miRNA‑1284 inhibits cell growth and induces apoptosis of lung cancer cells

Introduction

Lung cancer is the leading cause of cancer-associated mortality with a consistently poor 5-year survival rate (1). Despite improvements in monitoring and clinical treatment strategies, the 5-year overall survival rate in the United States remains ~16% (2). In 2012, lung cancer occurred in 1.8 million people and resulted in 1.6 million mortalities worldwide (3). Unfortunately, its total incidence is increasing dramatically worldwide (4,5). Most clinical cases of lung cancer are categorized into two subtypes: Small-cell lung carcinoma (SCLC) and non-small-cell lung carcinoma (NSCLC). Approximately 70% of patients newly diagnosed with either subtype of lung cancer present with locally recurrent or metastatic lesions following resection (6). Undoubtedly, elucidating the pathogenesis of lung cancer is important for the effective treatment of this disease.

MicroRNAs (miRNAs) are a class of short, endogenous, noncoding RNAs that regulate gene expression. Although miRNAs account for only a minor fraction of the expressed genome, they have been demonstrated to have important roles in various cellular processes, including proliferation, apoptosis, differentiation and malignant transformation (7–9). It is estimated that 60% genes are regulated by miRNAs, and changes in miRNA expression affect physiological and pathological processes (10). Increasing evidence indicates that miRNAs have critical functions in the initiation and progression of various malignancies, including lung cancer (11,12). For instance, miRNA-145 (miR-145), miR-328, miR-378 and miR-21 have been reported to have important roles in lung cancer (13–15). Recently, miR-1284 was reported to be downregulated in gastric cancer, and miR-1284 overexpression promoted cell cycle arrested at the G0/G1 phase, accelerated drug-induced apoptosis, and decreased migration and invasiveness (16). To the best of our knowledge, the role of miR-1284 in lung cancer has not been previously investigated.

The present study aimed to investigate the role of miR-1284 in lung cancer. Therefore, A549 lung carcinoma cells were transfected with miR-1284 mimic or miR-1284 inhibitor. Cell viability, growth and apoptosis were then investigated under the two conditions. In order to further investigate the underlying molecular mechanisms, the protein expressions of cell cycle regulators (p27 and p21), apoptosis-associated proteins, including Bcl-2 associated X, apoptosis regulator (Bax), pro-caspase-3 and activated caspase-3, and Myc, were determined by western blot analysis. Results of the present study may lead to new treatment options for lung cancer.

Materials and methods

Cell culture

A549 human lung cancer cells were obtained from the American Type Culture Collection (Manassas, VA, USA) and cultured in Dulbecco's modified Eagle's medium (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (Invitrogen; Thermo Fisher Scientific, Inc.) at 37°C and 5% CO2. The miR-1284 mimic (sense, UCU AUA CAG ACC CUG GCU UUU C; antisense, AAA GCC AGG GUC UGU AUA GAU U), miR-1284 inhibitor (GAA AAG CCA GGG UCU GUA UAG A) were synthesized by Shanghai GenePharma Co., Ltd. (Shanghai, China). The scramble miRNA control used was a 22 nucleotide random sequence with a non-targeting RNA duplex (GE Dharmacon; GE Healthcare Life Sciences, Little Chalfont, UK). The cells were transfected with 50 nM miR-1284 mimic, miR-1284 inhibitor or scramble control by using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's manual. Cells were incubated at 37°C in a CO2 incubator for 24–48 h.

RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR)

At 48 h after transfection was complete, total RNA (5×106 cells) was extracted by TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.) and quantified by using agarose gel (1%) electrophoresis and spectrophotometric analysis (A260/A280 ratio). For cDNA synthesis, 2 µg total RNA was reverse-transcribed by using a Transcriptor First-Strand cDNA Synthesis kit (Roche Molecular Systems, Inc., Pleasanton, CA, USA), according to the manufacturers protocol. The qPCR was performed by using FastStart Universal SYBR-Green Master (ROX; Roche Molecular Systems, Inc.) on the Applied Biosystems 7500 Fast Real-Time PCR system (Applied Biosystems, Thermo Fisher Scientific, Inc.). The thermocycling parameters were 95°C for 3 min, followed by 40 cycles of 95°C for 15 sec and by 60°C for 30 sec. Each sample was run in triplicate and was normalized to U6 small nuclear RNA levels. The following primers were used for qPCR: miR-1284, 5′-GGTCTATACAGACCCTGGCTTTTC-3′ (forward) and 5′-CTCAACTGGTGTCGTGGA-3′ (reverse); and U6, 5′-TTATGGGTCCTAGCCTGAC-3′ (forward) and 5′-CACTATTGAGGGTATGC-3′ (reverse). All primers were synthesized by Shanghai GenePharma Co., Ltd. Melting curve analysis was performed to confirm the specificity of the PCR products. The replicates were subsequently averaged, and fold induction was determined by a ΔΔCq-based fold change calculation (17).

