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Ultrasound and magnetic resonance molecular imaging of atherosclerotic neovasculature with perfluorocarbon magnetic nanocapsules targeted against vascular endothelial growth factor receptor 2 in rats

  • Authors:
    • Hua Chen
    • Lianglong Chen
    • Rongxi Liang
    • Jin Wei
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Fujian Medical University Union Hospital, Fujian Institute of Coronary Heart Disease, Fuzhou, Fujian 350001, P.R. China, Department of Ultrasonography, Fujian Medical University Union Hospital, Fuzhou, Fujian 350001, P.R. China, Department of Imaging, Fujian Medical University Union Hospital, Fuzhou, Fujian 350001, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5986-5996
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    Published online on: August 22, 2017
       https://doi.org/10.3892/mmr.2017.7314
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Abstract

The aim of the present study was to investigate the feasibility of using ultrasonography (US) and magnetic resonance (MR) for bimodal molecular imaging of atherosclerotic neovasculature with liquid perfluorocarbon magnetic nanocapsules (NCs) targeted to vascular endothelial growth factor receptor 2 (VEGFR‑2). By incorporating perfluorooctyl bromide (PFOB) and superparamagnetic iron oxide (SPIO) into polylactic acid, a SPIO‑embedded PFOB NC was constructed; subsequently, a VEGFR‑2‑targeted NC (VTNC) containing dual detectable probes was created by covalently linking a VEGFR‑2 antibody onto the surface of the SPIO‑embedded PFOB NC. Target specificity was verified in vitro by incubating VTNC with VEGFR‑2+ or VEGFR‑2‑ endothelial cells. Rats with vulnerable plaques were assigned to receive either an injection of VTNC (Targeted group; n=8) or an injection of NC (Nontargeted group; n=8); control rats also received an injection of VTNC (Control group; n=8). US and MR imaging of the abdominal aorta were performed to detect VTNC by measuring of the ultrasonic grayscale intensity (GSI) and MR contrast‑to‑noise ratio (CNR) prior to and at successive time points following VTNC and NC injections. The percent positive area (PPA) of CD31+ (PPACD31+) or VEGFR‑2+ (PPAVEGFR‑2+) expression was quantified by immunohistochemical staining. CD31 was used to verify the existence of endothelial cells as it is widely expressed on the surface of endothelial cells whether activated or not. The results demonstrated that VTNC was able to highly and selectively detect VEGFR‑2+ endothelial cells, and GSI, CNR, PPACD31+ and PPAVEGFR‑2+ were significantly increased in the targeted group compared with the nontargeted and control groups. In the control group, no atherosclerotic plaques or angiogenesis was identified, thus no expression of PPACD31+ and PPAVEGFR‑2 (data not shown). There were strong correlations among GSI, CNR, PPACD31+ and PPAVEGFR‑2+. In conclusion, two‑probe VTNC is feasible for bimodal US and MR molecular imaging of atherosclerotic neovasculature, which may offer complementary information for the more reliable prediction of plaque vulnerability.
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1 

Hansson GK, Libby P and Tabas I: Inflammation and plaque vulnerability. J Intern Med. 278:483–493. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Falk E, Nakano M, Bentzon JF, Finn AV and Virmani R: Update on acute coronary syndromes: The pathologists' view. Eur Heart J. 34:719–728. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Haasdijk RA, Den Dekker WK, Cheng C, Tempel D, Szulcek R, Bos FL, Hermkens DM, Chrifi I, Brandt MM, Van Dijk C, et al: THSD1 preserves vascular integrity and protects against intraplaque haemorrhaging in ApoE−/− mice. Cardiovasc Res. 110:129–139. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Kolodgie FD, Gold HK, Burke AP, Fowler DR, Kruth HS, Weber DK, Farb A, Guerrero LJ, Hayase M, Kutys R, et al: Intraplaque hemorrhage and progression of coronary atheroma. N Engl J Med. 349:2316–2325. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Moreno PR, Purushothaman KR, Zias E, Sanz J and Fuster V: Neovascularization in human atherosclerosis. Curr Mol Med. 6:457–477. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Taruya A, Tanaka A, Nishiguchi T, Matsuo Y, Ozaki Y, Kashiwagi M, Shiono Y, Orii M, Yamano T, Ino Y, et al: Vasa Vasorum Restructuring in Human Atherosclerotic Plaque Vulnerability: A clinical optical coherence tomography study. J Am Coll Cardiol. 65:2469–2477. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Chang SH, Feng D, Nagy JA, Sciuto TE, Dvorak AM and Dvorak HF: Vascular permeability and pathological angiogenesis in caveolin-1-null mice. Am J Pathol. 175:1768–1776. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Michel JB, Martin-Ventura JL, Nicoletti A and Ho-Tin-Noe B: Pathology of human plaque vulnerability: Mechanisms and consequences of intraplaque haemorrhages. Atherosclerosis. 234:311–319. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Brogi E, Wu T, Namiki A and Isner JM: Indirect angiogenic cytokines upregulate VEGF and bFGF gene expression in vascular smooth muscle cells, whereas hypoxia upregulates VEGF expression only. Circulation. 90:649–652. 1994. View Article : Google Scholar : PubMed/NCBI

