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Article

Screening and validation of differentially expressed extracellular miRNAs in acute pancreatitis

  • Authors:
    • Shishuai Meng
    • Hao Wang
    • Dongbo Xue
    • Weihui Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Critical Care Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
  • Pages: 6412-6418
    |
    Published online on: August 28, 2017
       https://doi.org/10.3892/mmr.2017.7374
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Abstract

The present study aimed to screen for differentially expressed extracellular microRNAs (miRNAs) during the development of acute pancreatitis (AP) and validate the miRNA expression in the plasma of patients with AP. The culture medium of taurolithocholic acid‑3 sulfate‑treated rat pancreatic acinar AR42J cells was collected to extract total RNA for miRNA microarray analysis. Compared with the miRNA test results of the AP rats in the GEO databases, the differentially expressed extracellular miRNAs were screened. The TargetScan, miRanda, and PicTar programs were used for target gene prediction of the identified miRNAs, and gene ontology‑biological processes (GO‑BP) functional annotation was performed. Finally, the results from the combined microarray analyses (in vitro cell line and in vivo rat samples) were validated using plasma samples from patients with mild and moderately severe AP by reverse transcription‑polymerase chain reaction. The results demonstrated that extracellular miR‑24 was differentially expressed by microarray and bioinformatics analysis in both the cell line and the animal model of AP. Bioinformatics prediction analysis revealed that downstream target genes of miR‑24 included Vav2, Syk, Lhcgr, Slc9a3r1, Cacnb1, Cacna1b, Bcl10, and Fgd3. Functional enrichment analysis revealed that the main GO‑BP predicted functional presentations were positive regulation of calcium‑mediated signaling, activation of c‑Jun N‑terminal kinase activity, calcium ion transport, regulation of Rho protein signal transduction, negative regulation of the protein kinase B signaling cascade, and the T cell receptor signaling pathway. Validation analysis for the plasma miR‑24 expression in humans revealed a significant upregulation of miR‑24 in the plasma samples of AP patients compared with the healthy controls, while no significant difference was observed in the miR‑24 expression between the mild and the moderately severe AP groups. The present study confirmed the high expression of miR‑24 in peripheral blood during AP, suggesting that miR‑24 might have an intercellular communication role contributing to the AP‑associated distant organ injury.
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1 

An F, Zhan Q, Xia M, Jiang L, Lu G, Huang M, Guo J and Liu S: From moderately severe to severe hypertriglyceridemia induced acute pancreatitis: Circulating miRNAs play role as potential biomarkers. PLoS One. 9:e1110582014. View Article : Google Scholar : PubMed/NCBI

2 

Goodwin D, Rosenzweig B, Zhang J, Xu L, Stewart S, Thompson K and Rouse R: Evaluation of miR-216a and miR-217 as potential biomarkers of acute pancreatic injury in rats and mice. Biomarkers. 19:517–529. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Qin T, Fu Q, Pan YF, Liu CJ, Wang YZ, Hu MX, Tang Q and Zhang HW: Expressions of miR-22 and miR-135a in acute pancreatitis. J Huazhong Univ Sci Technolog Med Sci. 34:225–233. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Huan C, Kim D, Ou P, Alfonso A and Stanek A: Mechanisms of interleukin-22′s beneficial effects in acute pancreatitis. World J Gastrointest Pathophysiol. 7:108–116. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Liang X, Zhang B, Chen Q, Zhang J, Lei B, Li B, Wei Y, Zhai R, Liang Z, He S and Tang B: The mechanism underlying alpinetin-mediated alleviation of pancreatitis-associated lung injury through upregulating aquaporin-1. Drug Des Devel Ther. 10:841–850. 2016.PubMed/NCBI

