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Staphylococcus aureus Protein A induces osteoclastogenesis via the NF‑κB signaling pathway

  • Authors:
    • Li‑Rong Ren
    • Hai Wang
    • Xiao‑Qing He
    • Mu‑Guo Song
    • Xue‑Qiu Chen
    • Yong‑Qing Xu
  • View Affiliations / Copyright

    Affiliations: Department of Traumatology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, P.R. China, Department of Orthopedic Surgery, Kunming General Hospital of Chengdu Military Command, Kunming, Yunnan 650032, P.R. China
    Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6020-6028
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    Published online on: August 22, 2017
       https://doi.org/10.3892/mmr.2017.7316
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Abstract

Staphylococcus aureus (S. aureus) is the most common organism causing osteomyelitis, and Staphylococcus aureus protein A (SpA) is an important virulence factor anchored in its cell wall. However, the precise mechanisms underlying the bone loss caused by SpA have not been well understood. The present study aimed to investigate the effect of SpA on osteoclast differentiation, and the probable mechanism was investigated. Raw264.7 cells were treated with SpA in the absence or presence of receptor‑activated (NF)‑κB ligand for 5 days, and morphological and biochemical assays were used to assess osteoclastogenesis and explore the underlying mechanisms. Data demonstrated that SpA induced osteoclast differentiation and promoted bone resorption in a dose‑dependent manner in the absence or presence of RANKL. In addition, the expression of osteoclast‑specific genes, such as the tartrate resistant acid phosphatase, matrix metalloproteinase‑9, cathepsin K, calcitonin receptors and d2 isoform of the vacuolar ATPase Vo domain, were enhanced by SpA. Furthermore, the SpA‑induced osteoclast differentiation was associated with the degradation of inhibitor of κB‑α, phosphorylation of NF‑κB p65 and increased expression of nuclear factor of activated T‑cells. However, by treatment with JSH‑23, an NF‑κB inhibitor, the formation of osteoclast‑like cells and resorption pits was significantly reduced, and the expression of osteoclast‑specific genes was also inhibited. Collectively, in the present study SpA induced osteoclast differentiation, promoted bone resorption, and the NF‑κB signaling pathway was involved in this process.
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Copy and paste a formatted citation
Spandidos Publications style
Ren LR, Wang H, He XQ, Song MG, Chen XQ and Xu YQ: Staphylococcus aureus Protein A induces osteoclastogenesis via the NF‑κB signaling pathway. Mol Med Rep 16: 6020-6028, 2017.
APA
Ren, L., Wang, H., He, X., Song, M., Chen, X., & Xu, Y. (2017). Staphylococcus aureus Protein A induces osteoclastogenesis via the NF‑κB signaling pathway. Molecular Medicine Reports, 16, 6020-6028. https://doi.org/10.3892/mmr.2017.7316
MLA
Ren, L., Wang, H., He, X., Song, M., Chen, X., Xu, Y."Staphylococcus aureus Protein A induces osteoclastogenesis via the NF‑κB signaling pathway". Molecular Medicine Reports 16.5 (2017): 6020-6028.
Chicago
Ren, L., Wang, H., He, X., Song, M., Chen, X., Xu, Y."Staphylococcus aureus Protein A induces osteoclastogenesis via the NF‑κB signaling pathway". Molecular Medicine Reports 16, no. 5 (2017): 6020-6028. https://doi.org/10.3892/mmr.2017.7316
Copy and paste a formatted citation
x
Spandidos Publications style
Ren LR, Wang H, He XQ, Song MG, Chen XQ and Xu YQ: Staphylococcus aureus Protein A induces osteoclastogenesis via the NF‑κB signaling pathway. Mol Med Rep 16: 6020-6028, 2017.
APA
Ren, L., Wang, H., He, X., Song, M., Chen, X., & Xu, Y. (2017). Staphylococcus aureus Protein A induces osteoclastogenesis via the NF‑κB signaling pathway. Molecular Medicine Reports, 16, 6020-6028. https://doi.org/10.3892/mmr.2017.7316
MLA
Ren, L., Wang, H., He, X., Song, M., Chen, X., Xu, Y."Staphylococcus aureus Protein A induces osteoclastogenesis via the NF‑κB signaling pathway". Molecular Medicine Reports 16.5 (2017): 6020-6028.
Chicago
Ren, L., Wang, H., He, X., Song, M., Chen, X., Xu, Y."Staphylococcus aureus Protein A induces osteoclastogenesis via the NF‑κB signaling pathway". Molecular Medicine Reports 16, no. 5 (2017): 6020-6028. https://doi.org/10.3892/mmr.2017.7316
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