Enhanced expression of natriuretic peptide receptor A and B in neutrophils of culprit lesions in patients with acute myocardial infarction

Nakagawa,Masashi; Naruko,Takahiko; Sugioka,Kenichi; Kitabayashi,Chizuko; Shirai,Nobuyuki; Takagi,Masahiko; Yoshiyama,Minoru; Ohsawa,Masahiko; Ueda,Makiko

doi:10.3892/mmr.2017.7034

Enhanced expression of natriuretic peptide receptor A and B in neutrophils of culprit lesions in patients with acute myocardial infarction

Authors:
- Masashi Nakagawa
- Takahiko Naruko
- Kenichi Sugioka
- Chizuko Kitabayashi
- Nobuyuki Shirai
- Masahiko Takagi
- Minoru Yoshiyama
- Masahiko Ohsawa
- Makiko Ueda
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Affiliations: Department of Cardiovascular Medicine, Osaka City University Graduate School of Medicine, Osaka 545‑8585, Japan, Department of Cardiology, Osaka City General Hospital, Osaka 534‑0021, Japan, Division of Nephrology and Hypertension, Osaka City General Hospital, Osaka 534‑0021, Japan, Department of Pathology, Osaka City University Graduate School of Medicine, Osaka 545‑8585, Japan, Department of Medical Technology, Morinomiya University of Medical Sciences, Osaka 559‑8611, Japan
Published online on: July 18, 2017 https://doi.org/10.3892/mmr.2017.7034
Pages: 3324-3330

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Abstract

Natriuretic peptides and their specific receptors have been suggested to have regulatory effects on smooth muscle cell (SMC) growth and inflammatory cell reactions. However, the roles of natriuretic peptide receptor A (NPR‑A) and B (NPR‑B) in unstable plaques remain to be studied in detail. Frozen sections from 82 coronary artery segments were used. These segments were obtained at autopsy from 13 patients with acute myocardial infarction (AMI; 7 ruptured and 6 eroded plaques) and from 30 patients with non‑cardiovascular diseases. Antibodies against SMCs, endothelial cells, macrophages, neutrophils, NPR‑A, NPR‑B and neutral endopeptidase (NEP) were used. Neutrophil infiltration was identified in all lesions with plaque rupture or erosion. Double immunostaining identified that the majority of NPR‑A‑ or NPR‑B‑positive cells were neutrophils in ruptured and eroded plaques. Using morphometric analysis, no significant difference was observed in the percentage of NPR‑A‑ and NPR‑B‑positive cells between ruptured and eroded plaques, while the number of NEP‑positive neutrophils in ruptured plaques was significantly higher compared with eroded plaques (P<0.0001). These results of the distinct presence of NPR‑A‑ and NPR‑B‑positive cells in unstable plaques underlying AMI suggested that natriuretic peptides serve a role in regulating plaque instability in humans.

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