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Endothelial growth medium suppresses apoptosis of mesenchymal stem cells in vitro via decrease of miR‑29a

  • Authors:
    • Qianqian Wu
    • Tao Fang
    • Min Chen
    • Guoxian Qi
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology of Aging, Department of Cardiology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Orthopedic Surgery, The Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2675-2681
    |
    Published online on: July 6, 2017
       https://doi.org/10.3892/mmr.2017.6939
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Abstract

The administration of mesenchymal stem cells (MSCs) in cases of cardiac ischemia/reperfusion injury (IRI) has been associated with a significant reduction of myocardial cell death and an effective improvement in cardiac function. However, one major limiting factor in MSCs transplantation therapy is the low survival rate of the transplanted cells. The present study aimed to demonstrate that human amnion‑derived mesenchymal stem cells (hAMSCs) cultured with endothelial growth medium (EGM‑2) exhibited reduced apoptosis when exposed to serum‑free and hypoxic conditions; and that the expression of microRNA (miR)‑29a decreased significantly. Furthermore, miR‑29a knockdown resulted in decreased apoptosis of hAMSCs and increased myeloid cell leukemia (MCL)‑1 at the mRNA and protein levels. These results suggested that EGM‑2 promoted survival of hAMSCs partly through the regulation of miR‑29a and MCL‑1 expression levels. These findings may provide a novel understanding of a potential effective therapeutic strategy for cardiac IRI.
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1 

Parolini O, Alviano F, Bagnara GP, Bilic G, Bühring HJ, Evangelista M, Hennerbichler S, Liu B, Magatti M, Mao N, et al: Concise review: Isolation and characterization of cells from human term placenta: Outcome of the first international workshop on placenta derived stem cells. Stem cells. 26:300–311. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Bollini S, Pozzobon M, Nobles M, Riegler J, Dong X, Piccoli M, Chiavegato A, Price AN, Ghionzoli M, Cheung KK, et al: In vitro and in vivo cardiomyogenic differentiation of amniotic fluid stem cells. Stem Cell Rev. 7:364–380. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Kim SW, Zhang HZ, Kim CE, Kim JM and Kim MH: Amniotic mesenchymal stem cells with robust chemotactic properties are effective in the treatment of a myocardial infarction model. Int J Cardiol. 168:1062–1069. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Katritsis DG, Sotiropoulou PA, Karvouni E, Karabinos I, Korovesis S, Perez SA, Voridis EM and Papamichail M: Transcoronary transplantation of autologous mesenchymal stem cells and endothelial progenitors into infarcted human myocardium. Catheter Cardiovasc Interv. 65:321–329. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Jeevanantham V, Butler M, Saad A, Abdel-Latif A, Zuba-Surma EK and Dawn B: Adult bone marrow cell therapy improves survival and induces long-term improvement in cardiac parameters: A systematic review and meta-analysis. Circulation. 126:551–568. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Hristov M, Heussen N, Schober A and Weber C: Intracoronary infusion of autologous bone marrow cells and left ventricular function after acute myocardial infarction: A meta-analysis. J Cell Mol Med. 10:727–733. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Toma C, Pittenger MF, Cahill KS, Byrne BJ and Kessler PD: Human mesenchymal stem cells differentiate to a cardiomyocyte phenotype in the adult murine heart. Circulation. 105:93–98. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Hua P, Liu JY, Tao J and Yang SR: Application and progress of combined mesenchymal stem cell transplantation in the treatment of ischemic cardiomyopathy. Biomed Res Int. 2015:5685022015. View Article : Google Scholar : PubMed/NCBI

9 

Geng YJ: Molecular mechanisms for cardiovascular stem cell apoptosis and growth in the hearts with atherosclerotic coronary disease and ischemic heart failure. Ann N Y Acad Sci. 1010:687–697. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Hamedi-Asl P, Halabian R, Bahmani P, Mohammadipour M, Mohammadzadeh M, Roushandeh AM, Jahanian-Najafabadi A, Kuwahara Y and Roudkenar MH: Adenovirus-mediated expression of the HO-1 protein within MSCs decreased cytotoxicity and inhibited apoptosis induced by oxidative stresses. Cell Stress Chaperones. 17:181–190. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Tang YL, Tang Y, Zhang YC, Qian K, Shen L and Phillips MI: Improved graft mesenchymal stem cell survival in ischemic heart with a hypoxia-regulated heme oxygenase-1 vector. J Am Coll Cardiol. 46:1339–1350. 2005. View Article : Google Scholar : PubMed/NCBI

12 

He J, Wang C, Sun Y, Lu B, Cui J, Dong N, Zhang M, Liu Y and Yu B: Exendin-4 protects bone marrow-derived mesenchymal stem cells against oxygen/glucose and serum deprivation-induced apoptosis through the activation of the cAMP/PKA signaling pathway and the attenuation of ER stress. Int J Mol Med. 37:889–900. 2016.PubMed/NCBI

13 

Jin J, Jeong SI, Shin YM, Lim KS, Shin Hs, Lee YM, Koh HC and Kim KS: Transplantation of mesenchymal stem cells within a poly (lactide-co-epsilon-caprolactone) scaffold improves cardiac function in a rat myocardial infarction model. Eur J Heart Fail. 11:147–153. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Ye Y, Hu Z, Lin Y, Zhang C and Perez-Polo JR: Downregulation of microRNA-29 by antisense inhibitors and a PPAR-gamma agonist protects against myocardial ischaemia-reperfusion injury. Cardiovasc Res. 87:535–544. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Zhang F, Cui J, Liu X, Lv B, Liu X, Xie Z and Yu B: Roles of microRNA-34a targeting SIRT1 in mesenchymal stem cells. Stem Cell Res Ther. 6:1952015. View Article : Google Scholar : PubMed/NCBI