6 

Shi C, Andersson R, Zhao X and Wang X: Potential role of reactive oxygen species in pancreatitis-associated multiple organ dysfunction. Pancreatology. 5:492–500. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Araki Y, Andoh A, Yokono T, Asano N, Yoshikawa K, Bamba S, Ishizuka I and Fujiyama Y: The free radical scavenger edaravone suppresses experimental closed duodenal loop-induced acute pancreatitis in rats. Int J Mol Med. 12:121–124. 2003.PubMed/NCBI

8 

Wang H, Jiang Y, Lu M, Sun B, Qiao X, Xue D and Zhang W: STX12 lncRNA/miR-148a/SMAD5 participate in the regulation of pancreatic stellate cell activation through a mechanism involving competing endogenous RNA. Pancreatology. 17:237–246. 2017. View Article : Google Scholar : PubMed/NCBI

9 

Zhao Y, Wang H, Lu M, Qiao X, Sun B, Zhang W and Xue D: Pancreatic acinar cells employ miRNAs as mediators of intercellular communication to participate in the regulation of pancreatitis-associated macrophage activation. Mediators Inflamm. 2016:63404572016. View Article : Google Scholar : PubMed/NCBI

10 

Bolstad BM, Irizarry RA, Astrand M and Speed TP: A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics. 19:185–193. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Blenkiron C, Askelund KJ, Shanbhag ST, Chakraborty M, Petrov MS, Delahunt B, Windsor JA and Phillips AR: MicroRNAs in mesenteric lymph and plasma during acute pancreatitis. Ann Surg. 260:341–347. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, et al: Gene ontology: Tool for the unification of biology. The Gene Ontology Consortium. Nat Genet. 25:25–29. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, Amin N, Schwikowski B and Ideker T: Cytoscape: A software environment for integrated models of biomolecular interaction networks. Genome Res. 13:2498–2504. 2003. View Article : Google Scholar : PubMed/NCBI

14 

Jiao X, Sherman BT, da W Huang, Stephens R, Baseler MW, Lane HC and Lempicki RA: DAVID-WS: A stateful web service to facilitate gene/protein list analysis. Bioinformatics. 28:1805–1806. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Balthazar EJ: Acute pancreatitis: Assessment of severity with clinical and CT evaluation. Radiology. 223:603–613. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Council for International Organizations of Medical Sciences, . International ethical guidelines for biomedical research involving human subjects. Bull Med Ethics. 1–23. 2002.

17 

Gao X, Gulari E and Zhou X: In situ synthesis of oligonucleotide microarrays. Biopolymers. 73:579–596. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Kang X, Wang LZ, Wang YG, Liu L, Fan ZW, Bai LZ and Lu XG: Expression and significance of phosphatidylinositol 3-kinase/protein kinase B signal transduction pathway in severe acute pancreatitis-associated lung injury. Zhonghua Yi Xue Za Zhi. 90:732–737. 2010.(In Chinese). PubMed/NCBI

19 

Usborne AL, Smith AT, Engle SK, Watson DE, Sullivan JM and Walgren JL: Biomarkers of exocrine pancreatic injury in 2 rat acute pancreatitis models. Toxicol Pathol. 42:195–203. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Lee EJ, Gusev Y, Jiang J, Nuovo GJ, Lerner MR, Frankel WL, Morgan DL, Postier RG, Brackett DJ and Schmittgen TD: Expression profiling identifies microRNA signature in pancreatic cancer. Int J Cancer. 120:1046–1054. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Kuśnierz-Cabala B, Nowak E, Sporek M, Kowalik A, Kuźniewski M, Enguita FJ and Stepien E: Serum levels of unique miR-551-5p and endothelial-specific miR-126a-5p allow discrimination of patients in the early phase of acute pancreatitis. Pancreatology. 15:344–351. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Jeyaseelan K, Lim KY and Armugam A: MicroRNA expression in the blood and brain of rats subjected to transient focal ischemia by middle cerebral artery occlusion. Stroke. 39:959–966. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Hamada S, Masamune A, Kanno A and Shimosegawa T: Comprehensive analysis of serum microRNAs in autoimmune pancreatitis. Digestion. 91:263–271. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Di Gregoli K, Jenkins N, Salter R, White S, Newby AC and Johnson JL: MicroRNA-24 regulates macrophage behavior and retards atherosclerosis. Arterioscler Thromb Vasc Biol. 34:1990–2000. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Katoh M: Therapeutics targeting angiogenesis: Genetics and epigenetics, extracellular miRNAs and signaling networks (Review). Int J Mol Med. 32:763–767. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Lakkaraju A and Rodriguez-Boulan E: Itinerant exosomes: Emerging roles in cell and tissue polarity. Trends Cell Biol. 18:199–209. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Zhang Y, Wu XH, Luo CL, Zhang JM, He BC and Chen G: Interleukin-12-anchored exosomes increase cytotoxicity of T lymphocytes by reversing the JAK/STAT pathway impaired by tumor-derived exosomes. Int J Mol Med. 25:695–700. 2010.PubMed/NCBI

