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Lysyl oxidase‑like 2 is expressed in kidney tissue and is associated with the progression of tubulointerstitial fibrosis

  • Authors:
    • Sung‑Eun Choi
    • Nara Jeon
    • Hoon Young Choi
    • Jae Il Shin
    • Hyeon Joo Jeong
    • Beom Jin Lim
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Yonsei University College of Medicine, Seoul 03722, Republic of Korea, Department of Internal Medicine, Yonsei University College of Medicine, Seoul 03722, Republic of Korea, Institute of Kidney Disease Research, Yonsei University College of Medicine, Seoul 03722, Republic of Korea
    Copyright: © Choi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2477-2482
    |
    Published online on: July 4, 2017
       https://doi.org/10.3892/mmr.2017.6918
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Abstract

Tubulointerstitial fibrosis is a common end point of chronic kidney diseases, and preventing its progression is key to avoiding renal failure. Transforming growth factor‑β (TGF‑β) and associated molecules promote tubulointerstitial fibrosis; however, effective therapies targeting these molecules have yet to be developed. Lysyl oxidase‑like 2 (LOXL2), which is involved in invasive growth and metastasis of malignant neoplasms, has recently been reported to serve a key role in hepatic and pulmonary fibrosis. However, little is currently known regarding LOXL2 expression in the kidney and its involvement in tubulointerstitial fibrosis. The present study evaluated LOXL2 expression in human and mouse kidney tissues, as well as in cultured renal cells. LOXL2 protein expression was detected in glomerular capillary loops and tubular epithelial cells in human and mouse kidneys. Glomerular LOXL2 was localized to the cytoplasm of podocytes, as determined by double immunofluorescence microscopy using a podocyte marker (synaptopodin). This result was supported by western blot analysis, which demonstrated that LOXL2 protein expression is present in cultured human podocytes and HK‑2 human proximal tubular cells. In addition, the mRNA and protein expression levels of LOXL2 were higher in a mouse model of tubulointerstitial fibrosis compared with in control mice. In addition, immunohistochemistry results demonstrated that LOXL2 is present in the fibrous interstitium and infiltrating mononuclear cells in a mouse model of tubulointerstitial fibrosis. The present study demonstrated that LOXL2 is expressed in compartments of renal tissue, where it appears to contribute to the progression of tubulointerstitial fibrosis.
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Copy and paste a formatted citation
Spandidos Publications style
Choi SE, Jeon N, Choi H, Shin J, Jeong H and Lim B: Lysyl oxidase‑like 2 is expressed in kidney tissue and is associated with the progression of tubulointerstitial fibrosis. Mol Med Rep 16: 2477-2482, 2017.
APA
Choi, S., Jeon, N., Choi, H., Shin, J., Jeong, H., & Lim, B. (2017). Lysyl oxidase‑like 2 is expressed in kidney tissue and is associated with the progression of tubulointerstitial fibrosis. Molecular Medicine Reports, 16, 2477-2482. https://doi.org/10.3892/mmr.2017.6918
MLA
Choi, S., Jeon, N., Choi, H., Shin, J., Jeong, H., Lim, B."Lysyl oxidase‑like 2 is expressed in kidney tissue and is associated with the progression of tubulointerstitial fibrosis". Molecular Medicine Reports 16.3 (2017): 2477-2482.
Chicago
Choi, S., Jeon, N., Choi, H., Shin, J., Jeong, H., Lim, B."Lysyl oxidase‑like 2 is expressed in kidney tissue and is associated with the progression of tubulointerstitial fibrosis". Molecular Medicine Reports 16, no. 3 (2017): 2477-2482. https://doi.org/10.3892/mmr.2017.6918
Copy and paste a formatted citation
x
Spandidos Publications style
Choi SE, Jeon N, Choi H, Shin J, Jeong H and Lim B: Lysyl oxidase‑like 2 is expressed in kidney tissue and is associated with the progression of tubulointerstitial fibrosis. Mol Med Rep 16: 2477-2482, 2017.
APA
Choi, S., Jeon, N., Choi, H., Shin, J., Jeong, H., & Lim, B. (2017). Lysyl oxidase‑like 2 is expressed in kidney tissue and is associated with the progression of tubulointerstitial fibrosis. Molecular Medicine Reports, 16, 2477-2482. https://doi.org/10.3892/mmr.2017.6918
MLA
Choi, S., Jeon, N., Choi, H., Shin, J., Jeong, H., Lim, B."Lysyl oxidase‑like 2 is expressed in kidney tissue and is associated with the progression of tubulointerstitial fibrosis". Molecular Medicine Reports 16.3 (2017): 2477-2482.
Chicago
Choi, S., Jeon, N., Choi, H., Shin, J., Jeong, H., Lim, B."Lysyl oxidase‑like 2 is expressed in kidney tissue and is associated with the progression of tubulointerstitial fibrosis". Molecular Medicine Reports 16, no. 3 (2017): 2477-2482. https://doi.org/10.3892/mmr.2017.6918
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