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Endoplasmic reticulum stress induced by lipopolysaccharide is involved in the association between inflammation and autophagy in INS‑1 cells

  • Authors:
    • Han Liu
    • Jia‑Jing Yin
    • Ming‑Ming Cao
    • Guo‑Dong Liu
    • Ying Su
    • Yan‑Bo Li
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5787-5792
    |
    Published online on: August 24, 2017
       https://doi.org/10.3892/mmr.2017.7350
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Abstract

Type 2 diabetes is a chronic inflammatory disease. Autophagy, the dynamic process of lysosomal degradation of damaged organelles and proteins, may protect β‑cells from destruction by inflammation in type 2 diabetes. The present study investigated the role of autophagy, inflammation and endoplasmic reticulum (ER) stress in type 2 diabetes. INS‑1 cells were incubated with lipopolysaccharide. The chemical chaperone 4‑phenylbutyric acid was used to inhibit ER stress, and 3‑methyadenine (3‑MA) was used to inhibit autophagy. Apoptosis was detected by flow cytometry and cell proliferation using Cell Counting kit‑8 solution. Light chain‑3B, interleukin (IL) 1β, caspase‑1 and C/EBP homologous protein production were assessed by western blotting, and rat activating transcription factor 4 and rat binding immunoglobulin heavy chain protein gene expression were determined by real‑time reverse transcription‑polymerase chain reaction. The results showed that inhibiting autophagy with 3‑MA unexpectedly contributed to cell death in β‑cells. This response was associated with an increase in inflammatory cytokines, including IL1β and caspase‑1. Inhibiting ER stress with 4‑phenylbutyric acid led to a decrease in cell apoptosis. These results showed that autophagy may have a protective effect by reducing inflammatory cytokines in β‑cells. In addition, the inositol‑requiring enzyme 1 pathway mediated the ER stress associated with autophagy and inflammatory cytokines (IL1β and caspase‑1). Therefore, inflammatory cytokines may be critical signalling nodes, which are associated with ER stress‑mediated β‑cell death.
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Copy and paste a formatted citation
Spandidos Publications style
Liu H, Yin JJ, Cao MM, Liu GD, Su Y and Li YB: Endoplasmic reticulum stress induced by lipopolysaccharide is involved in the association between inflammation and autophagy in INS‑1 cells. Mol Med Rep 16: 5787-5792, 2017.
APA
Liu, H., Yin, J., Cao, M., Liu, G., Su, Y., & Li, Y. (2017). Endoplasmic reticulum stress induced by lipopolysaccharide is involved in the association between inflammation and autophagy in INS‑1 cells. Molecular Medicine Reports, 16, 5787-5792. https://doi.org/10.3892/mmr.2017.7350
MLA
Liu, H., Yin, J., Cao, M., Liu, G., Su, Y., Li, Y."Endoplasmic reticulum stress induced by lipopolysaccharide is involved in the association between inflammation and autophagy in INS‑1 cells". Molecular Medicine Reports 16.5 (2017): 5787-5792.
Chicago
Liu, H., Yin, J., Cao, M., Liu, G., Su, Y., Li, Y."Endoplasmic reticulum stress induced by lipopolysaccharide is involved in the association between inflammation and autophagy in INS‑1 cells". Molecular Medicine Reports 16, no. 5 (2017): 5787-5792. https://doi.org/10.3892/mmr.2017.7350
Copy and paste a formatted citation
x
Spandidos Publications style
Liu H, Yin JJ, Cao MM, Liu GD, Su Y and Li YB: Endoplasmic reticulum stress induced by lipopolysaccharide is involved in the association between inflammation and autophagy in INS‑1 cells. Mol Med Rep 16: 5787-5792, 2017.
APA
Liu, H., Yin, J., Cao, M., Liu, G., Su, Y., & Li, Y. (2017). Endoplasmic reticulum stress induced by lipopolysaccharide is involved in the association between inflammation and autophagy in INS‑1 cells. Molecular Medicine Reports, 16, 5787-5792. https://doi.org/10.3892/mmr.2017.7350
MLA
Liu, H., Yin, J., Cao, M., Liu, G., Su, Y., Li, Y."Endoplasmic reticulum stress induced by lipopolysaccharide is involved in the association between inflammation and autophagy in INS‑1 cells". Molecular Medicine Reports 16.5 (2017): 5787-5792.
Chicago
Liu, H., Yin, J., Cao, M., Liu, G., Su, Y., Li, Y."Endoplasmic reticulum stress induced by lipopolysaccharide is involved in the association between inflammation and autophagy in INS‑1 cells". Molecular Medicine Reports 16, no. 5 (2017): 5787-5792. https://doi.org/10.3892/mmr.2017.7350
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