Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
November-2017 Volume 16 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2017 Volume 16 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Amplification by (‑)‑epigallocatechin gallate of prostaglandin F2α‑stimulated synthesis of osteoprotegerin in osteoblasts

  • Authors:
    • Go Sakai
    • Takanobu Otsuka
    • Kazuhiko Fujita
    • Shingo Kainuma
    • Gen Kuroyanagi
    • Tetsu Kawabata
    • Rie Matsushima‑Nishiwaki
    • Osamu Kozawa
    • Haruhiko Tokuda
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya 467‑8601, Japan, Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu 501‑1194, Japan
  • Pages: 6376-6381
    |
    Published online on: August 24, 2017
       https://doi.org/10.3892/mmr.2017.7354
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

(‑)‑Epigallocatechin gallate (EGCG) and chlorogenic acid (CGA), major flavonoids in green tea, and coffee, respectively, are recognized as possessing potential benefits in a multitude of human health conditions, including bone disorders. We have previously demonstrated that prostaglandin F2α (PGF2α), a potent bone remodeling mediator, stimulates the synthesis of osteoprotegerin (OPG) through the activation of p44/p42 mitogen‑activated protein kinase (MAPK), p38 MAPK and stress activated protein kinase/c‑Jun N‑terminal kinase (SAPK/JNK) in osteoblast‑like MC3T3‑E1 cells. In the present study, the effects of EGCG and CGA on PGF2α‑stimulated OPG synthesis in MC3T3‑E1 cells were investigated. EGCG significantly upregulated PGF2α‑stimulated OPG release, whereas CGA did not affect OPG release. The PGF2α‑induced expression level of OPG mRNA was enhanced by EGCG. Regarding the intracellular signaling underlying the effect of EGCG, EGCG failed to affect PGF2α‑stimulated phosphorylation of p44/p42 MAPK, p38 MAPK or SAPK/JNK. EGCG by itself markedly induced the phosphorylation of p44/p42 MAP kinase for up to 10 min and the status decreased subsequently, whereas EGCG did not significantly affect the phosphorylation status of p38 MAPK or SAPK/JNK within 60 min. These results indicated that EGCG, but not CGA amplifies the PGF2α‑stimulated OPG synthesis in osteoblasts.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Thielecke F and Boschmann M: The potential role of green tea catechins in the prevention of the metabolic syndrome-a review. Phytochemistry. 70:11–24. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Shimizu M, Adachi S, Masuda M, Kozawa O and Moriwaki H: Cancer chemoprevention with green tea catechins by targeting receptor tyrosine kinases. Mol Nutr Food Res. 55:832–843. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Higdon JV and Frei B: Coffee and health: A review of recent human research. Crit Rev Food Sci Nutr. 46:101–123. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Harborne JB and Williams CA: Advances in flavonoid research since 1992. Phytochemistry. 55:481–504. 2000. View Article : Google Scholar : PubMed/NCBI

5 

George SE, Ramalakshmi K and Rao LJ Mohan: A perception on health benefits of coffee. Crit Rev Food Sci Nutr. 48:464–486. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Shen CL, Yeh JK, Cao JJ and Wang JS: Green tea and bone metabolism. Nutr Res. 29:437–456. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Karsenty G and Oury F: Biology without walls: The novel endocrinology of bone. Annu Rev Physiol. 74:87–105. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Parfitt AM: Targeted and nontargeted bone remodeling: Relationship to basic multicellular unit origination and progression. Bone. 30:5–7. 2002. View Article : Google Scholar : PubMed/NCBI

9 

Kwak SC, Lee C, Kim JY, Oh HM, So HS, Lee MS, Rho MC and Oh J: Chlorogenic acid inhibits osteoclast differentiation and bone resorption by down-regulation of receptor activator of nuclear factor kappa-B ligand-induced nuclear factor of activated T cells c1 expression. Biol Pharm Bull. 36:1779–1786. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Folwarczna J, Pytlik M, Zych M, Cegiela U, Nowinska B, Kaczmarczyk-Sedlak L, Sliwinski L, Trzeciak H and Trzeciak HI: Effects of caffeic and chlorogenic acids on the rat skeletal system. Eur Rev Med Pharmacol Sci. 19:682–693. 2015.PubMed/NCBI

11 

Takai S, Matsushima-Nishiwaki R, Adachi S, Natsume H, Minamitani C, Mizutani J, Otsuka T, Tokuda H and Kozawa O: (−)-Epigallocatechin gallate reduces platelet-derived growth factor-BB-stimulated interleukin-6 synthesis in osteoblasts: Suppression of SAPK/JNK. Mediators Inflamm. 2008:2918082008. View Article : Google Scholar : PubMed/NCBI

