Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
September-2017 Volume 16 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2017 Volume 16 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Serum is an indispensable factor in the maintenance of the biological characteristics of sweat gland cells

  • Authors:
    • Qing Sun
    • Xiao‑Mei Deng
    • Yun‑Liang Wang
    • Yun‑Fang Zhen
    • Fang Li
    • Rui‑Hua Chen
    • Han‑Si Liang
    • Feng Zhang
    • Ming‑De Qin
    • Xue‑Guang Zhang
  • View Affiliations / Copyright

    Affiliations: Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215007, P.R. China, The Stem Cell and Biomedical Material Key Laboratory of Jiangsu, Soochow University, Suzhou, Jiangsu 215007, P.R. China, Department of General Surgery, The First Affiliated Hospital of Soochow University, Soochow University, Suzhou, Jiangsu 215007, P.R. China, Institute of Pediatrics, Children's Hospital of Soochow University, Suzhou, Jiangsu 215005, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2691-2699
    |
    Published online on: July 4, 2017
       https://doi.org/10.3892/mmr.2017.6909
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The tolerance of sweat gland cells for in vitro amplification and subcultivation is low as they are somatic cells. The present study aimed to formulate an optimal medium for the culture of human eccrine sweat gland cells (HESGCs) and to establish a method for induction of HESGCs proliferation, whilst maintaining the characteristics of sweat gland cells. HESGCs cultured in sweat gland (SG):keratinocyte growth medium‑2 (KGM‑2) (1:1) medium had a higher proliferation rate and a stable morphology compared with cells cultured in SG and KGM‑2 medium only. Reverse transcription‑quantitative polymerase chain reaction indicated that cells cultured in the SG:KGM‑2 (1:1) medium exhibited higher expression levels of α‑smooth muscle actin, keratin (K)77, carcinoembryonic antigen, K8, K18, ectodysplasin A receptor, c‑Myc, Kruppel‑like factor 4 and octamer‑binding transcription factor 4 compared with cells cultured in SG only or KGM‑2 only medium. Three‑dimensional culture analysis revealed that HESGCs cultured in SG:KGM‑2 1:1 medium differentiated into sweat gland‑like structures, whereas cells cultured in KGM‑2 only medium underwent cornification. The present study also determined that the maintenance of the biological characteristics of HESGCs occurred due to the presence of fetal bovine serum (FBS). Cells cultured in medium without FBS differentiated into keratinocytes. Therefore, the SG:KGM‑2 (1:1) medium may be a suitable culture medium for HESGCs. In conclusion, this mixed medium is a valuable compound and should be considered to be a potential supplemental medium for HESGCs.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Cui CY and Schlessinger D: Eccrine sweat gland development and sweat secretion. Exp Dermatol. 24:644–650. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Dobson RL and Sato K: The secretion of salt and water by the eccrine weat gland. Arch Dermatol. 105:366–370. 1972. View Article : Google Scholar : PubMed/NCBI

3 

Gallico GG III, O'Connor NE, Compton CC, Kehinde O and Green H: Permanent coverage of large burn wounds with autologous cultured human epithelium. N Engl J Med. 311:448–451. 1984. View Article : Google Scholar : PubMed/NCBI

4 

Danner S, Kremer M, Petschnik AE, Nagel S, Zhang Z, Hopfner U, ReckhenrichG AK, Weber C, Schenck TL, Becker T, et al: The use of human sweat gland-derived stem cells for enhancing vascularization during dermal regeneration. J Invest Dermatol. 132:1707–1716. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Pontiggia L, Biedermann T, Böttcher-Haberzeth S, Oliveira C, Braziulis E, Klar AS, Meuli-Simmen C, Meuli M and Reichmann E: De novo epidermal regeneration using human eccrine sweat gland cells: Higher competence of secretory over absorptive cells. J Invest Dermatol. 134:1735–1742. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Li HH, Zhou G, Fu XB and Zhang L: Antigen expression of human eccrine sweat glands. J Cutan Pathol. 36:318–324. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Li HH, Fu XB, Zhang L and Zhou G: Comparison of proliferating cells between human adult and fetal eccrine sweat glands. Arch Dermatol Res. 300:173–176. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Hagmann S, Moradi B, Frank S, Dreher T, Kämmerer PW, Richter W and Gotterbarm T: Different culture media affect growth characteristics, surface marker distribution and chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells. BMC Musculoskelet Disord. 14:2232013. View Article : Google Scholar : PubMed/NCBI

9 

Wang Y, Liu ZY, Zhao Q, Sun TZ, Ma K and Fu XB: Future application of hair follicle stem cells: Capable in differentiation into sweat gland cells. Chin Med J (Engl). 126:3545–3552. 2013.PubMed/NCBI

10 

Böttcher-Haberzeth S, Biedermann T, Pontiggia L, Braziulis E, Schiestl C, Hendriks B, Eichhoff OM, Widmer DS, Meuli-Simmen C, Meuli M and Reichmann E: Human eccrine sweat gland cells turn into melanin-uptaking keratinocytes in dermo-epidermal skin substitutes. J Invest Dermatol. 133:316–324. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Schön M, Benwood J, O'Connell-Willstaedt T and Rheinwald JG: Human sweat gland myoepithelial cells express a unique set of cytokeratins and reveal the potential for alternative epithelial and mesenchymal differentiation states in culture. J Cell Sci. 112:1925–1936. 1999.PubMed/NCBI

12 

Czifra G, Szöllősi AG, Tóth BI, Demaude J, Bouez C, Breton L and Bíró T: Endocannabinoids regulate growth and survival of human eccrine sweat gland-derived epithelial cells. J Invest Dermatol. 132:1967–1976. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Lee CM, Carpenter F, Coaker T and Kealey T: The primary culture of epithelia from the secretory coil and collecting duct of normal human and cystic fibrotic eccrine sweat glands. J Cell Sci. 83:103–118. 1986.PubMed/NCBI