10 

Ahmad S, Hewett PW, Wang P, Al-Ani B, Cudmore M, Fujisawa T, Haigh JJ, le Noble F, Wang L, Mukhopadhyay D and Ahmed A: Direct evidence for endothelial vascular endothelial growth factor receptor-1 function in nitric oxide-mediated angiogenesis. Circ Res. 99:715–722. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Belgore F, Blann A, Neil D, Ahmed AS and Lip GY: Localisation of members of the vascular endothelial growth factor (VEGF) family and their receptors in human atherosclerotic arteries. J Clin Pathol. 57:266–272. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Perrot-Applanat M and Di Benedetto M: Autocrine functions of VEGF in breast tumor cells: Adhesion, survival, migration and invasion. Cell Adh Migr. 6:547–553. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Choi HS, Kim MK, Lee K, Lee KM, Choi YK, Shin YC, Cho SG and Ko SG: SH003 represses tumor angiogenesis by blocking VEGF binding to VEGFR2. Oncotarget. 7:32967–32979. 2016.

14 

Mancini M, Greco A, Salvatore G, Liuzzi R, Di Maro G, Vergara E, Chiappetta G, Pasquinelli R, Brunetti A and Salvatore M: Imaging of thyroid tumor angiogenesis with microbubbles targeted to vascular endothelial growth factor receptor type 2 in mice. BMC Med Imaging. 13:312013. View Article : Google Scholar : PubMed/NCBI

15 

Tekabe Y, Kollaros M, Zerihoun A, Zhang G, Backer MV, Backer JM and Johnson LL: Imaging VEGF receptor expression to identify accelerated atherosclerosis. EJNMMI Res. 4:412014. View Article : Google Scholar : PubMed/NCBI

16 

Wang K, Pan D, Schmieder AH, Senpan A, Caruthers SD, Cui G, Allen JS, Zhang H, Shen B and Lanza GM: Atherosclerotic neovasculature MR imaging with mixed manganese-gadolinium nanocolloids in hyperlipidemic rabbits. Nanomedicine. 11:569–578. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Schinkel AF, van den Oord SC, Van Der Steen AF, van Laar JA and Sijbrands EJ: Utility of contrast-enhanced ultrasound for the assessment of the carotid artery wall in patients with Takayasu or giant cell arteritis. Eur Heart J Cardiovasc Imaging. 15:541–546. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Romero JM, Pizzolato R, Atkinson W, Meader A, Jaimes C, Lamuraglia G, Jaff MR, Buonanno F, Almandoz J Delgado and Gonzalez RG: Vasa vasorum enhancement on computerized tomographic angiography correlates with symptomatic patients with 50 to 70% carotid artery stenosis. Stroke. 44:3344–3349. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Hrkach J, Von Hoff D, Ali M Mukkaram, Andrianova E, Auer J, Campbell T, De Witt D, Figa M, Figueiredo M, Horhota A, et al: Preclinical development and clinical translation of a PSMA-targeted docetaxel nanoparticle with a differentiated pharmacological profile. Sci Transl Med. 4:128ra392012. View Article : Google Scholar : PubMed/NCBI

20 

Pisani E, Tsapis N, Galaz B, Santin M, Berti R, Taulier N, Kurtisovski E, Lucidarme O, Ourevitch M, Doan BT, et al: Perfluorooctyl Bromide Polymeric Capsules as Dual Contrast Agents for Ultrasonography and Magnetic Resonance Imaging. Adv Funct Mater. 18:2963–2971. 2008. View Article : Google Scholar

21 

Ghosh D, Lee Y, Thomas S, Kohli AG, Yun DS, Belcher AM and Kelly KA: M13-templated magnetic nanoparticles for targeted in vivo imaging of prostate cancer. Nat Nanotechnol. 7:677–682. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Li S, Ma Y, Yue X, Cao Z and Dai Z: One-pot construction of doxorubicin conjugated magnetic silica nanoparticles. New J Chem. 33:2414–2418. 2009. View Article : Google Scholar

23 

Liu J, Li J, Rosol TJ, Pan X and Voorhees JL: Biodegradable nanoparticles for targeted ultrasound imaging of breast cancer cells in vitro. Phys Med Biol. 52:4739–4747. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Hermanson GT: Bioconjugate Techniques. I. Academic Press; London: pp. 169–172. 1996, View Article : Google Scholar

