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Article

General regulatory effects of hypoxia on human cartilage endplate‑derived stem cells: A genome‑wide analysis of differential gene expression and alternative splicing events

  • Authors:
    • Yuan Yao
    • Weilin Song
    • Qiyue Deng
    • Huiyu Zhang
    • Jian Wang
    • Huan Liu
    • Yue Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P.R. China, Department of Ophthalmology, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R. China, Department of Neurobiology, College of Basic Medical Sciences, Third Military Medical University, Chongqing 400038, P.R. China, Department of Stomatology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P.R. China
  • Pages: 3001-3009
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    Published online on: June 30, 2017
       https://doi.org/10.3892/mmr.2017.6907
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Abstract

Intervertebral disc (IVD) degeneration of is considered to be initiated by the degeneration of the cartilage endplate (CEP). CEP‑derived stem cells (CESCs) with the capacity for osteochondrogenic differentiation may be responsible for CEP cartilage restoration. As CEP is avascular and hypoxic, and hypoxia can greatly influence biological activities of stem cells, physiological hypoxia may serve important roles in regulating the physiological functions of CESCs. The aim of the present study was to investigate the mechanisms of hypoxia‑regulated CESCs fate by using the Human Transcriptome Array 2.0 system to identify differentially expressed genes (DEGs) and alternatively spliced genes (ASGs) in CESCs cultured under hypoxic and normoxic conditions. The high‑throughput analysis of both DEGs and ASGs were notably enriched in the immune response signal, which so far has not been investigated in IVD cells, due to their avascular nature and low immunogenicity. The present results provided a referential study direction of the mechanisms of hypoxia‑regulated CESC fate at the level of gene expression and alternative splicing, which may aid in our understanding of the processes of CEP degeneration.
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1 

Andersson GB: Epidemiological features of chronic low-back pain. Lancet. 354:581–585. 1999. View Article : Google Scholar : PubMed/NCBI

2 

Le Maitre CL, Freemont AJ and Hoyland JA: Accelerated cellular senescence in degenerate intervertebral discs: A possible role in the pathogenesis of intervertebral disc degeneration. Arthritis Res Ther. 9:R452007. View Article : Google Scholar : PubMed/NCBI

3 

Peng B, Hao J, Hou S, Wu W, Jiang D, Fu X and Yang Y: Possible pathogenesis of painful intervertebral disc degeneration. Spine (Phila Pa 1976). 31:560–566. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Wang AM, Cao P, Yee A, Chan D and Wu EX: Detection of extracellular matrix degradation in intervertebral disc degeneration by diffusion magnetic resonance spectroscopy. Magn Reson Med. 73:1703–1712. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Buckwalter JA: Aging and degeneration of the human intervertebral disc. Spine (Phila Pa 1976). 20:1307–1314. 1995. View Article : Google Scholar : PubMed/NCBI

6 

Holm S, Maroudas A, Urban JP, Selstam G and Nachemson A: Nutrition of the intervertebral disc: Solute transport and metabolism. Connect Tissue Res. 8:101–119. 1981. View Article : Google Scholar : PubMed/NCBI

7 

Raj PP: Intervertebral disc: Anatomy-physiology-pathophysiology-treatment. Pain Pract. 8:18–44. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Li FC, Zhang N, Chen WS and Chen QX: Endplate degeneration may be the origination of the vacuum phenomenon in intervertebral discs. Med Hypotheses. 75:169–171. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Liu LT, Huang B, Li CQ, Zhuang Y, Wang J and Zhou Y: Characteristics of stem cells derived from the degenerated human intervertebral disc cartilage endplate. PLoS One. 6:e262852011. View Article : Google Scholar : PubMed/NCBI

10 

Boskey AL: Signaling in response to hypoxia and normoxia in the intervertebral disc. Arthritis Rheum. 58:3637–3639. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Carmeliet P, Dor Y, Herbert JM, Fukumura D, Brusselmans K, Dewerchin M, Neeman M, Bono F, Abramovitch R, Maxwell P, et al: Role of HIF-1alpha in hypoxia-mediated apoptosis, cell proliferation and tumour angiogenesis. Nature. 394:485–490. 1998. View Article : Google Scholar : PubMed/NCBI

12 

Merceron C, Vinatier C, Portron S, Masson M, Amiaud J, Guigand L, Chérel Y, Weiss P and Guicheux J: Differential effects of hypoxia on osteochondrogenic potential of human adipose-derived stem cells. Am J Physiol Cell Physiol. 298:C355–C364. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Makino Y, Kanopka A, Wilson WJ, Tanaka H and Poellinger L: Inhibitory PAS domain protein (IPAS) is a hypoxia-inducible splicing variant of the hypoxia-inducible factor-3alpha locus. J Biol Chem. 277:32405–32408. 2002. View Article : Google Scholar : PubMed/NCBI