28 

Valadi H, Ekström K, Bossios A, Sjostrand M, Lee JJ and Lötvall JO: Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol. 9:654–659. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Hunter MP, Ismail N, Zhang X, Aguda BD, Lee EJ, Yu L, Xiao T, Schafer J, Lee ML, Schmittgen TD, et al: Detection of microRNA expression in human peripheral blood microvesicles. PLoS One. 3:e36942008. View Article : Google Scholar : PubMed/NCBI

30 

Kylänpää ML, Repo H and Puolakkainen PA: Inflammation and immunosuppression in severe acute pancreatitis. World J Gastroenterol. 16:2867–2872. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Maegdefessel L, Spin JM, Raaz U, Eken SM, Toh R, Azuma J, Adam M, Nakagami F, Heymann HM, Chernogubova E, et al: miR-24 limits aortic vascular inflammation and murine abdominal aneurysm development. Nat Commun. 5:52142014. View Article : Google Scholar : PubMed/NCBI

32 

Fanous MY, Phillips AJ and Windsor JA: Mesenteric lymph: The bridge to future management of critical illness. JOP. 8:374–399. 2007.PubMed/NCBI

33 

Flint RS, Phillips AR, Power SE, Dunbar PR, Brown C, Delahunt B, Cooper GJ and Windsor JA: Acute pancreatitis severity is exacerbated by intestinal ischemia-reperfusion conditioned mesenteric lymph. Surgery. 143:404–413. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Endo K, Weng H, Kito N, Fukushima Y and Iwai N: MiR-216a and miR-216b as markers for acute phased pancreatic injury. Biomed Res. 34:179–188. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Kylanpaa L, Rakonczay Z Jr and O'Reilly DA: The clinical course of acute pancreatitis and the inflammatory mediators that drive it. Int J Inflam. 2012:3606852012. View Article : Google Scholar : PubMed/NCBI

36 

Ohmoto K and Yamamoto S: Serum interleukin-6 and interleukin-10 in patients with acute pancreatitis: Clinical implications. Hepatogastroenterology. 52:990–994. 2005.PubMed/NCBI

37 

Liu Y, Zhou D, Long FW, Chen KL, Yang HW, Lv ZY, Zhou B, Peng ZH, Sun XF, Li Y and Zhou ZG: Resolvin D1 protects against inflammation in experimental acute pancreatitis and associated lung injury. Am J Physiol Gastrointest Liver Physiol. 310:G303–G309. 2016. View Article : Google Scholar : PubMed/NCBI

38 

Tran TH, Krishnan S and Amiji MM: MicroRNA-223 induced repolarization of peritoneal macrophages using CD44 targeting hyaluronic acid nanoparticles for anti-inflammatory effects. PLoS One. 11:e01520242016. View Article : Google Scholar : PubMed/NCBI

39 

Curtale G, Mirolo M, Renzi TA, Rossato M, Bazzoni F and Locati M: Negative regulation of Toll-like receptor 4 signaling by IL-10-dependent microRNA-146b. Proc Natl Acad Sci USA. 110:11499–11504. 2013; View Article : Google Scholar : PubMed/NCBI