17 

Xu C, Lu Y, Pan Z, Chu W, Luo X, Lin H, Xiao J, Shan H, Wang Z and Yang B: The muscle-specific microRNAs miR-1 and miR-133 produce opposing effects on apoptosis by targeting HSP60, HSP70 and caspase-9 in cardiomyocytes. J Cell Sci. 120:3045–3052. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Liu X, Wang Z, Wang R, Zhao F, Shi P, Jiang Y and Pang X: Direct comparison of the potency of human mesenchymal stem cells derived from amnion tissue, bone marrow and adipose tissue at inducing dermal fibroblast responses to cutaneous wounds. Int J Mol Med. 31:407–415. 2013.PubMed/NCBI

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Zhu W, Chen J, Cong X, Hu S and Chen X: Hypoxia and serum deprivation-induced apoptosis in mesenchymal stem cells. Stem cells. 24:416–425. 2006. View Article : Google Scholar : PubMed/NCBI

21 

König J, Huppertz B, Desoye G, Parolini O, Fröhlich JD, Weiss G, Dohr G, Sedlmayr P and Lang I: Amnion-derived mesenchymal stromal cells show angiogenic properties but resist differentiation into mature endothelial cells. Stem Cells Dev. 21:1309–1320. 2012. View Article : Google Scholar : PubMed/NCBI

22 

König J, Weiss G, Rossi D, Wankhammer K, Reinisch A, Kinzer M, Huppertz B, Pfeiffer D, Parolini O and Lang I: Placental mesenchymal stromal cells derived from blood vessels or avascular tissues: What is the better choice to support endothelial cell function? Stem Cells Dev. 24:115–131. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Opferman JT, Letai A, Beard C, Sorcinelli MD, Ong CC and Korsmeyer SJ: Development and maintenance of B and T lymphocytes requires antiapoptotic MCL-1. Nature. 426:671–676. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Maurer U, Charvet C, Wagman AS, Dejardin E and Green DR: Glycogen synthase kinase-3 regulates mitochondrial outer membrane permeabilization and apoptosis by destabilization of MCL-1. Mol Cell. 21:749–760. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Mott JL, Kobayashi S, Bronk SF and Gores GJ: Mir-29 regulates Mcl-1 protein expression and apoptosis. Oncogene. 26:6133–6140. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Garzon R, Heaphy CE, Havelange V, Fabbri M, Volinia S, Tsao T, Zanesi N, Kornblau SM, Marcucci G, Calin GA, et al: MicroRNA 29b functions in acute myeloid leukemia. Blood. 114:5331–5341. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Xiong Y, Fang JH, Yun JP, Yang J, Zhang Y, Jia WH and Zhuang SM: Effects of microRNA-29 on apoptosis, tumorigenicity and prognosis of hepatocellular carcinoma. Hepatology. 51:836–845. 2010.PubMed/NCBI

28 

Lin CL, Lee PH, Hsu YC, Lei CC, Ko JY, Chuang PC, Huang YT, Wang SY, Wu SL, Chen YS, et al: MicroRNA-29a promotion of nephrin acetylation ameliorates hyperglycemia-induced podocyte dysfunction. J Am Soc Nephrol. 25:1698–1709. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Roshan R, Shridhar S, Sarangdhar MA, Banik A, Chawla M, Garg M, Singh VP and Pillai B: Brain-specific knockdown of miR-29 results in neuronal cell death and ataxia in mice. RNA. 20:1287–1297. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wu Q, Fang T, Chen M and Qi G: Endothelial growth medium suppresses apoptosis of mesenchymal stem cells in vitro via decrease of miR‑29a. Mol Med Rep 16: 2675-2681, 2017.
APA
Wu, Q., Fang, T., Chen, M., & Qi, G. (2017). Endothelial growth medium suppresses apoptosis of mesenchymal stem cells in vitro via decrease of miR‑29a. Molecular Medicine Reports, 16, 2675-2681. https://doi.org/10.3892/mmr.2017.6939
MLA
Wu, Q., Fang, T., Chen, M., Qi, G."Endothelial growth medium suppresses apoptosis of mesenchymal stem cells in vitro via decrease of miR‑29a". Molecular Medicine Reports 16.3 (2017): 2675-2681.
Chicago
Wu, Q., Fang, T., Chen, M., Qi, G."Endothelial growth medium suppresses apoptosis of mesenchymal stem cells in vitro via decrease of miR‑29a". Molecular Medicine Reports 16, no. 3 (2017): 2675-2681. https://doi.org/10.3892/mmr.2017.6939
Copy and paste a formatted citation
x
Spandidos Publications style
Wu Q, Fang T, Chen M and Qi G: Endothelial growth medium suppresses apoptosis of mesenchymal stem cells in vitro via decrease of miR‑29a. Mol Med Rep 16: 2675-2681, 2017.
APA
Wu, Q., Fang, T., Chen, M., & Qi, G. (2017). Endothelial growth medium suppresses apoptosis of mesenchymal stem cells in vitro via decrease of miR‑29a. Molecular Medicine Reports, 16, 2675-2681. https://doi.org/10.3892/mmr.2017.6939
MLA
Wu, Q., Fang, T., Chen, M., Qi, G."Endothelial growth medium suppresses apoptosis of mesenchymal stem cells in vitro via decrease of miR‑29a". Molecular Medicine Reports 16.3 (2017): 2675-2681.
Chicago
Wu, Q., Fang, T., Chen, M., Qi, G."Endothelial growth medium suppresses apoptosis of mesenchymal stem cells in vitro via decrease of miR‑29a". Molecular Medicine Reports 16, no. 3 (2017): 2675-2681. https://doi.org/10.3892/mmr.2017.6939
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