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Article

Analysis of the Panax ginseng stem/leaf transcriptome and gene expression during the leaf expansion period

  • Authors:
    • Shichao Liu
    • Meichen Liu
    • Siming Wang
    • Yanling Lin
    • Hui Zhang
    • Qun Wang
    • Yu Zhao
  • View Affiliations / Copyright

    Affiliations: Center of Chinese Medicine and Bio‑Engineering Research, Changchun University of Chinese Medicine, Changchun, Jilin 130000, P.R. China, Science and Education Department, Jilin Provincial Academy of Chinese Medicine Sciences, Changchun, Jilin 130000, P.R. China
  • Pages: 6396-6404
    |
    Published online on: August 29, 2017
       https://doi.org/10.3892/mmr.2017.7377
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Abstract

Ginseng (Panax ginseng C.A Meyer) is a widely used herbal remedy, however, the majority of studies have focused on the roots, with less known about the aerial regions of the plant. As the stems and leaves are the primary aerial tissues, the present study characterized their transcriptional profiles using Illumina next‑generation sequencing technology. The gene expression profiles and the functional genes of ginseng stems (GS) and leaves (GL) were analyzed during the leaf‑expansion period. cDNA libraries of the GS and GL of 5‑year‑old ginseng plants were separately constructed. In the GS library, 38,000,000 sequencing reads were produced. These reads were assembled into 99,809 unique sequences with a mean size of 572 bp, and 57,371 sequences were identified based on similarity searches against known proteins. The assembled sequences were annotated using Gene Ontology terms, Clusters of Orthologous Groups classifications and Kyoto Encyclopedia of Genes and Genomes pathways. For GL, >118,000,000 sequencing reads were produced, which were assembled into 73,163 unique sequences, from which 50,523 sequences were identified. Additionally, several genes involved in the regulation of growth‑related, stress‑related, pathogenesis‑related, and chlorophyll metabolism‑associated proteins were found and expressed at high levels, with low expression levels of ginsenoside biosynthesis enzymes also found. The results of the present study provide a valuable useful sequence resource for ginseng in general, and specifically for further investigations of the functional genomics and molecular genetics of GS and GL during early growth.
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Copy and paste a formatted citation
Spandidos Publications style
Liu S, Liu M, Wang S, Lin Y, Zhang H, Wang Q and Zhao Y: Analysis of the Panax ginseng stem/leaf transcriptome and gene expression during the leaf expansion period. Mol Med Rep 16: 6396-6404, 2017.
APA
Liu, S., Liu, M., Wang, S., Lin, Y., Zhang, H., Wang, Q., & Zhao, Y. (2017). Analysis of the Panax ginseng stem/leaf transcriptome and gene expression during the leaf expansion period. Molecular Medicine Reports, 16, 6396-6404. https://doi.org/10.3892/mmr.2017.7377
MLA
Liu, S., Liu, M., Wang, S., Lin, Y., Zhang, H., Wang, Q., Zhao, Y."Analysis of the Panax ginseng stem/leaf transcriptome and gene expression during the leaf expansion period". Molecular Medicine Reports 16.5 (2017): 6396-6404.
Chicago
Liu, S., Liu, M., Wang, S., Lin, Y., Zhang, H., Wang, Q., Zhao, Y."Analysis of the Panax ginseng stem/leaf transcriptome and gene expression during the leaf expansion period". Molecular Medicine Reports 16, no. 5 (2017): 6396-6404. https://doi.org/10.3892/mmr.2017.7377
Copy and paste a formatted citation
x
Spandidos Publications style
Liu S, Liu M, Wang S, Lin Y, Zhang H, Wang Q and Zhao Y: Analysis of the Panax ginseng stem/leaf transcriptome and gene expression during the leaf expansion period. Mol Med Rep 16: 6396-6404, 2017.
APA
Liu, S., Liu, M., Wang, S., Lin, Y., Zhang, H., Wang, Q., & Zhao, Y. (2017). Analysis of the Panax ginseng stem/leaf transcriptome and gene expression during the leaf expansion period. Molecular Medicine Reports, 16, 6396-6404. https://doi.org/10.3892/mmr.2017.7377
MLA
Liu, S., Liu, M., Wang, S., Lin, Y., Zhang, H., Wang, Q., Zhao, Y."Analysis of the Panax ginseng stem/leaf transcriptome and gene expression during the leaf expansion period". Molecular Medicine Reports 16.5 (2017): 6396-6404.
Chicago
Liu, S., Liu, M., Wang, S., Lin, Y., Zhang, H., Wang, Q., Zhao, Y."Analysis of the Panax ginseng stem/leaf transcriptome and gene expression during the leaf expansion period". Molecular Medicine Reports 16, no. 5 (2017): 6396-6404. https://doi.org/10.3892/mmr.2017.7377
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