Cell viability assay

Cells in three groups (transfected with miR-1284, miR-1284 inhibitor and scramble control) were seeded into 96-well culture plates at 5×103 cells/well and cultured for 1–5 days. Each group had three replicates. Cell viability was then assayed by adding 20 µl 10 mg/ml 3-[4,5-dimethylthiazol-yl]-2,5-diphenyltetrazolium bromide (MTT; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), followed by incubation for 3 h at 37°C. Then 150 µl dimethyl sulfoxide was added to each well, and the optical density at 590 nm was recorded with a Multiskan EX (Thermo Fisher Scientific, Inc.). The results of three independent experiments are presented as the mean ± standard deviation.

Bromodeoxyuridine (BrdU) assay

Cells were seeded in 6-well plates at a density of 2×104 cells/well on sterilized coverslips. At 72 h after transfection, BrdU (Sigma-Aldrich; Merck KGaA) was added to the medium at a final concentration of 10 µM. After incubation at 37°C for 5 h in the dark, cells were fixed in cold 70% ethanol for 5 min. DNA was denaturized by adding 1.5 M HCl to the cells for 30 min at room temperature. Cells were washed with 150 µl PBS and 1% (v/v) bovine serum albumin (Sigma-Aldrich; Merck KGaA) at room temperature for 20 min for blocking. Immunofluorescence to visualize incorporated BrdU was performed using a mouse anti-BrdU antibody (cat no. 560808; 1:2,000; BD Biosciences, San Jose, CA, USA) according to the manufacturer's instructions, and incubated for 1 h at room temperature. Then, secondary red-fluorescence dye-conjugated antibody Alexa Fluor 594 (ab150116; 1:500; Abcam, Cambridge, UK) was added and incubated for 45 min at room temperature. VECTASHIELD mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA) was used to stain the nuclei. Images were visualized using a Leica inverted fully automated microscope (DMI6000B) with digital camera DFC 420 RGB (Leica Microsystems GmbH, Wetzlar, Germany).

Apoptosis assay

The cells (24 h after transfection) were seeded into culture dishes at a density of 2×105 cells per culture dish. Then cells were incubated with Annexin V-fluorescein isothiocyanate (BD Biosciences) and propidium iodide (PI; BD Biosciences) for 30 min in the dark at 25°C to analyze the apoptotic percentage of cells. Cells were analyzed with a FACSCalibur flow cytometer (BD Biosciences) and the results were analyzed by using FlowJo 10 software (Tree Star, Inc., Ashland, OR, USA).

Western blot analysis

Cells (48 h after transfection) were washed once with PBS and lysed in RIPA buffer (Pierce; Thermo Fisher Scientific, Inc.). Protein samples were quantified with the Pierce BCA Protein assay kit (Pierce; Thermo Fisher Scientific, Inc.) and were then boiled for 10 min in sodium dodecyl sulfate (SDS) sample buffer. Equal amounts of protein (20 µg/well) were separated by 10% SDS-polyacrylamide gel electrophoresis and transferred to nitrocellulose membrane. After blocking with 5% skim milk in 0.05% TBS-Tween-20 (v/v) for 1 h at room temperature, the membranes were incubated with the appropriate primary antibodies (at a dilution of 1:1,000) overnight at 4°C. Antibody specific to actin (C-2; cat no. 4967) was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA); c-Myc (cat no. ab32072), active caspase-3 (cat no. ab2302), p27 (cat no. ab54563), p21 (cat no. ab7960) and pro-caspase-3 (cat no. ab32150) were purchased from Abcam. Bax antibody (cat no. 2772) was purchased from Cell Signaling Technology, Inc. Then membranes were washed once with TBS-Tween-20 and incubated with anti-mouse and anti-rabbit horseradish peroxidase-conjugated secondary antibodies (cat nos. ab131368 and ab191866, respectively; 1:5,000) for 2 h at room temperature. Protein bands were visualized by using WEST-ZOL (plus) Western Blot Detection system (Intron Biotechnology, Inc., Seongnam, Korea).

