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Subcutaneous injection of dendritic cells aggravates atherosclerosis in ApoE‑knockout mice by activation of TLR4

  • Authors:
    • Zhishuai Ye
    • Mingyu Jin
    • Shujing Wang
    • Jianing Zhang
    • Xiantao Song
    • Rongchong Huang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China, College of Life Sciences and Pharmacy, Dalian University of Technology, Dalian, Liaoning 116027, P.R. China, Department of Cardiology, Capital Medical University Affiliated Beijing Anzhen Hospital, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing 100029, P.R. China
    Copyright: © Ye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6041-6049
    |
    Published online on: August 23, 2017
       https://doi.org/10.3892/mmr.2017.7339
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Abstract

Dendritic cells (DCs) are specialized antigen‑presenting cells which are important in immune diseases, in particular atherosclerosis, a chronic inflammatory disease, however their role in atherosclerosis‑associated immunity is unclear. To evaluate the role of DCs in atherosclerosis, exogenous bone marrow‑derived DCs were transferred into ApoE‑/‑ mice in the present study. The extent of disease was measured in the aorta and was compared with mice treated with phosphate‑buffered saline (PBS) or left untreated and fed a western diet. Mice receiving exogenous DCs demonstrated significantly larger atherosclerotic lesions compared with the mice treated with PBS, with increasing numbers of mature DCs in circulation and enhanced DC infiltration into plaque lesions, in addition to activation of circulating inflammatory components and atherosclerotic lesions. Furthermore, it was demonstrated that exogenous DCs upregulated the expression of Toll‑like receptor 4 (TLR4) on DCs, which may be an important mechanism to activate DCs and aggravate atherosclerosis. Therefore the present study concluded that exogenous DCs may induce maturation of endogenous DCs via upregulation of TLR4, further increasing the inflammatory response and accelerating atherosclerosis.
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Copy and paste a formatted citation
Spandidos Publications style
Ye Z, Jin M, Wang S, Zhang J, Song X and Huang R: Subcutaneous injection of dendritic cells aggravates atherosclerosis in ApoE‑knockout mice by activation of TLR4. Mol Med Rep 16: 6041-6049, 2017.
APA
Ye, Z., Jin, M., Wang, S., Zhang, J., Song, X., & Huang, R. (2017). Subcutaneous injection of dendritic cells aggravates atherosclerosis in ApoE‑knockout mice by activation of TLR4. Molecular Medicine Reports, 16, 6041-6049. https://doi.org/10.3892/mmr.2017.7339
MLA
Ye, Z., Jin, M., Wang, S., Zhang, J., Song, X., Huang, R."Subcutaneous injection of dendritic cells aggravates atherosclerosis in ApoE‑knockout mice by activation of TLR4". Molecular Medicine Reports 16.5 (2017): 6041-6049.
Chicago
Ye, Z., Jin, M., Wang, S., Zhang, J., Song, X., Huang, R."Subcutaneous injection of dendritic cells aggravates atherosclerosis in ApoE‑knockout mice by activation of TLR4". Molecular Medicine Reports 16, no. 5 (2017): 6041-6049. https://doi.org/10.3892/mmr.2017.7339
Copy and paste a formatted citation
x
Spandidos Publications style
Ye Z, Jin M, Wang S, Zhang J, Song X and Huang R: Subcutaneous injection of dendritic cells aggravates atherosclerosis in ApoE‑knockout mice by activation of TLR4. Mol Med Rep 16: 6041-6049, 2017.
APA
Ye, Z., Jin, M., Wang, S., Zhang, J., Song, X., & Huang, R. (2017). Subcutaneous injection of dendritic cells aggravates atherosclerosis in ApoE‑knockout mice by activation of TLR4. Molecular Medicine Reports, 16, 6041-6049. https://doi.org/10.3892/mmr.2017.7339
MLA
Ye, Z., Jin, M., Wang, S., Zhang, J., Song, X., Huang, R."Subcutaneous injection of dendritic cells aggravates atherosclerosis in ApoE‑knockout mice by activation of TLR4". Molecular Medicine Reports 16.5 (2017): 6041-6049.
Chicago
Ye, Z., Jin, M., Wang, S., Zhang, J., Song, X., Huang, R."Subcutaneous injection of dendritic cells aggravates atherosclerosis in ApoE‑knockout mice by activation of TLR4". Molecular Medicine Reports 16, no. 5 (2017): 6041-6049. https://doi.org/10.3892/mmr.2017.7339
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