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Article Open Access

AKT2 is involved in the IL‑17A‑mediated promotion of differentiation and calcification of murine preosteoblastic MC3T3‑E1 cells

  • Authors:
    • Jing‑Yi Tan
    • Li‑Hong Lei
    • Xiao‑Tao Chen
    • Pei‑Hui Ding
    • Yan‑Min Wu
    • Li‑Li Chen
  • View Affiliations / Copyright

    Affiliations: Department of Oral Medicine, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China, Department of Oral Medicine, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 830001, P.R. China
    Copyright: © Tan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5833-5840
    |
    Published online on: August 22, 2017
       https://doi.org/10.3892/mmr.2017.7315
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Abstract

Interleukin (IL)‑17A exhibits pleiotropic biological activities and serves a role in the progression of periodontitis. However, data describing the association between IL‑17 and osteogenesis are not conclusive. It was previously demonstrated that RAC‑β serine/threonine protein kinase (AKT2)‑specific knockdown in MC3T3‑E1 cells weakened osteogenic effects. The role of AKT2 in the regulation of IL‑17A for osteoblast differentiation and calcification remains unclear. The MTT method was adopted in the present study to assess cell proliferation; cell cycle distribution was analyzed by flow cytometry. Following osteogenic induction treatment, the involvement of phosphatidylinositol 3‑kinase (PI3K) and phosphorylated‑PI3K was evaluated by western blotting. The effects of IL‑17A on osteogenesis‑associated markers, including Runt‑related transcription factor 2 (Runx‑2), alkaline phosphatase (ALP) and osteocalcin (OCN) were evaluated by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis. An ALP activity assay and Alizarin Red S staining were used to assess the differentiation and calcification functions. AKT2 knockdown inhibited MC3T3‑E1 cell proliferation, inducing significantly increased G0/G1 cell counts, and reduced S and G2/M cell numbers. IL‑17A exerted no significant effects. The protein levels of p‑PI3K, gene expression levels of IL‑17A, Runx‑2, ALP and OCN, and relative ALP activity and calcification areas were increased in the induction group, and these effects were markedly promoted by treatment with IL‑17A. AKT2 knockdown in MC3T3‑E1 cells resulted in reduced IL‑17A‑induced differentiation and calcification, although it was not completely inhibited. The results of the present study suggested that AKT2 signaling was required for MC3T3‑E1 cell proliferation. IL‑17A promoted osteoblast differentiation and calcification in a partly AKT2‑dependent manner in MC3T3‑E1 cells in vitro, possibly reflecting compensation by other signaling pathways. The results of the present study may offer novel perspectives to guide the clinical strategy for the prevention and treatment of periodontitis.
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Copy and paste a formatted citation
Spandidos Publications style
Tan JY, Lei LH, Chen XT, Ding PH, Wu YM and Chen LL: AKT2 is involved in the IL‑17A‑mediated promotion of differentiation and calcification of murine preosteoblastic MC3T3‑E1 cells. Mol Med Rep 16: 5833-5840, 2017.
APA
Tan, J., Lei, L., Chen, X., Ding, P., Wu, Y., & Chen, L. (2017). AKT2 is involved in the IL‑17A‑mediated promotion of differentiation and calcification of murine preosteoblastic MC3T3‑E1 cells. Molecular Medicine Reports, 16, 5833-5840. https://doi.org/10.3892/mmr.2017.7315
MLA
Tan, J., Lei, L., Chen, X., Ding, P., Wu, Y., Chen, L."AKT2 is involved in the IL‑17A‑mediated promotion of differentiation and calcification of murine preosteoblastic MC3T3‑E1 cells". Molecular Medicine Reports 16.5 (2017): 5833-5840.
Chicago
Tan, J., Lei, L., Chen, X., Ding, P., Wu, Y., Chen, L."AKT2 is involved in the IL‑17A‑mediated promotion of differentiation and calcification of murine preosteoblastic MC3T3‑E1 cells". Molecular Medicine Reports 16, no. 5 (2017): 5833-5840. https://doi.org/10.3892/mmr.2017.7315
Copy and paste a formatted citation
x
Spandidos Publications style
Tan JY, Lei LH, Chen XT, Ding PH, Wu YM and Chen LL: AKT2 is involved in the IL‑17A‑mediated promotion of differentiation and calcification of murine preosteoblastic MC3T3‑E1 cells. Mol Med Rep 16: 5833-5840, 2017.
APA
Tan, J., Lei, L., Chen, X., Ding, P., Wu, Y., & Chen, L. (2017). AKT2 is involved in the IL‑17A‑mediated promotion of differentiation and calcification of murine preosteoblastic MC3T3‑E1 cells. Molecular Medicine Reports, 16, 5833-5840. https://doi.org/10.3892/mmr.2017.7315
MLA
Tan, J., Lei, L., Chen, X., Ding, P., Wu, Y., Chen, L."AKT2 is involved in the IL‑17A‑mediated promotion of differentiation and calcification of murine preosteoblastic MC3T3‑E1 cells". Molecular Medicine Reports 16.5 (2017): 5833-5840.
Chicago
Tan, J., Lei, L., Chen, X., Ding, P., Wu, Y., Chen, L."AKT2 is involved in the IL‑17A‑mediated promotion of differentiation and calcification of murine preosteoblastic MC3T3‑E1 cells". Molecular Medicine Reports 16, no. 5 (2017): 5833-5840. https://doi.org/10.3892/mmr.2017.7315
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