40 

Awla D, Hartman H, Abdulla A, Zhang S, Rahman M, Regnèr S and Thorlacius H: Rho-kinase signalling regulates trypsinogen activation and tissue damage in severe acute pancreatitis. Br J Pharmacol. 162:648–658. 2011. View Article : Google Scholar : PubMed/NCBI

41 

Bae GS, Heo KH, Park KC, Choi SB, Jo IJ, Seo SH, Kim DG, Shin JY, Kang DG, Lee HS, et al: Apamin attenuated cerulein-induced acute pancreatitis by inhibition of JNK pathway in mice. Dig Dis Sci. 58:2908–2917. 2013. View Article : Google Scholar : PubMed/NCBI

42 

Ramnath RD, Sun J and Bhatia M: Role of calcium in substance P-induced chemokine synthesis in mouse pancreatic acinar cells. Br J Pharmacol. 154:1339–1348. 2008. View Article : Google Scholar : PubMed/NCBI

43 

Xue P, Deng LH, Zhang ZD, Yang XN, Xia Q, Xiang DK, Huang L and Wan MH: Effect of Chaiqinchengqi decoction on sarco/endoplasmic reticulum Ca2+-ATPase mRNA expression of pancreatic tissues in acute pancreatitis rats. World J Gastroenterol. 14:2343–2348. 2008. View Article : Google Scholar : PubMed/NCBI

44 

Fordham JB, Naqvi AR and Nares S: miR-24 regulates macrophage polarization and plasticity. J Clin Cell Immunol. 6:pii:3622015.

45 

Zhu Y, You W, Wang H, Li Y, Qiao N, Shi Y, Zhang C, Bleich D and Han X: MicroRNA-24/MODY gene regulatory pathway mediates pancreatic β-cell dysfunction. Diabetes. 62:3194–3206. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Meng S, Wang H, Xue D and Zhang W: Screening and validation of differentially expressed extracellular miRNAs in acute pancreatitis. Mol Med Rep 16: 6412-6418, 2017.
APA
Meng, S., Wang, H., Xue, D., & Zhang, W. (2017). Screening and validation of differentially expressed extracellular miRNAs in acute pancreatitis. Molecular Medicine Reports, 16, 6412-6418. https://doi.org/10.3892/mmr.2017.7374
MLA
Meng, S., Wang, H., Xue, D., Zhang, W."Screening and validation of differentially expressed extracellular miRNAs in acute pancreatitis". Molecular Medicine Reports 16.5 (2017): 6412-6418.
Chicago
Meng, S., Wang, H., Xue, D., Zhang, W."Screening and validation of differentially expressed extracellular miRNAs in acute pancreatitis". Molecular Medicine Reports 16, no. 5 (2017): 6412-6418. https://doi.org/10.3892/mmr.2017.7374
Copy and paste a formatted citation
x
Spandidos Publications style
Meng S, Wang H, Xue D and Zhang W: Screening and validation of differentially expressed extracellular miRNAs in acute pancreatitis. Mol Med Rep 16: 6412-6418, 2017.
APA
Meng, S., Wang, H., Xue, D., & Zhang, W. (2017). Screening and validation of differentially expressed extracellular miRNAs in acute pancreatitis. Molecular Medicine Reports, 16, 6412-6418. https://doi.org/10.3892/mmr.2017.7374
MLA
Meng, S., Wang, H., Xue, D., Zhang, W."Screening and validation of differentially expressed extracellular miRNAs in acute pancreatitis". Molecular Medicine Reports 16.5 (2017): 6412-6418.
Chicago
Meng, S., Wang, H., Xue, D., Zhang, W."Screening and validation of differentially expressed extracellular miRNAs in acute pancreatitis". Molecular Medicine Reports 16, no. 5 (2017): 6412-6418. https://doi.org/10.3892/mmr.2017.7374
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