25 

Tian C, Zhang R, Ye X, Zhang C, Jin X, Yamori Y, Hao L, Sun X and Ying C: Resveratrol ameliorates high-glucose-induced hyperpermeability mediated by caveolae via VEGF/KDR pathway. Genes Nutr. 8:231–239. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Fonseca FA, Paiva TB, Silva EG, Ihara SS, Kasinski N, Martinez TL and Filho EE: Dietary magnesium improves endothelial dependent relaxation of balloon injured arteries in rats. Atherosclerosis. 139:237–242. 1998. View Article : Google Scholar : PubMed/NCBI

27 

Yan F, Yang W, Li X, Liu H, Nan X, Xie L, Zhou D, Xie G, Wu J, Qiu B, et al: Magnetic resonance imaging of atherosclerosis using CD81-targeted microparticles of iron oxide in mice. Biomed Res Int. 2015:7586162015. View Article : Google Scholar : PubMed/NCBI

28 

Guo S, Shen S, Wang J, Wang H, Li M, Liu Y, Hou F, Liao Y and Bin J: Detection of high-risk atherosclerotic plaques with ultrasound molecular imaging of glycoprotein IIb/IIIa receptor on activated platelets. Theranostics. 5:418–430. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Reimer P and Balzer T: Ferucarbotran (Resovist): A new clinically approved RES-specific contrast agent for contrast-enhanced MRI of the liver: Properties, clinical development, and applications. Eur Radiol. 13:1266–1276. 2003.PubMed/NCBI

30 

Wiesner P, Tafelmeier M, Chittka D, Choi SH, Zhang L, Byun YS, Almazan F, Yang X, Iqbal N, Chowdhury P, et al: MCP-1 binds to oxidized LDL and is carried by lipoprotein(a) in human plasma. J Lipid Res. 54:1877–1883. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Hsu WY, Chao YW, Tsai YL, Lien CC, Chang CF, Deng MC, Ho LT, Kwok CF and Juan CC: Resistin induces monocyte-endothelial cell adhesion by increasing ICAM-1 and VCAM-1 expression in endothelial cells via p38MAPK-dependent pathway. J Cell Physiol. 226:2181–2188. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Fotis L, Agrogiannis G, Vlachos IS, Pantopoulou A, Margoni A, Kostaki M, Verikokos C, Tzivras D, Mikhailidis DP and Perrea D: Intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 at the early stages of atherosclerosis in a rat model. In Vivo. 26:243–250. 2012.PubMed/NCBI

33 

Deyama J, Nakamura T, Takishima I, Fujioka D, Kawabata K, Obata JE, Watanabe K, Watanabe Y, Saito Y, Mishina H and Kugiyama K: Contrast-enhanced ultrasound imaging of carotid plaque neovascularization is useful for identifying high-risk patients with coronary artery disease. Circ J. 77:1499–1507. 2013. View Article : Google Scholar : PubMed/NCBI

34 

Li X, Bauer W, Israel I, Kreissl MC, Weirather J, Richter D, Bauer E, Herold V, Jakob P, Buck A, et al: Targeting P-selectin by gallium-68-labeled fucoidan positron emission tomography for noninvasive characterization of vulnerable plaques: Correlation with in vivo 17.6T MRI. Arterioscler Thromb Vasc Biol. 34:1661–1667. 2014. View Article : Google Scholar : PubMed/NCBI

35 

Daeichin V, Kooiman K, Skachkov I, Bosch JG, Theelen TL, Steiger K, Needles A, Janssen BJ, Daemen MJ, Van Der Steen AF, et al: Quantification of Endothelial αvβ3 expression with high-frequency ultrasound and targeted Microbubbles: In vitro and In vivo Studies. Ultrasound Med Biol. 42:2283–2293. 2016. View Article : Google Scholar : PubMed/NCBI

36 

Qi CM, Du L, Wu WH, Li DY, Hao J, Gong L, Deng L, Zhang T, Zhang C and Zhang Y: Detection of Vulnerable Atherosclerotic Plaques in Experimental Atherosclerosis with the USPIO-Enhanced MRI. Cell Biochem Biophys. 73:331–337. 2015. View Article : Google Scholar : PubMed/NCBI

37 

Wang Y, Chen J, Yang B, Qiao H, Gao L, Su T, Ma S, Zhang X, Li X, Liu G, et al: In vivo MR and Fluorescence Dual-modality Imaging of Atherosclerosis Characteristics in Mice Using Profilin-1 Targeted Magnetic Nanoparticles. Theranostics. 6:272–286. 2016. View Article : Google Scholar : PubMed/NCBI