Statistical analysis

Statistical analyses were performed using SPSS 19.0 statistical software (IBM SPSS, Armonk, NY, USA). All experiments were repeated three times. The results of multiple experiments were presented as the mean ± standard deviation. The data were initially tested for normal distribution using one-sample Kolmogorov-Smirnov test, and then analyzed by one-way analysis of variance followed by Dunnett's test for multiple comparisons. P<0.05 was considered to indicate a statistically significant difference.

Results

Role of miR-1284 in cell proliferation

Preliminary experiments to determine whether transfection with miR-1284 mimic and miR-1284 inhibitor successfully increased or decreased miR-1284 expression were performed, and the results indicated that transfection with miR-1284 mimic successfully increased the expression of miR-1284 and miR-1284 inhibitor transfection reduced levels, compared with control cells (Fig. 1). To determine the effect of miR-1284 on cell viability, A549 cells were transfected with miR-1284 or miR-1284 inhibitor and an MTT assay was performed. As demonstrated in Fig. 2, after transfection with miR-1284 mimic, the cell viability of A549 cells was decreased significantly compared with control (P<0.05). When cells were transfected with miR-1284 inhibitor, the cell viability was significantly increased compared with control cells (P<0.05).

Figure 1. A549 cells were transfected with miR-1284 mimic, miR-1284 inhibitor, or scramble control. Expression of miR-1284 in A549 cells was measured by quantitative polymerase chain reaction. ***P<0.001 vs. control group. miR, microRNA.

Figure 2. Cell viability assayed by MTT assay. Cell viability of A549 cells was decreased significantly upon miR-1284 overexpression while increased significantly upon miR-1284 inhibition. Values are presented as the mean ± standard deviation. *P<0.05 vs. control group at the same time point. miR, microRNA.

Role of miR-1284 in cell growth

BrdU staining was used to determine the role of miR-1284 in cell growth. Compared with the control group, a 20.7% decrease of BrdU-positive cells was detected in response to miR-1284 overexpression (P<0.05), whereas the miR-1284 inhibitor caused a 7.3% increase compared with the control group, however, this was not statistically significant (Fig. 3A and B).

Figure 3. (A) Immunofluorescence analysis of BrdU incorporation in A549 cells transfected with miR-1284 mimic or miR-1284 inhibitor. BrdU was stained with AlexaFluor594-conjugated antibodies (red). Cell nuclei were visualized by DAPI staining. (B) Quantitation of BrdU staining results (n=3). (C) Protein expression of p27 and p21 detected by western blot analysis. Values are presented as the mean ± standard deviation. *P<0.05 vs. control group. BrdU, bromodeoxyuridine; miR, microRNA.

To further understand the mechanisms of miR-1284 induced cell growth inhibition, we analyzed the effect of miR-1284 on cell cycle regulators in A549 cells. A strong increase in p27 was detected in response to the miR-1284 expression compared with the control. The other major cyclin-dependent kinase inhibitor 1 family member, p21, was unaffected by miR-1284 overexpression or inhibition (Fig. 3C).

Role of miR-1284 in apoptosis

To analyze the effect of miR-1284 on cell apoptosis, A549 cells were transfected with miR-1284 mimic or inhibitor and an apoptosis assay was performed. As presented in Fig. 4A and B, following transfection with miR-1284 mimic in A549 cells, the percentage of apoptotic cells was significantly increased from 8.0±1.0% (control group) to 27.6±3.2% (P<0.05). However, when cells were transfected with miR-1284 inhibitor, the percentage (3.09±0.6%) was decreased significantly compared with the control (P<0.05).

Figure 4. (A) Apoptotic cells were analyzed by flow cytometry to differentiate between apoptotic cells (annexin-V positive and PI-negative) and necrotic cells (annexin-V and PI-positive). Cells in Q3 were considered to be apoptotic cells and were compared between the groups. (B) Quantitation of flow cytometry apoptosis assay results. (C) Protein expression of Myc, Bax, pro-caspase-3 and active caspase-3 was determined by western blot analysis. Values are presented as the mean ± standard deviation. *P<0.05 vs. control group. miR, microRNA; PI, propidium iodide; FITC, fluorescein isothiocyanate; Bax, Bcl-2 associated X, apoptosis regulator; Q, quadrant.

miR-1284 may induce apoptosis by targeting Myc

To further understand the mechanisms of miR-1284 on apoptosis, the protein expressions of Myc, Bax, pro-caspase-3 and activated caspase-3 in the three groups were detected by western blot. The results in Fig. 4C demonstrated that, compared with control, the expression of Myc decreased markedly in the miR-1284 group, and increased markedly in the miR-1284 inhibitor group. For the other three proteins, their expressions in the miR-1284 group were higher than in the other two groups.

