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Article

Coronin 3 negatively regulates G6PC3 in HepG2 cells, as identified by label‑free mass‑spectrometry

  • Authors:
    • Yunzhen Gao
    • Ling Li
    • Xiaohua Xing
    • Minjie Lin
    • Yongyi Zeng
    • Xiaolong Liu
    • Jingfeng Liu
  • View Affiliations / Copyright

    Affiliations: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, Fujian 350005, P.R. China, Liver Disease Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, P.R. China
  • Pages: 3407-3414
    |
    Published online on: July 15, 2017
       https://doi.org/10.3892/mmr.2017.7002
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Abstract

Human coronin 3 is involved in many types of cancers, but the underlying molecular mechanisms require further elucidation. The present study demonstrated that coronin 3 is significantly upregulated in clinical primary hepatocellular carcinoma (HCC) samples by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and immunohistochemical staining. Subsequently, proteins that were regulated by coronin 3 in both coronin 3 overexpressing or knocked down HepG2 cells were analyzed by label free mass spectrometry; overall, 249 proteins were identified to be closely regulated by coronin 3, and those coronin 3 regulated proteins were enriched in cellular, physiological and metabolism processes. By further in‑depth pathway analysis, it was demonstrated that those proteins were involved into 94 different pathways. Finally, the expression levels of glucose‑6‑phosphatase catalytic subunit 3 (G6PC3) were confirmed to be negatively regulated by coronin 3, as determined by RT‑qPCR and western blotting. In conclusion, these results indicated that coronin3 is significantly dysregulated in HCC tumor tissues, and may exert its function via regulating G6PC3 expression. These results provide valuable information for further study of coronin 3‑mediated signaling pathways, and implicate coronin 3 as a potential therapeutic target for HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Gao Y, Li L, Xing X, Lin M, Zeng Y, Liu X and Liu J: Coronin 3 negatively regulates G6PC3 in HepG2 cells, as identified by label‑free mass‑spectrometry. Mol Med Rep 16: 3407-3414, 2017.
APA
Gao, Y., Li, L., Xing, X., Lin, M., Zeng, Y., Liu, X., & Liu, J. (2017). Coronin 3 negatively regulates G6PC3 in HepG2 cells, as identified by label‑free mass‑spectrometry. Molecular Medicine Reports, 16, 3407-3414. https://doi.org/10.3892/mmr.2017.7002
MLA
Gao, Y., Li, L., Xing, X., Lin, M., Zeng, Y., Liu, X., Liu, J."Coronin 3 negatively regulates G6PC3 in HepG2 cells, as identified by label‑free mass‑spectrometry". Molecular Medicine Reports 16.3 (2017): 3407-3414.
Chicago
Gao, Y., Li, L., Xing, X., Lin, M., Zeng, Y., Liu, X., Liu, J."Coronin 3 negatively regulates G6PC3 in HepG2 cells, as identified by label‑free mass‑spectrometry". Molecular Medicine Reports 16, no. 3 (2017): 3407-3414. https://doi.org/10.3892/mmr.2017.7002
Copy and paste a formatted citation
x
Spandidos Publications style
Gao Y, Li L, Xing X, Lin M, Zeng Y, Liu X and Liu J: Coronin 3 negatively regulates G6PC3 in HepG2 cells, as identified by label‑free mass‑spectrometry. Mol Med Rep 16: 3407-3414, 2017.
APA
Gao, Y., Li, L., Xing, X., Lin, M., Zeng, Y., Liu, X., & Liu, J. (2017). Coronin 3 negatively regulates G6PC3 in HepG2 cells, as identified by label‑free mass‑spectrometry. Molecular Medicine Reports, 16, 3407-3414. https://doi.org/10.3892/mmr.2017.7002
MLA
Gao, Y., Li, L., Xing, X., Lin, M., Zeng, Y., Liu, X., Liu, J."Coronin 3 negatively regulates G6PC3 in HepG2 cells, as identified by label‑free mass‑spectrometry". Molecular Medicine Reports 16.3 (2017): 3407-3414.
Chicago
Gao, Y., Li, L., Xing, X., Lin, M., Zeng, Y., Liu, X., Liu, J."Coronin 3 negatively regulates G6PC3 in HepG2 cells, as identified by label‑free mass‑spectrometry". Molecular Medicine Reports 16, no. 3 (2017): 3407-3414. https://doi.org/10.3892/mmr.2017.7002
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