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Article Open Access

Circulating level of high mobility group box‑1 predicts the severity of community‑acquired pneumonia: Regulation of inflammatory responses via the c‑Jun N‑terminal signaling pathway in macrophages

  • Authors:
    • Hsiang‑Ling Wang
    • Shih‑Ming Tsao
    • Chao‑Bin Yeh
    • Ying‑Erh Chou
    • Shun‑Fa Yang
  • View Affiliations / Copyright

    Affiliations: Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan, R.O.C., Institute of Biochemistry, Microbiology and Immunology, Chung Shan Medical University Hospital, Taichung 402, Taiwan, R.O.C., School of Medicine, Chung Shan Medical University Hospital, Taichung 402, Taiwan, R.O.C.
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2361-2366
    |
    Published online on: June 30, 2017
       https://doi.org/10.3892/mmr.2017.6892
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Abstract

High mobility group box‑1 (HMGB‑1) has been reported to serve significant roles in various inflammatory diseases. However, the correlation between the circulating level of HMGB‑1 and severity of community‑acquired pneumonia (CAP) remains unclear. The present study investigated differential alterations in plasma HMGB‑1 levels of patients with CAP prior to and following antibiotic treatment, and further analyzed the association between CAP severity and HMGB‑1 levels. Furthermore, lipopolysaccharide (LPS)‑induced HMGB‑1 expression in RAW264.7 macrophages and the relevant signaling pathways were examined. Plasma HMGB‑1 levels of 90 patients with CAP and 52 healthy controls were measured using a commercial ELISA. The levels of plasma HMGB‑1 were significantly elevated in CAP patients compared with the controls, and antibiotic treatment was effective in reducing HMGB‑1 levels. Plasma HMGB‑1 correlated with the pneumonia severity index score (r=0.566, P<0.001). Furthermore, LPS‑stimulation significantly upregulated HMGB‑1 secretion via the c‑Jun N‑terminal kinase (JNK) signaling pathway in RAW264.7 macrophages, whereas pretreatment with the JNK inhibitor SP600125 markedly downregulated LPS‑induced HMGB‑1 levels. In conclusion, plasma HMGB‑1 levels may serve a role in the diagnosis and clinical assessment of CAP severity. These findings may provide information on novel targets for the treatment of CAP.
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Copy and paste a formatted citation
Spandidos Publications style
Wang HL, Tsao SM, Yeh CB, Chou YE and Yang SF: Circulating level of high mobility group box‑1 predicts the severity of community‑acquired pneumonia: Regulation of inflammatory responses via the c‑Jun N‑terminal signaling pathway in macrophages. Mol Med Rep 16: 2361-2366, 2017.
APA
Wang, H., Tsao, S., Yeh, C., Chou, Y., & Yang, S. (2017). Circulating level of high mobility group box‑1 predicts the severity of community‑acquired pneumonia: Regulation of inflammatory responses via the c‑Jun N‑terminal signaling pathway in macrophages. Molecular Medicine Reports, 16, 2361-2366. https://doi.org/10.3892/mmr.2017.6892
MLA
Wang, H., Tsao, S., Yeh, C., Chou, Y., Yang, S."Circulating level of high mobility group box‑1 predicts the severity of community‑acquired pneumonia: Regulation of inflammatory responses via the c‑Jun N‑terminal signaling pathway in macrophages". Molecular Medicine Reports 16.3 (2017): 2361-2366.
Chicago
Wang, H., Tsao, S., Yeh, C., Chou, Y., Yang, S."Circulating level of high mobility group box‑1 predicts the severity of community‑acquired pneumonia: Regulation of inflammatory responses via the c‑Jun N‑terminal signaling pathway in macrophages". Molecular Medicine Reports 16, no. 3 (2017): 2361-2366. https://doi.org/10.3892/mmr.2017.6892
Copy and paste a formatted citation
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Spandidos Publications style
Wang HL, Tsao SM, Yeh CB, Chou YE and Yang SF: Circulating level of high mobility group box‑1 predicts the severity of community‑acquired pneumonia: Regulation of inflammatory responses via the c‑Jun N‑terminal signaling pathway in macrophages. Mol Med Rep 16: 2361-2366, 2017.
APA
Wang, H., Tsao, S., Yeh, C., Chou, Y., & Yang, S. (2017). Circulating level of high mobility group box‑1 predicts the severity of community‑acquired pneumonia: Regulation of inflammatory responses via the c‑Jun N‑terminal signaling pathway in macrophages. Molecular Medicine Reports, 16, 2361-2366. https://doi.org/10.3892/mmr.2017.6892
MLA
Wang, H., Tsao, S., Yeh, C., Chou, Y., Yang, S."Circulating level of high mobility group box‑1 predicts the severity of community‑acquired pneumonia: Regulation of inflammatory responses via the c‑Jun N‑terminal signaling pathway in macrophages". Molecular Medicine Reports 16.3 (2017): 2361-2366.
Chicago
Wang, H., Tsao, S., Yeh, C., Chou, Y., Yang, S."Circulating level of high mobility group box‑1 predicts the severity of community‑acquired pneumonia: Regulation of inflammatory responses via the c‑Jun N‑terminal signaling pathway in macrophages". Molecular Medicine Reports 16, no. 3 (2017): 2361-2366. https://doi.org/10.3892/mmr.2017.6892
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