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Article

Knockdown of JARID2 inhibits the viability and migration of placenta trophoblast cells in preeclampsia

  • Authors:
    • Yatao Jiang
    • Yuqing Chen
    • Yanping Chen
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Fujian Provincial Hospital, Fuzhou, Fujian 350001, P.R. China
  • Pages: 3594-3599
    |
    Published online on: July 15, 2017
       https://doi.org/10.3892/mmr.2017.7011
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Abstract

Protein Jumonji (JARID2) is a member of the Jumonji family of proteins and has been demonstrated to regulate cell proliferation and invasion. However, little is known about the role of JARID2 in the metastasis of placenta trophoblast cells. In the present study, the effect and the underlying molecular mechanism of JARID2 on trophoblast cell viability and invasion was investigated. The expression of JARID2 in placental tissues was analyzed by reverse transcription‑quantitative polymerase chain reaction and western blotting. HTR8/SVneo cells were transfected with si‑JARID2 or scramble for 24 h. Cell viability, migration and invasion in HTR8/SVneo cells were then evaluated. The expression levels of matrix metallopeptidase 2 (MMP2), MMP9, phosphorylated phosphatidylinositol 3‑kinase (p‑PI3K), PI3K, phosphorylated AKT serine/threonine kinase 1 (p‑Akt) and Akt in HTR8/SVneo cells were also detected using western blotting. The results of the present study demonstrated that JARID2 is underexpressed in human preeclamptic placentas. The knockdown of JARID2 significantly inhibited the viability, migration and invasion of HTR8/SVneo cells. In addition, the knockdown of JARID2 significantly decreased the levels of phosphorylated PI3K and Akt in HTR8/SVneo cells. The results of the present study demonstrated that JARID2 may serve a role in the progression of preeclampsia. The knockdown of JARID2 inhibited the viability and invasion of trophoblast cells in preeclampsia by suppressing the PI3K/Akt signaling pathway. Therefore, JARID2 may serve as a novel potential target for treating preeclampsia.
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1 

Backes CH, Markham K, Moorehead P, Cordero L, Nankervis CA and Giannone PJ: Maternal preeclampsia and neonatal outcomes. J Pregnancy. 2011:2143652011. View Article : Google Scholar : PubMed/NCBI

2 

Dieplinger H: Method for diagnosing preeclampsia. Patent WO2013000992 A1. Filed June 28, 2012; issued January 3. 2013.

3 

Bernardi F, Guolo F, Bortolin T, Petronilho F and Dal-Pizzol F: Oxidative stress and inflammatory markers in normal pregnancy and preeclampsia. J Obstet Gynaecol Res. 34:948–951. 2008.PubMed/NCBI

4 

Berzan E, Doyle R and Brown CM: Treatment of preeclampsia: Current approach and future perspectives. Curr Hypertens Rep. 16:4732014. View Article : Google Scholar : PubMed/NCBI

5 

Lyall F: Mechanisms regulating cytotrophoblast invasion in normal pregnancy and pre-eclampsia. Aust N Z J Obstet Gynaecol. 46:266–273. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Kooistra SM and Helin K: Molecular mechanisms and potential functions of histone demethylases. Nat Rev Mol Cell Biol. 13:297–311. 2012.PubMed/NCBI

7 

Herz HM and Shilatifard A: The JARID2-PRC2 duality. Genes Dev. 24:857–861. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Pasini D, Cloos PA, Walfridsson J, Olsson L, Bukowski JP, Johansen JV, Bak M, Tommerup N, Rappsilber J and Helin K: JARID2 regulates binding of the Polycomb repressive complex 2 to target genes in ES cells. Nature. 464:306–310. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Kinkel SA, Galeev R, Flensburg C, Keniry A, Breslin K, Gilan O, Lee S, Liu J, Chen K, Gearing LJ, et al: Jarid2 regulates hematopoietic stem cell function by acting with polycomb repressive complex 2. Blood. 125:1890–1900. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Mejetta S, Morey L, Pascual G, Kuebler B, Mysliwiec MR, Lee Y, Shiekhattar R, Di Croce L and Benitah SA: Jarid2 regulates mouse epidermal stem cell activation and differentiation. EMBO J. 30:3635–3646. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Tange S, Oktyabri D, Terashima M, Ishimura A and Suzuki T: JARID2 is involved in transforming growth factor-beta-induced epithelial-mesenchymal transition of lung and colon cancer cell lines. PLoS One. 9:e1156842014. View Article : Google Scholar : PubMed/NCBI

12 

Manceau G, Letouzé E, Guichard C, Didelot A, Cazes A, Corté H, Fabre E, Pallier K, Imbeaud S, Le Pimpec-Barthes F, et al: Recurrent inactivating mutations of ARID2 in non-small cell lung carcinoma. Int J Cancer. 132:2217–2221. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Lei X, Xu JF, Chang RM, Fang F, Zuo CH and Yang LY: JARID2 promotes invasion and metastasis of hepatocellular carcinoma by facilitating epithelial-mesenchymal transition through PTEN/AKT signaling. Oncotarget. 7:40266–40284. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-tie quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Su CL, Deng TR, Shang Z and Xiao Y: JARID2 inhibits leukemia cell proliferation by regulating CCND1 expression. Int J Hematol. 102:76–85. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Suga N, Sugimura M, Koshiishi T, Yorifuji T, Makino S and Takeda S: Heparin/heparan sulfate/CD44-v3 enhances cell migration in term placenta-derived immortalized human trophoblasts cells. Biol Reprod. 86:1341–8. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Zou Y, Jiang Z, Yu X, Sun M, Zhang Y, Zuo Q, Zhou J, Yang N, Han P, Ge Z, et al: Upregulation of long noncoding RNA SPRY4-IT1 modulates proliferaiton, migraiton, apoptosis, and netword formation in trophoblast cell HTR-8SV/neo. PLoS One. 8:e795982013. View Article : Google Scholar : PubMed/NCBI