38 

van Tilborg GA, Vucic E, Strijkers GJ, Cormode DP, Mani V, Skajaa T, Reutelingsperger CP, Fayad ZA, Mulder WJ and Nicolay K: Annexin A5-functionalized bimodal nanoparticles for MRI and fluorescence imaging of atherosclerotic plaques. Bioconjug Chem. 21:1794–1803. 2010. View Article : Google Scholar : PubMed/NCBI

39 

Gerber HP, McMurtrey A, Kowalski J, Yan M, Keyt BA, Dixit V and Ferrara N: Vascular endothelial growth factor regulates endothelial cell survival through the phosphatidylinositol 3′-kinase/Akt signal transduction pathway. Requirement for Flk-1/KDR activation. J Biol Chem. 273:30336–30343. 1998. View Article : Google Scholar : PubMed/NCBI

40 

Arnott C, Punnia-Moorthy G, Tan J, Sadeghipour S, Bursill C and Patel S: The Vascular Endothelial Growth Factor Inhibitors Ranibizumab and Aflibercept Markedly Increase Expression of Atherosclerosis-Associated Inflammatory Mediators on Vascular Endothelial Cells. PLoS One. 11:e01506882016. View Article : Google Scholar : PubMed/NCBI

41 

Kiessling F, Gaetjens J and Palmowski M: Application of molecular ultrasound for imaging integrin expression. Theranostics. 1:127–134. 2011. View Article : Google Scholar : PubMed/NCBI

42 

Eckert MA, Vu PQ, Zhang K, Kang D, Ali MM, Xu C and Zhao W: Novel molecular and nanosensors for in vivo sensing. Theranostics. 3:583–594. 2013. View Article : Google Scholar : PubMed/NCBI

43 

Díaz-López R, Tsapis N and Fattal E: Liquid perfluorocarbons as contrast agents for ultrasonography and (19)F-MRI. Pharm Res. 27:1–16. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen H, Chen L, Liang R and Wei J: Ultrasound and magnetic resonance molecular imaging of atherosclerotic neovasculature with perfluorocarbon magnetic nanocapsules targeted against vascular endothelial growth factor receptor 2 in rats. Mol Med Rep 16: 5986-5996, 2017.
APA
Chen, H., Chen, L., Liang, R., & Wei, J. (2017). Ultrasound and magnetic resonance molecular imaging of atherosclerotic neovasculature with perfluorocarbon magnetic nanocapsules targeted against vascular endothelial growth factor receptor 2 in rats. Molecular Medicine Reports, 16, 5986-5996. https://doi.org/10.3892/mmr.2017.7314
MLA
Chen, H., Chen, L., Liang, R., Wei, J."Ultrasound and magnetic resonance molecular imaging of atherosclerotic neovasculature with perfluorocarbon magnetic nanocapsules targeted against vascular endothelial growth factor receptor 2 in rats". Molecular Medicine Reports 16.5 (2017): 5986-5996.
Chicago
Chen, H., Chen, L., Liang, R., Wei, J."Ultrasound and magnetic resonance molecular imaging of atherosclerotic neovasculature with perfluorocarbon magnetic nanocapsules targeted against vascular endothelial growth factor receptor 2 in rats". Molecular Medicine Reports 16, no. 5 (2017): 5986-5996. https://doi.org/10.3892/mmr.2017.7314
Copy and paste a formatted citation
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Spandidos Publications style
Chen H, Chen L, Liang R and Wei J: Ultrasound and magnetic resonance molecular imaging of atherosclerotic neovasculature with perfluorocarbon magnetic nanocapsules targeted against vascular endothelial growth factor receptor 2 in rats. Mol Med Rep 16: 5986-5996, 2017.
APA
Chen, H., Chen, L., Liang, R., & Wei, J. (2017). Ultrasound and magnetic resonance molecular imaging of atherosclerotic neovasculature with perfluorocarbon magnetic nanocapsules targeted against vascular endothelial growth factor receptor 2 in rats. Molecular Medicine Reports, 16, 5986-5996. https://doi.org/10.3892/mmr.2017.7314
MLA
Chen, H., Chen, L., Liang, R., Wei, J."Ultrasound and magnetic resonance molecular imaging of atherosclerotic neovasculature with perfluorocarbon magnetic nanocapsules targeted against vascular endothelial growth factor receptor 2 in rats". Molecular Medicine Reports 16.5 (2017): 5986-5996.
Chicago
Chen, H., Chen, L., Liang, R., Wei, J."Ultrasound and magnetic resonance molecular imaging of atherosclerotic neovasculature with perfluorocarbon magnetic nanocapsules targeted against vascular endothelial growth factor receptor 2 in rats". Molecular Medicine Reports 16, no. 5 (2017): 5986-5996. https://doi.org/10.3892/mmr.2017.7314
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