12 

Tokuda H, Takai S, Hanai Y, Matsushima-Nishiwaki R, Yamauchi J, Harada A, Hosoi T, Ohta T and Kozawa O: (−)-Epigallocatechin gallate inhibits basic fibroblast growth factor-stimulated interleukin-6 synthesis in osteoblasts. Horm Metab Res. 40:674–678. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Tokuda H, Takai S, Hanai Y, Matsushima-Nishiwaki R, Hosoi T, Harada A, Ohta T and Kozawa O: (−)-Epigallocatechin gallate suppresses endothelin-1-induced interleukin-6 synthesis in osteoblasts: inhibition of p44/p42 MAP kinase activation. FEBS Lett. 581:1311–1316. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Yamamoto N, Tokuda H, Kuroyanagi G, Kainuma S, Ohguchi R, Fujita K, Matsushima-Nishiwaki R, Kozawa O and Otsuka T: Amplification by (−)-epigallocatechin gallate and chlorogenic acid of TNF-α-stimulated interleukin-6 synthesis in osteoblasts. Int J Mol Med. 36:1707–1712. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Kostenuik PJ and Shalhoub V: Osteoprotegerin: A physiological and pharmacological inhibitor of bone resorption. Curr Pharm Des. 7:613–635. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Simonet WS, Lacey DL, Dunstan CR, Kelley M, Chang MS, Lüthy R, Nguyen HQ, Wooden S, Bennett L, Boone T, et al: Osteoprotegerin: A novel secreted protein involved in the regulation of bone density. Cell. 89:309–319. 1997. View Article : Google Scholar : PubMed/NCBI

17 

Mizuno A, Amizuka N, Irie K, Murakami A, Fujise N, Kanno T, Sato Y, Nakagawa N, Yasuda H, Mochizuki S, et al: Severe osteoporosis in mice lacking osteoclastogenesis inhibitory factor/osteoprotegerin. Biochem Biophys Res Commun. 247:610–615. 1998. View Article : Google Scholar : PubMed/NCBI

18 

Mizuno A, Kanno T, Hoshi M, Shibata O, Yano K, Fujise N, Kinosaki M, Yamaguchi K, Tsuda E, Murakami A, et al: Transgenic mice overexpressing soluble osteoclast differentiation factor (sODF) exhibit severe osteoporosis. J Bone Miner Metab. 20:337–344. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Tat S Kwan, Padrines M, Théoleyre S, Heymann D and Fortun Y: IL-6, RANKL, TNF-α/IL-1: Interrelations in bone resorption pathophysiology. Cytokine Growth Factor Rev. 15:49–60. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Hikiji H, Takato T, Shimizu T and Ishii S: The roles of prostanoids, leukotrienes, and platelet-activating factor in bone metabolism and disease. Prog Lipid Res. 47:107–126. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Agas D, Marchetti L, Hurley MM and Sabbieti MG: Prostaglandin F2α: A bone remodeling mediator. J Cell Physiol. 228:25–29. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Kuroyanagi G, Tokuda H, Matsushima-Nishiwaki R, Kondo A, Mizutani J, Kozawa O and Otsuka T: Resveratrol suppresses prostaglandin F2α-induced osteoprotegerin synthesis in osteoblasts: Inhibition of the MAP kinase signaling. Arch Biochem Biophys. 542:39–45. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Sudo H, Kodama HA, Amagai Y, Yamamoto S and Kasai S: In vivo differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol. 96:191–198. 1983. View Article : Google Scholar : PubMed/NCBI

24 

Kozawa O, Tokuda H, Miwa M, Kotoyori J and Oiso Y: Cross-talk regulation between cyclic AMP production and phosphoinositide hydrolysis induced by prostaglandin E2 in osteoblast-like cells. Exp Cell Res. 198:130–134. 1992. View Article : Google Scholar : PubMed/NCBI

25 

Simpson DA, Feeney S, Boyle C and Stitt AW: Retinal VEGF mRNA measured by SYBR green I fluorescence: A versatile approach to quantitative PCR. Mol Vis. 6:178–183. 2000.PubMed/NCBI

26 

Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680–685. 1970. View Article : Google Scholar : PubMed/NCBI

27 

Kato K, Ito H, Hasegawa K, Inaguma Y, Kozawa O and Asano T: Modulation of the stress-induced synthesis of hsp27 and alpha B-crystallin by cyclic AMP in C6 rat glioma cells. J Neurochem. 66:946–950. 1996. View Article : Google Scholar : PubMed/NCBI

