Involvement of Notch2 in all‑trans retinoic acid‑induced inhibition of mouse embryonic palate mesenchymal cell proliferation

Zhang,Yadong; Dong,Shiyi; Wang,Jianning; Wang,Miao; Chen,Mu; Huang,Hongzhang

doi:10.3892/mmr.2017.6940

September-2017 Volume 16 Issue 3

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September-2017 Volume 16 Issue 3

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Article Open Access

Involvement of Notch2 in all‑trans retinoic acid‑induced inhibition of mouse embryonic palate mesenchymal cell proliferation

Authors:
- Yadong Zhang
- Shiyi Dong
- Jianning Wang
- Miao Wang
- Mu Chen
- Hongzhang Huang
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Affiliations: Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat‑sen University, Guangzhou, Guangdong 510055, P.R. China, Department of Oral and Maxillofacial Surgery, Kiangwu Hospital, Macao, SAR, P.R. China, Department of Stomatology, Nanshan Affiliated Hospital of Guangdong Medical College, Shenzhen, Guangdong 518000, P.R. China

Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 2538-2546
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Published online on: July 6, 2017

https://doi.org/10.3892/mmr.2017.6940
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Abstract

Cleft palate is among the most common congenital disorders, and can be induced by exposure to all‑trans retinoic acid (atRA) during mice and human embryogenesis. However, the mechanism underlying the implication of atRA in the development of cleft palate has yet to be elucidated. In the present study, atRA administered by gavage resulted in formation of a cleft palate in 99% of treated C57BL/6 mice. Notch2 was revealed to be upregulated in mouse embryonic palate mesenchymal (MEPM) cells in the atRA‑treated group compared with untreated control mice between embryonic day (E)12.5 and E14.5. In addition, atRA was demonstrated to mediate Notch2 expression via the activation of RA receptors (RARs). Since Notch2 activation has previously been reported to inhibit the proliferation of MEPM cells, the expression levels of extracellular signal‑regulated kinase (ERK), p21, cyclin D1 and Ki‑67 were assessed in samples from atRA‑treated and control mouse embryos between E12.5 and E14.5. It was demonstrated that Notch2 silencing partially reversed the atRA‑induced inhibition of ERK phosphorylation in MEPM cells. In addition, the atRA‑induced cyclin D1 downregulation and p21 upregulation were partially reversed following Notch2 silencing, whereas the atRA‑induced inhibition of cellular proliferation was also attenuated. Furthermore, it was revealed that Notch2 expression was upregulated, whereas Ki‑67 expression was downregulated following atRA exposure, as assessed using resin bead‑released atRA in MEPM cells. The present findings suggested that during embryonic development, atRA may enhance the expression of Notch2, which may inhibit cellular proliferation, possibly through ERK signaling.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

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Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Involvement of Notch2 in all‑trans retinoic acid‑induced inhibition of mouse embryonic palate mesenchymal cell proliferation

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