14 

Tacconelli A, Farina AR, Cappabianca L, Desantis G, Tessitore A, Vetuschi A, Sferra R, Rucci N, Argenti B, Screpanti I, et al: TrkA alternative splicing: A regulated tumor-promoting switch in human neuroblastoma. Cancer Cell. 6:347–360. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Kazantseva J, Kivil A, Tints K, Kazantseva A, Neuman T and Palm K: Alternative splicing targeting the hTAF4-TAFH domain of TAF4 represses proliferation and accelerates chondrogenic differentiation of human mesenchymal stem cells. PLoS One. 8:e747992013. View Article : Google Scholar : PubMed/NCBI

16 

Gabut M, Samavarchi-Tehrani P, Wang X, Slobodeniuc V, O'Hanlon D, Sung HK, Alvarez M, Talukder S, Pan Q, Mazzoni EO, et al: An alternative splicing switch regulates embryonic stem cell pluripotency and reprogramming. Cell. 147:132–146. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Hang X, Li P, Li Z, Qu W, Yu Y, Li H, Shen Z, Zheng H, Gao Y, Wu Y, et al: Transcription and splicing regulation in human umbilical vein endothelial cells under hypoxic stress conditions by exon array. BMC Genomics. 10:1262009. View Article : Google Scholar : PubMed/NCBI

18 

Moller-Levet CS, Betts GN, Harris AL, Homer JJ, West CM and Miller CJ: Exon array analysis of head and neck cancers identifies a hypoxia related splice variant of LAMA3 associated with a poor prognosis. PLoS Comput Biol. 5:e10005712009. View Article : Google Scholar : PubMed/NCBI

19 

Shang J, Fan X, Shangguan L, Liu H and Zhou Y: Global Gene expression profiling and alternative splicing events during the chondrogenic differentiation of human cartilage endplate-derived stem cells. Biomed Res Int. 2015:6049722015. View Article : Google Scholar : PubMed/NCBI

20 

Yao Y, Shang J, Song W, Deng Q, Liu H and Zhou Y: Global profiling of the gene expression and alternative splicing events during hypoxia-regulated chondrogenic differentiation in human cartilage endplate-derived stem cells. Genomics. 107:170–177. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Lee DC, Adams CS, Albert TJ, Shapiro IM, Evans SM and Koch CJ: In situ oxygen utilization in the rat intervertebral disc. J Anat. 210:294–303. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Nerlich AG, Schaaf R, Wälchli B and Boos N: Temporo-spatial distribution of blood vessels in human lumbar intervertebral discs. Eur Spine J. 16:547–555. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Freemont AJ, Watkins A, Le Maitre C, Baird P, Jeziorska M, Knight MT, Ross ER, O'Brien JP and Hoyland JA: Nerve growth factor expression and innervation of the painful intervertebral disc. J Pathol. 197:286–292. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Walsh DA, McWilliams DF, Turley MJ, Dixon MR, Fransès RE, Mapp PI and Wilson D: Angiogenesis and nerve growth factor at the osteochondral junction in rheumatoid arthritis and osteoarthritis. Rheumatology (Oxford). 49:1852–1861. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Amorin B, Alegretti AP, Valim VS, Silva AM, Silva MA, Sehn F and Silla L: Characteristics of mesenchymal stem cells under hypoxia. Cell Bio. 2:11–19. 2013.

27 

Schiller ZA, Schiele NR, Sims JK, Lee K and Kuo CK: Adipogenesis of adipose-derived stem cells may be regulated via the cytoskeleton at physiological oxygen levels in vitro. Stem Cell Res Ther. 4:792013. View Article : Google Scholar : PubMed/NCBI

28 

Redshaw Z and Loughna PT: Oxygen concentration modulates the differentiation of muscle stem cells toward myogenic and adipogenic fates. Differentiation. 84:193–202. 2012. View Article : Google Scholar : PubMed/NCBI

29 

D'Ippolito G, Diabira S, Howard GA, Roos BA and Schiller PC: Low oxygen tension inhibits osteogenic differentiation and enhances stemness of human MIAMI cells. Bone. 39:513–522. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Dos Santos F, Andrade PZ, Boura JS, Abecasis MM, da Silva CL and Cabral JM: Ex vivo expansion of human mesenchymal stem cells: A more effective cell proliferation kinetics and metabolism under hypoxia. J Cell Physiol. 223:27–35. 2010.PubMed/NCBI