Discussion

The results of the present study demonstrated that miR-1284 has an important role in cell viability, growth and apoptosis of A549 cells. Following transfection with miR-1284, cell viability and growth decreased significantly, while apoptosis was significantly increased. By contrast, miR-1284 inhibition significantly increased the cell viability and growth, and decreased apoptosis in A549 cells. Furthermore, the expression of a cell cycle regulator, p27, was markedly increased and the expression of Myc was markedly decreased when miR-1284 was overexpressed in A549 cells. For the other three apoptosis-associated proteins, their expressions in miR-1284 mimic group were higher than in the control and miR-1284 inhibitor groups.

A previous study has reported that altered expression of miRNAs targeting cyclins, cyclin-dependent kinases (CDKs) or CDK inhibitors, such as p21 and p27, is involved in the deregulation of cell cycle progression during tumorigenesis (18). The results of the present study indicated that the expression of p27 was increased when miR-1284 was overexpressed, whereas p21 was unaffected by miR-1284 overexpression or inhibition. p27 belongs to the Cip/Kip family of CDK inhibitor proteins, which bind to cyclin D alone or its catalytic subunit CDK4 to control the cell cycle progression at G1 (19). In accordance with this, the present study demonstrated that cell growth was decreased significantly by miR-1284 overexpression, indicating that miR-1284 may induce cell growth arrest by upregulating p27 expression in A549 cells.

Apoptosis is a complex biological process that enables organisms to kill and remove unwanted cells during their development, normal homeostasis and disease (20,21). Evasion of apoptosis is a crucial step in the initiation and progression of cancer (22). The caspase family of cysteine proteases have key roles in the initiation and execution of apoptosis (23,24). Caspase-3 is a member of the caspase family that exists in the cell as a low-activity zymogen, pro-caspase-3. Pro-caspase-3 can be activated by proteolysis, and active caspase-3 is necessary for initiation of apoptosis (25). In the current study, miR-1284 overexpression significantly increased the apoptosis rate of A549 cells. Furthermore, the expression of pro-caspase-3 and active caspase-3 was also increased by miR-1284, which indicated that miR-1284 may be involved in accelerating the apoptosis of A549 cells.

In addition to pro-caspase-3 and active caspase-3, Bax expression was also increased by miR-1284 overexpression. Bax is a member of the Bcl-2 apoptosis regulator protein family, which has been studied intensively owing to their importance in the regulation of apoptosis and tumorigenesis (26). Bax is a pro-apoptotic protein and inactive in the cytosol of normal cells but becomes active in apoptotic cells by changing conformation in a multistep process (27). In accordance with this, the results of the present study also demonstrated that Bax was upregulated in apoptotic cells, further indicating that miR-1284 accelerates apoptosis.

Unlike the aforementioned proteins, the expression of Myc was decreased in the miR-1284 overexpression group and increased in the miR-1284 inhibitor group compared with control. Myc protein is a multifunctional, nuclear phosphoprotein that has a role in apoptosis. Aberrantly high or deregulated Myc activity is usually implicated in various types of cancer, and is often associated with aggressive and poorly differentiated tumors (28). Cell-autonomous apoptosis following Myc overexpression has been regarded as a major tumor-suppression mechanism (29). Romero et al (30) reported that amplifications of Myc are one of the most consistent gene alterations detected in lung cancer. In the current study, overexpression of Myc was observed in the miR-1284 inhibition group, which also had the lowest apoptosis rate, suggesting that Myc may be associated with apoptosis in these cells. Additionally, the results of the present study also indicated that miR-1284 may induce apoptosis by targeting Myc in A549 lung cancer cells.

In conclusion, the current study suggests that miR-1284 affects cell proliferation, growth and apoptosis of lung cancer cells, indicating that miR-1284 may have a key role in lung tumorigenesis. Thus, miR-1284 may be a potential therapeutic agent for the treatment of lung cancer.

References