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Peroxisome proliferator‑activated receptor γ mediates porcine placental angiogenesis through hypoxia inducible factor‑, vascular endothelial growth factor‑ and angiopoietin‑mediated signaling

  • Authors:
    • Juzuo Zhang
    • Xuan Peng
    • Anwen Yuan
    • Yang Xie
    • Qing Yang
    • Liqun Xue
  • View Affiliations / Copyright

    Affiliations: Department of Clinic Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan 410128, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2636-2644
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    Published online on: June 30, 2017
       https://doi.org/10.3892/mmr.2017.6903
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Abstract

Peroxisome proliferator-activated receptor (PPAR) γ has been reported to be implicated in placentation in mice. Previous studies have demonstrated that PPARγ is also expressed in porcine placenta, primarily localized in vascular endothelial cells (VECs). The present study aimed to investigate the roles of PPARγ during porcine placental angiogenesis and examine the molecular mechanisms involved in its actions. VECs were incubated with the PPARγ agonist rosiglitazone and the antagonist T0070907, and their angiogenic potential was evaluated using cellular impedance, wound healing and tube formation assays. Reverse transcription‑quantitative polymerase chain reaction was used to assess the mRNA expression levels of angiogenic factors, including hypoxia‑inducible factors (HIFs), vascular endothelial growth factor (VEGF) isoforms, VEGF receptors (VEGFRs) and angiopoietins (Angs). The results demonstrated that the adhesive, proliferative and migratory capabilities of VECs were potentiated by rosiglitazone and suppressed by T0070907. Notably, tube formation was invariably promoted during PPARγ activation and blockade. The mRNA expression levels of HIF1α, HIF2α, VEGFR2, VEGF188 and Ang‑1 were revealed to be upregulated following treatment of VECs with rosiglitazone, whereas they were downregulated following treatment with T0070907. However, the mRNA expression levels of placental growth factor and VEGF120 were consistently downregulated following PPARγ activation and blockade, whereas VEGF164 mRNA levels remained unaltered. The results of the present study suggested that PPARγ may mediate porcine placental angiogenesis, by interfering with HIF‑, VEGF‑ and angiopoietin‑mediated signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Peng X, Yuan A, Xie Y, Yang Q and Xue L: Peroxisome proliferator‑activated receptor γ mediates porcine placental angiogenesis through hypoxia inducible factor‑, vascular endothelial growth factor‑ and angiopoietin‑mediated signaling. Mol Med Rep 16: 2636-2644, 2017.
APA
Zhang, J., Peng, X., Yuan, A., Xie, Y., Yang, Q., & Xue, L. (2017). Peroxisome proliferator‑activated receptor γ mediates porcine placental angiogenesis through hypoxia inducible factor‑, vascular endothelial growth factor‑ and angiopoietin‑mediated signaling. Molecular Medicine Reports, 16, 2636-2644. https://doi.org/10.3892/mmr.2017.6903
MLA
Zhang, J., Peng, X., Yuan, A., Xie, Y., Yang, Q., Xue, L."Peroxisome proliferator‑activated receptor γ mediates porcine placental angiogenesis through hypoxia inducible factor‑, vascular endothelial growth factor‑ and angiopoietin‑mediated signaling". Molecular Medicine Reports 16.3 (2017): 2636-2644.
Chicago
Zhang, J., Peng, X., Yuan, A., Xie, Y., Yang, Q., Xue, L."Peroxisome proliferator‑activated receptor γ mediates porcine placental angiogenesis through hypoxia inducible factor‑, vascular endothelial growth factor‑ and angiopoietin‑mediated signaling". Molecular Medicine Reports 16, no. 3 (2017): 2636-2644. https://doi.org/10.3892/mmr.2017.6903
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Peng X, Yuan A, Xie Y, Yang Q and Xue L: Peroxisome proliferator‑activated receptor γ mediates porcine placental angiogenesis through hypoxia inducible factor‑, vascular endothelial growth factor‑ and angiopoietin‑mediated signaling. Mol Med Rep 16: 2636-2644, 2017.
APA
Zhang, J., Peng, X., Yuan, A., Xie, Y., Yang, Q., & Xue, L. (2017). Peroxisome proliferator‑activated receptor γ mediates porcine placental angiogenesis through hypoxia inducible factor‑, vascular endothelial growth factor‑ and angiopoietin‑mediated signaling. Molecular Medicine Reports, 16, 2636-2644. https://doi.org/10.3892/mmr.2017.6903
MLA
Zhang, J., Peng, X., Yuan, A., Xie, Y., Yang, Q., Xue, L."Peroxisome proliferator‑activated receptor γ mediates porcine placental angiogenesis through hypoxia inducible factor‑, vascular endothelial growth factor‑ and angiopoietin‑mediated signaling". Molecular Medicine Reports 16.3 (2017): 2636-2644.
Chicago
Zhang, J., Peng, X., Yuan, A., Xie, Y., Yang, Q., Xue, L."Peroxisome proliferator‑activated receptor γ mediates porcine placental angiogenesis through hypoxia inducible factor‑, vascular endothelial growth factor‑ and angiopoietin‑mediated signaling". Molecular Medicine Reports 16, no. 3 (2017): 2636-2644. https://doi.org/10.3892/mmr.2017.6903
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