphorylation of Ser67 of AE3 via a PKCε‑dependent regulatory signaling pathway. Additionally, both inhibition of PKCε by εV1‑2 and S67A mutation of AE3 eradicated the SQS‑induced increase of AE3 activity, reversed the inhibitory effect of SQS on H/R‑induced elevation of [Cl‑]i, Ca2+ overload and generation of reactive oxygen species, and eliminated SQS‑induced cardioprotection. In conclusion, PKCε‑dependent phosphorylation of serine 67 on AE3 may be responsible for the increase of Cl‑/HCO3‑ exchange of AE3 and intracellular chloride efflux by SQS, and contributes to the cardioprotection of SQS against H/R in H9c2 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Qiu LY, Duan G, Yan YF, Li YY, Wang H, Xiao L, Liao ZP and Chen HP: Sasanquasaponin induces increase of Cl‑/HCO3‑ exchange of anion exchanger 3 and promotes intracellular Cl‑ efflux in hypoxia/reoxygenation cardiomyocytes. Mol Med Rep 16: 2953-2961, 2017.
APA
Qiu, L., Duan, G., Yan, Y., Li, Y., Wang, H., Xiao, L. ... Chen, H. (2017). Sasanquasaponin induces increase of Cl‑/HCO3‑ exchange of anion exchanger 3 and promotes intracellular Cl‑ efflux in hypoxia/reoxygenation cardiomyocytes. Molecular Medicine Reports, 16, 2953-2961. https://doi.org/10.3892/mmr.2017.6882
MLA
Qiu, L., Duan, G., Yan, Y., Li, Y., Wang, H., Xiao, L., Liao, Z., Chen, H."Sasanquasaponin induces increase of Cl‑/HCO3‑ exchange of anion exchanger 3 and promotes intracellular Cl‑ efflux in hypoxia/reoxygenation cardiomyocytes". Molecular Medicine Reports 16.3 (2017): 2953-2961.
Chicago
Qiu, L., Duan, G., Yan, Y., Li, Y., Wang, H., Xiao, L., Liao, Z., Chen, H."Sasanquasaponin induces increase of Cl‑/HCO3‑ exchange of anion exchanger 3 and promotes intracellular Cl‑ efflux in hypoxia/reoxygenation cardiomyocytes". Molecular Medicine Reports 16, no. 3 (2017): 2953-2961. https://doi.org/10.3892/mmr.2017.6882
Copy and paste a formatted citation
x
Spandidos Publications style
Qiu LY, Duan G, Yan YF, Li YY, Wang H, Xiao L, Liao ZP and Chen HP: Sasanquasaponin induces increase of Cl‑/HCO3‑ exchange of anion exchanger 3 and promotes intracellular Cl‑ efflux in hypoxia/reoxygenation cardiomyocytes. Mol Med Rep 16: 2953-2961, 2017.
APA
Qiu, L., Duan, G., Yan, Y., Li, Y., Wang, H., Xiao, L. ... Chen, H. (2017). Sasanquasaponin induces increase of Cl‑/HCO3‑ exchange of anion exchanger 3 and promotes intracellular Cl‑ efflux in hypoxia/reoxygenation cardiomyocytes. Molecular Medicine Reports, 16, 2953-2961. https://doi.org/10.3892/mmr.2017.6882
MLA
Qiu, L., Duan, G., Yan, Y., Li, Y., Wang, H., Xiao, L., Liao, Z., Chen, H."Sasanquasaponin induces increase of Cl‑/HCO3‑ exchange of anion exchanger 3 and promotes intracellular Cl‑ efflux in hypoxia/reoxygenation cardiomyocytes". Molecular Medicine Reports 16.3 (2017): 2953-2961.
Chicago
Qiu, L., Duan, G., Yan, Y., Li, Y., Wang, H., Xiao, L., Liao, Z., Chen, H."Sasanquasaponin induces increase of Cl‑/HCO3‑ exchange of anion exchanger 3 and promotes intracellular Cl‑ efflux in hypoxia/reoxygenation cardiomyocytes". Molecular Medicine Reports 16, no. 3 (2017): 2953-2961. https://doi.org/10.3892/mmr.2017.6882
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