18 

Kim YH, Kwon HJ and Kim DS: Matrix metalloproteinase 9 (MMP-9)-dependent processing of βig-h3 protein regulates cell migration, invasion, and adhesion. J Biol Chem. 287:38957–38969. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Chen Q, Jin M, Yang F, Zhu J, Xiao Q and Zhang L: Matrix Metalloproteinases: Inflammatory regulators of cell behaviors in vascular formation and remodeling. Mediators Inflamm. 2013:9283152013. View Article : Google Scholar : PubMed/NCBI

20 

Staun-Ram E, Goldman S, Gabarin D and Shalev E: Expression and importance of matrix metalloproteinase 2 and 9 (MMP-2 and −9) in human trophoblast invasion. Reprod Biol Endocrinol. 2:592004. View Article : Google Scholar : PubMed/NCBI

21 

Seval Y, Akkoyunlu G, Demir R and Asar M: Distribution patterns of metalloproteinase (MMP)-2 and-9 and their inhibitors (TIMP-1 and TIMP-2) in the human decidua during early pregnancy. Acta Histochem. 106:353–362. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Zheng Q, Dai K, Cui X, Yu M, Yang X, Yan B, Liu S and Yan Q: Leukemia inhibitory factor promote trophoblast invasion via urokinase-type plasminogen activator receptor in preeclampsia. Biomed Pharmacother. 80:102–108. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Jia RZ, Ding GC, Gu CM, Huang T, Rui C, Wang YX and Lu Q: CDX2 enhances HTR-8/SVneo trophoblast cell invasion by altering the expression of matrix metalloproteinases. Cell Physiol Biochem. 34:628–636. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Liu X, Mu H, Luo X, Xiao X, Ding Y, Yin N, Deng Q and Qi H: Expression of Gadd45α in human early placenta and its role in trophoblast invasion. Placenta. 35:370–377. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Cudmore MJ, Ahmad S, Sissaoui S, Ramma W, Ma B, Fujisawa T, Al-Ani B, Wang K, Cai M, Crispi F, et al: Loss of Akt activity increases circulating soluble endoglin release in preeclampsia: Identification of inter-dependency between Akt-1 and heme oxygenase-1. Eur Heart J. 33:1150–1158. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Bulj Z, Duchi S, Bevilacqua A, Gherardi A, Dozza B, Piccinini F, Mariani G Adalgisa, Lucarelli E, Giannini S, Donati D and Marmiroli S: Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells. Int J Oncol. 42:118–126. 2013.PubMed/NCBI

27 

Yoeli-Lerner M and Toker A: Akt/PKB signaling in cancer: A function in cell motility and invasion. Cell Cycle. 5:603–605. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Jiang Y, Chen Y and Chen Y: Knockdown of JARID2 inhibits the viability and migration of placenta trophoblast cells in preeclampsia. Mol Med Rep 16: 3594-3599, 2017.
APA
Jiang, Y., Chen, Y., & Chen, Y. (2017). Knockdown of JARID2 inhibits the viability and migration of placenta trophoblast cells in preeclampsia. Molecular Medicine Reports, 16, 3594-3599. https://doi.org/10.3892/mmr.2017.7011
MLA
Jiang, Y., Chen, Y., Chen, Y."Knockdown of JARID2 inhibits the viability and migration of placenta trophoblast cells in preeclampsia". Molecular Medicine Reports 16.3 (2017): 3594-3599.
Chicago
Jiang, Y., Chen, Y., Chen, Y."Knockdown of JARID2 inhibits the viability and migration of placenta trophoblast cells in preeclampsia". Molecular Medicine Reports 16, no. 3 (2017): 3594-3599. https://doi.org/10.3892/mmr.2017.7011
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang Y, Chen Y and Chen Y: Knockdown of JARID2 inhibits the viability and migration of placenta trophoblast cells in preeclampsia. Mol Med Rep 16: 3594-3599, 2017.
APA
Jiang, Y., Chen, Y., & Chen, Y. (2017). Knockdown of JARID2 inhibits the viability and migration of placenta trophoblast cells in preeclampsia. Molecular Medicine Reports, 16, 3594-3599. https://doi.org/10.3892/mmr.2017.7011
MLA
Jiang, Y., Chen, Y., Chen, Y."Knockdown of JARID2 inhibits the viability and migration of placenta trophoblast cells in preeclampsia". Molecular Medicine Reports 16.3 (2017): 3594-3599.
Chicago
Jiang, Y., Chen, Y., Chen, Y."Knockdown of JARID2 inhibits the viability and migration of placenta trophoblast cells in preeclampsia". Molecular Medicine Reports 16, no. 3 (2017): 3594-3599. https://doi.org/10.3892/mmr.2017.7011
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