31 

Potier E, Ferreira E, Meunier A, Sedel L, Logeart-Avramoglou D and Petite H: Prolonged hypoxia concomitant with serum deprivation induces massive human mesenchymal stem cell death. Tissue Eng. 13:1325–1331. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Kobayashi K, Hernandez LD, Galán JE, Janeway CA Jr, Medzhitov R and Flavell RA: IRAK-M is a negative regulator of Toll-like receptor signaling. Cell. 110:191–202. 2002. View Article : Google Scholar : PubMed/NCBI

33 

Fernandes-Alnemri T, Kang S, Anderson C, Sagara J, Fitzgerald KA and Alnemri ES: Cutting edge: TLR signaling licenses IRAK1 for rapid activation of the NLRP3 inflammasome. J Immunol. 191:3995–3999. 2013. View Article : Google Scholar : PubMed/NCBI

34 

Cohen P: The TLR and IL-1 signalling network at a glance. J Cell Sci. 127:2383–2390. 2014. View Article : Google Scholar : PubMed/NCBI

35 

Sprong T, Roos D, Weemaes C, Neeleman C, Geesing CL, Mollnes TE and van Deuren M: Deficient alternative complement pathway activation due to factor D deficiency by 2 novel mutations in the complement factor D gene in a family with meningococcal infections. Blood. 107:4865–4870. 2006. View Article : Google Scholar : PubMed/NCBI

36 

Rohrer B, Guo Y, Kunchithapautham K and Gilkeson GS: Eliminating complement factor D reduces photoreceptor susceptibility to light-induced damage. Invest Ophthalmol Vis Sci. 48:5282–5289. 2007. View Article : Google Scholar : PubMed/NCBI

37 

Anderson DH, Radeke MJ, Gallo NB, Chapin EA, Johnson PT, Curletti CR, Hancox LS, Hu J, Ebright JN, Malek G, et al: The pivotal role of the complement system in aging and age-related macular degeneration: Hypothesis re-visited. Prog Retin Eye Res. 29:95–112. 2010. View Article : Google Scholar : PubMed/NCBI

38 

Zeng J, Chen Y, Tong Z, Zhou X, Zhao C, Wang K, Hughes G, Kasuga D, Bedell M, Lee C, et al: Lack of association of CFD polymorphisms with advanced age-related macular degeneration. Mol Vis. 16:2273–2278. 2010.PubMed/NCBI

39 

Schraufstatter IU, Khaldoyanidi SK and DiScipio RG: Complement activation in the context of stem cells and tissue repair. World J Stem Cells. 7:1090–1108. 2015. View Article : Google Scholar : PubMed/NCBI

40 

Lee DS, Yi TG, Lee HJ, Kim SN, Park S, Jeon MS and Song SU: Mesenchymal stem cells infected with Mycoplasma arginini secrete complement C3 to regulate immunoglobulin production in B lymphocytes. Cell Death Dis. 5:e11922014. View Article : Google Scholar : PubMed/NCBI

41 

Soland MA, Bego M, Colletti E, Zanjani ED, St Jeor S, Porada CD and Almeida-Porada G: Mesenchymal stem cells engineered to inhibit complement-mediated damage. PLoS One. 8:e604612013. View Article : Google Scholar : PubMed/NCBI

42 

Collard CD, Väkevä A, Morrissey MA, Agah A, Rollins SA, Reenstra WR, Buras JA, Meri S and Stahl GL: Complement activation after oxidative stress: Role of the lectin complement pathway. Am J Pathol. 156:1549–1556. 2000. View Article : Google Scholar : PubMed/NCBI

43 

Cowell RM, Plane JM and Silverstein FS: Complement activation contributes to hypoxic-ischemic brain injury in neonatal rats. J Neurosci. 23:9459–9468. 2003.PubMed/NCBI

44 

Olszowski T, Poziomkowska-Gęsicka I, Jensenius JC and Adler G: Lectin pathway of complement activation in a Polish woman with MASP-2 deficiency. Immunobiology. 219:261–262. 2014. View Article : Google Scholar : PubMed/NCBI

45 

Almeida AR, Arroz-Madeira S, Fonseca-Pereira D, Ribeiro H, Lasrado R, Pachnis V and Veiga-Fernandes H: RET/GFRα signals are dispensable for thymic T cell development in vivo. PloS One. 7:e529492012. View Article : Google Scholar : PubMed/NCBI

46 

Vargas-Leal V, Bruno R, Derfuss T, Krumbholz M, Hohlfeld R and Meinl E: Expression and function of glial cell line-derived neurotrophic factor family ligands and their receptors on human immune cells. J Immunol. 175:2301–2308. 2005. View Article : Google Scholar : PubMed/NCBI

47 

Liu CF and Lefebvre V: The transcription factors SOX9 and SOX5/SOX6 cooperate genome-wide through super-enhancers to drive chondrogenesis. Nucleic Acids Res. 43:8183–8203. 2015. View Article : Google Scholar : PubMed/NCBI

