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Article Open Access

Neuroprotective effects of hydrogen sulfide on sodium azide‑induced autophagic cell death in PC12 cells

  • Authors:
    • Haiyan Shan
    • Yang Chu
    • Pan Chang
    • Lijun Yang
    • Yi Wang
    • Shaohua Zhu
    • Mingyang Zhang
    • Luyang Tao
  • View Affiliations / Copyright

    Affiliations: Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, Shanghai 200063, P.R. China, Institute of Forensic Sciences, Soochow University, Suzhou, Jiangsu 215123, P.R. China, Central Laboratory, Second Affiliated Hospital of Xi'an Medical College, Xi'an, Shaanxi 710038, P.R. China
    Copyright: © Shan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5938-5946
    |
    Published online on: August 25, 2017
       https://doi.org/10.3892/mmr.2017.7363
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Abstract

Sodium azide (NaN3) is a chemical of rapidly growing commercial importance. It is very acutely toxic and inhibits cytochrome oxidase (COX) by binding irreversibly to the heme cofactor. A previous study from our group demonstrated that hydrogen sulfide (H2S), the third endogenous gaseous mediator identified, had protective effects against neuronal damage induced by traumatic brain injury (TBI). It is well‑known that TBI can reduce the activity of COX and have detrimental effects on the central nervous system metabolism. Therefore, in the present study, it was hypothesized that H2S may provide neuroprotection against NaN3 toxicity. The current results revealed that NaN3 treatment induced non‑apoptotic cell death, namely autophagic cell death, in PC12 cells. Expression of the endogenous H2S‑producing enzymes, cystathionine‑β‑synthase and 3‑mercaptopyruvate sulfurtransferase, decreased in a dose‑dependent manner following NaN3 treatment. Pretreatment with H2S markedly attenuated the NaN3‑induced cell viability loss and autophagic cell death in a dose‑dependent manner. The present study suggests that H2S‑based strategies may have future potential in the prevention and/or therapy of neuronal damage following NaN3 exposure.
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Copy and paste a formatted citation
Spandidos Publications style
Shan H, Chu Y, Chang P, Yang L, Wang Y, Zhu S, Zhang M and Tao L: Neuroprotective effects of hydrogen sulfide on sodium azide‑induced autophagic cell death in PC12 cells. Mol Med Rep 16: 5938-5946, 2017.
APA
Shan, H., Chu, Y., Chang, P., Yang, L., Wang, Y., Zhu, S. ... Tao, L. (2017). Neuroprotective effects of hydrogen sulfide on sodium azide‑induced autophagic cell death in PC12 cells. Molecular Medicine Reports, 16, 5938-5946. https://doi.org/10.3892/mmr.2017.7363
MLA
Shan, H., Chu, Y., Chang, P., Yang, L., Wang, Y., Zhu, S., Zhang, M., Tao, L."Neuroprotective effects of hydrogen sulfide on sodium azide‑induced autophagic cell death in PC12 cells". Molecular Medicine Reports 16.5 (2017): 5938-5946.
Chicago
Shan, H., Chu, Y., Chang, P., Yang, L., Wang, Y., Zhu, S., Zhang, M., Tao, L."Neuroprotective effects of hydrogen sulfide on sodium azide‑induced autophagic cell death in PC12 cells". Molecular Medicine Reports 16, no. 5 (2017): 5938-5946. https://doi.org/10.3892/mmr.2017.7363
Copy and paste a formatted citation
x
Spandidos Publications style
Shan H, Chu Y, Chang P, Yang L, Wang Y, Zhu S, Zhang M and Tao L: Neuroprotective effects of hydrogen sulfide on sodium azide‑induced autophagic cell death in PC12 cells. Mol Med Rep 16: 5938-5946, 2017.
APA
Shan, H., Chu, Y., Chang, P., Yang, L., Wang, Y., Zhu, S. ... Tao, L. (2017). Neuroprotective effects of hydrogen sulfide on sodium azide‑induced autophagic cell death in PC12 cells. Molecular Medicine Reports, 16, 5938-5946. https://doi.org/10.3892/mmr.2017.7363
MLA
Shan, H., Chu, Y., Chang, P., Yang, L., Wang, Y., Zhu, S., Zhang, M., Tao, L."Neuroprotective effects of hydrogen sulfide on sodium azide‑induced autophagic cell death in PC12 cells". Molecular Medicine Reports 16.5 (2017): 5938-5946.
Chicago
Shan, H., Chu, Y., Chang, P., Yang, L., Wang, Y., Zhu, S., Zhang, M., Tao, L."Neuroprotective effects of hydrogen sulfide on sodium azide‑induced autophagic cell death in PC12 cells". Molecular Medicine Reports 16, no. 5 (2017): 5938-5946. https://doi.org/10.3892/mmr.2017.7363
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