28 

Kyriakis JM and Avruch J: Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation. Physiol Rev. 81:807–869. 2001.PubMed/NCBI

29 

Widmann C, Gibson S, Jarpe MB and Johnson GL: Mitogen-activated protein kinase: Conservation of a three-kinase module from yeast to human. Physiol Rev. 79:143–180. 1999.PubMed/NCBI

30 

Dhanasekaran N and Reddy E Premkumar: Signaling by dual specificity kinases. Oncogene. 17:1447–1455. 1998. View Article : Google Scholar : PubMed/NCBI

31 

Lin A, Minden A, Martinetto H, Claret FX, Lange-Carter C, Mercurio F, Johnson GL and Karin M: Identification of a dual specificity kinase that activates the Jun kinases and p38-Mpk2. Science. 14:286–290. 1995. View Article : Google Scholar

32 

Greenfield EM, Bi Y and Miyauchi A: Regulation of osteoclast activity. Life Sci. 65:1087–1102. 1999. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Sakai G, Otsuka T, Fujita K, Kainuma S, Kuroyanagi G, Kawabata T, Matsushima‑Nishiwaki R, Kozawa O and Tokuda H: Amplification by (‑)‑epigallocatechin gallate of prostaglandin F2α‑stimulated synthesis of osteoprotegerin in osteoblasts. Mol Med Rep 16: 6376-6381, 2017.
APA
Sakai, G., Otsuka, T., Fujita, K., Kainuma, S., Kuroyanagi, G., Kawabata, T. ... Tokuda, H. (2017). Amplification by (‑)‑epigallocatechin gallate of prostaglandin F2α‑stimulated synthesis of osteoprotegerin in osteoblasts. Molecular Medicine Reports, 16, 6376-6381. https://doi.org/10.3892/mmr.2017.7354
MLA
Sakai, G., Otsuka, T., Fujita, K., Kainuma, S., Kuroyanagi, G., Kawabata, T., Matsushima‑Nishiwaki, R., Kozawa, O., Tokuda, H."Amplification by (‑)‑epigallocatechin gallate of prostaglandin F2α‑stimulated synthesis of osteoprotegerin in osteoblasts". Molecular Medicine Reports 16.5 (2017): 6376-6381.
Chicago
Sakai, G., Otsuka, T., Fujita, K., Kainuma, S., Kuroyanagi, G., Kawabata, T., Matsushima‑Nishiwaki, R., Kozawa, O., Tokuda, H."Amplification by (‑)‑epigallocatechin gallate of prostaglandin F2α‑stimulated synthesis of osteoprotegerin in osteoblasts". Molecular Medicine Reports 16, no. 5 (2017): 6376-6381. https://doi.org/10.3892/mmr.2017.7354
Copy and paste a formatted citation
x
Spandidos Publications style
Sakai G, Otsuka T, Fujita K, Kainuma S, Kuroyanagi G, Kawabata T, Matsushima‑Nishiwaki R, Kozawa O and Tokuda H: Amplification by (‑)‑epigallocatechin gallate of prostaglandin F2α‑stimulated synthesis of osteoprotegerin in osteoblasts. Mol Med Rep 16: 6376-6381, 2017.
APA
Sakai, G., Otsuka, T., Fujita, K., Kainuma, S., Kuroyanagi, G., Kawabata, T. ... Tokuda, H. (2017). Amplification by (‑)‑epigallocatechin gallate of prostaglandin F2α‑stimulated synthesis of osteoprotegerin in osteoblasts. Molecular Medicine Reports, 16, 6376-6381. https://doi.org/10.3892/mmr.2017.7354
MLA
Sakai, G., Otsuka, T., Fujita, K., Kainuma, S., Kuroyanagi, G., Kawabata, T., Matsushima‑Nishiwaki, R., Kozawa, O., Tokuda, H."Amplification by (‑)‑epigallocatechin gallate of prostaglandin F2α‑stimulated synthesis of osteoprotegerin in osteoblasts". Molecular Medicine Reports 16.5 (2017): 6376-6381.
Chicago
Sakai, G., Otsuka, T., Fujita, K., Kainuma, S., Kuroyanagi, G., Kawabata, T., Matsushima‑Nishiwaki, R., Kozawa, O., Tokuda, H."Amplification by (‑)‑epigallocatechin gallate of prostaglandin F2α‑stimulated synthesis of osteoprotegerin in osteoblasts". Molecular Medicine Reports 16, no. 5 (2017): 6376-6381. https://doi.org/10.3892/mmr.2017.7354
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team