48 

Choung HW, Lee DS, Lee HK, Shon WJ and Park JC: Preameloblast-derived factors mediate osteoblast differentiation of human bone marrow mesenchymal stem cells by Runx2-Osterix-BSP signaling. Tissue Eng Part A. 22:93–102. 2015. View Article : Google Scholar : PubMed/NCBI

49 

Haddad JJ: Endotoxin-mediated regulation of nuclear factor-kappaB nuclear translocation and activation in the hippocampus of the central nervous system: Modulation by intracerebroventricular treatment with thymulin and the immunomodulatory role of the IkappaB-alpha/pIkappaB-alpha pathway. Neuroscience. 164:1509–1520. 2009. View Article : Google Scholar : PubMed/NCBI

50 

Nguyen TL Xuan, Choi JW, Lee SB, Ye K, Woo SD, Lee KH and Ahn JY: Akt phosphorylation is essential for nuclear translocation and retention in NGF-stimulated PC12 cells. Biochem Biophys Res Commun. 349:789–798. 2006. View Article : Google Scholar : PubMed/NCBI

51 

Mathieu PS and Loboa EG: Cytoskeletal and focal adhesion influences on mesenchymal stem cell shape, mechanical properties and differentiation down osteogenic, adipogenic and chondrogenic pathways. Tissue Eng Part B Rev. 18:436–444. 2012. View Article : Google Scholar : PubMed/NCBI

52 

Lee HJ, Jang M, Kim H, Kwak W, Park W, Hwang JY, Lee CK, Jang GW, Park MN, Kim HC, et al: Comparative transcriptome analysis of adipose tissues reveals that ECM-Receptor interaction is involved in the depot-specific adipogenesis in cattle. PLoS One. 8:e662672013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yao Y, Song W, Deng Q, Zhang H, Wang J, Liu H and Zhou Y: General regulatory effects of hypoxia on human cartilage endplate‑derived stem cells: A genome‑wide analysis of differential gene expression and alternative splicing events. Mol Med Rep 16: 3001-3009, 2017.
APA
Yao, Y., Song, W., Deng, Q., Zhang, H., Wang, J., Liu, H., & Zhou, Y. (2017). General regulatory effects of hypoxia on human cartilage endplate‑derived stem cells: A genome‑wide analysis of differential gene expression and alternative splicing events. Molecular Medicine Reports, 16, 3001-3009. https://doi.org/10.3892/mmr.2017.6907
MLA
Yao, Y., Song, W., Deng, Q., Zhang, H., Wang, J., Liu, H., Zhou, Y."General regulatory effects of hypoxia on human cartilage endplate‑derived stem cells: A genome‑wide analysis of differential gene expression and alternative splicing events". Molecular Medicine Reports 16.3 (2017): 3001-3009.
Chicago
Yao, Y., Song, W., Deng, Q., Zhang, H., Wang, J., Liu, H., Zhou, Y."General regulatory effects of hypoxia on human cartilage endplate‑derived stem cells: A genome‑wide analysis of differential gene expression and alternative splicing events". Molecular Medicine Reports 16, no. 3 (2017): 3001-3009. https://doi.org/10.3892/mmr.2017.6907
Copy and paste a formatted citation
x
Spandidos Publications style
Yao Y, Song W, Deng Q, Zhang H, Wang J, Liu H and Zhou Y: General regulatory effects of hypoxia on human cartilage endplate‑derived stem cells: A genome‑wide analysis of differential gene expression and alternative splicing events. Mol Med Rep 16: 3001-3009, 2017.
APA
Yao, Y., Song, W., Deng, Q., Zhang, H., Wang, J., Liu, H., & Zhou, Y. (2017). General regulatory effects of hypoxia on human cartilage endplate‑derived stem cells: A genome‑wide analysis of differential gene expression and alternative splicing events. Molecular Medicine Reports, 16, 3001-3009. https://doi.org/10.3892/mmr.2017.6907
MLA
Yao, Y., Song, W., Deng, Q., Zhang, H., Wang, J., Liu, H., Zhou, Y."General regulatory effects of hypoxia on human cartilage endplate‑derived stem cells: A genome‑wide analysis of differential gene expression and alternative splicing events". Molecular Medicine Reports 16.3 (2017): 3001-3009.
Chicago
Yao, Y., Song, W., Deng, Q., Zhang, H., Wang, J., Liu, H., Zhou, Y."General regulatory effects of hypoxia on human cartilage endplate‑derived stem cells: A genome‑wide analysis of differential gene expression and alternative splicing events". Molecular Medicine Reports 16, no. 3 (2017): 3001-3009. https://doi.org/10.3892/mmr.2017.6907
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