14 

Biedermann T, Pontiggia L, Böttcher-Haberzeth S, Tharakan S, Braziulis E, Schiestl C, Meuli M and Reichmann E: Human eccrine sweat gland cells can reconstitute a stratified epidermis. J Invest Dermatol. 130:1996–2009. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Lei X, Liu B, Wu J, Lu Y and Yang Y: Matrigel-induced tubular morphogenesis of human eccrine sweat gland epithelial cells. Anat Rec (Hoboken). 294:1525–1531. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Tao R, Han Y, Chai J, Li D and Sun T: Isolation, culture, and verification of human sweat gland epithelial cells. Cytotechnology. 62:489–495. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Lei X, Wu J, Lu Y and Zhu T: Effects of acetylcholine chloride on intracellular calcium concentration of cultured sweat gland epithelial cells. Arch Dermatol Res. 300:335–341. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Uchida N, Oura H, Nakanishi H, Urano Y and Arase S: Dispersed cell culture of human sweat duct cells under serum-free conditions. J Dermatol. 20:684–690. 1993. View Article : Google Scholar : PubMed/NCBI

19 

Collie G, Buchwald M, Harper P and Riordan JR: Culture of sweat gland epithelial cells from normal individuals and patients with cystic fibrosis. In vitro Cell Dev Boil. 21:597–602. 1985. View Article : Google Scholar

20 

Guenou H, Nissan X, Larcher F, Feteira J, Lemaitre G, Saidani M, Del Rio M, Barrault CC, Bernard FX, Peschanski M, et al: Human embryonic stem-cell derivatives for full reconstruction of the pluristratified epidermis: a preclinical study. Lancet. 374:1745–1753. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Sun Q, Li F, Li H, Chen RH, Gu YZ, Chen Y, Liang HS, You XR, Ding SS, Gao L, et al: Amniotic fluid stem cells provide considerable advantages in epidermal regeneration: B7H4 creates a moderate inflammation microenvironment to promote wound repair. Sci Rep. 5:115602015. View Article : Google Scholar : PubMed/NCBI

22 

Lu C and Fuchs E: Sweat gland progenitors in development, homeostasis, and wound repair. Cold Spring Harb Perspect Med. 4:pii: a015222. 2014. View Article : Google Scholar

23 

Morimoto Y and Saga K: Proliferating cells in human eccrine and apocrine sweat glands. J Histochem Cytochem. 43:1217–1221. 1995. View Article : Google Scholar : PubMed/NCBI

24 

Hainfellner JA, Voigtländer T, Ströbel T, Mazal PR, Maddalena AS, Aguzzi A and Budka H: Fibroblasts can express glial fibrillary acidic protein (GFAP) in vivo. J Neuropathol Exp Neurol. 60:449–461. 2001. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Sun Q, Deng XM, Wang YL, Zhen YF, Li F, Chen RH, Liang HS, Zhang F, Qin MD, Zhang XG, Zhang XG, et al: Serum is an indispensable factor in the maintenance of the biological characteristics of sweat gland cells. Mol Med Rep 16: 2691-2699, 2017.
APA
Sun, Q., Deng, X., Wang, Y., Zhen, Y., Li, F., Chen, R. ... Zhang, X. (2017). Serum is an indispensable factor in the maintenance of the biological characteristics of sweat gland cells. Molecular Medicine Reports, 16, 2691-2699. https://doi.org/10.3892/mmr.2017.6909
MLA
Sun, Q., Deng, X., Wang, Y., Zhen, Y., Li, F., Chen, R., Liang, H., Zhang, F., Qin, M., Zhang, X."Serum is an indispensable factor in the maintenance of the biological characteristics of sweat gland cells". Molecular Medicine Reports 16.3 (2017): 2691-2699.
Chicago
Sun, Q., Deng, X., Wang, Y., Zhen, Y., Li, F., Chen, R., Liang, H., Zhang, F., Qin, M., Zhang, X."Serum is an indispensable factor in the maintenance of the biological characteristics of sweat gland cells". Molecular Medicine Reports 16, no. 3 (2017): 2691-2699. https://doi.org/10.3892/mmr.2017.6909
Copy and paste a formatted citation
x
Spandidos Publications style
Sun Q, Deng XM, Wang YL, Zhen YF, Li F, Chen RH, Liang HS, Zhang F, Qin MD, Zhang XG, Zhang XG, et al: Serum is an indispensable factor in the maintenance of the biological characteristics of sweat gland cells. Mol Med Rep 16: 2691-2699, 2017.
APA
Sun, Q., Deng, X., Wang, Y., Zhen, Y., Li, F., Chen, R. ... Zhang, X. (2017). Serum is an indispensable factor in the maintenance of the biological characteristics of sweat gland cells. Molecular Medicine Reports, 16, 2691-2699. https://doi.org/10.3892/mmr.2017.6909
MLA
Sun, Q., Deng, X., Wang, Y., Zhen, Y., Li, F., Chen, R., Liang, H., Zhang, F., Qin, M., Zhang, X."Serum is an indispensable factor in the maintenance of the biological characteristics of sweat gland cells". Molecular Medicine Reports 16.3 (2017): 2691-2699.
Chicago
Sun, Q., Deng, X., Wang, Y., Zhen, Y., Li, F., Chen, R., Liang, H., Zhang, F., Qin, M., Zhang, X."Serum is an indispensable factor in the maintenance of the biological characteristics of sweat gland cells". Molecular Medicine Reports 16, no. 3 (2017): 2691-2699. https://doi.org/10.3892/mmr.2017.6909
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team