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Article

Effect of 1,25(OH)2D3 on high glucose‑induced autophagy inhibition in peritoneum

  • Authors:
    • Lina Yang
    • Yi Fan
    • Xiuli Zhang
    • Jin Liu
    • Jianfei Ma
  • View Affiliations / Copyright

    Affiliations: Department of Geriatrics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Nephrology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
  • Pages: 7080-7085
    |
    Published online on: August 31, 2017
       https://doi.org/10.3892/mmr.2017.7408
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Abstract

High glucose (HG) may damage the structure and function of the peritoneal membrane, and is considered to be one of the most important factors that leads to peritoneal fibrosis and ultrafiltration failure. Recently, 1,25(OH)2D3, the active form of vitamin D, was demonstrated to protect against epithelial‑mesenchymal transition and fibrosis in peritoneal mesothelium and other organs. Accumulating evidence has suggested that autophagy serves a protective role in certain diseases by regulating cell survival. The present study examined whether 1,25(OH)2D3 has an effect on autophagy in peritoneal mesothelial cells. The protein level of Beclin, anti‑ubiquitin‑binding protein p62 (p62), microtubule‑associated proteins 1A/1B light chain 3B (LC3-II), mechanistic target of rapamycin (mTOR) and phosphorylated mTOR were evaluated by western blot analysis. Autophagosomes were detected under transmission electron microscopy. It was revealed that exposure to HG inhibited autophagy in peritoneal mesothelial cells. However, 1,25(OH)2D3 alleviated autophagy inhibition induced by HG in human peritoneal mesothelial cells, which activated expression of autophagy‑associated genes encoding Beclin‑1 and LC3-II downregulated the expression of p62 via mTOR signaling pathway. In a mouse model of HG‑treated peritoneal mesothelium, autophagy inhibition was observed in peritoneum, 1,25(OH)2D3 attenuated HG‑induced autophagy inhibition in peritoneal mesothelium via the mTOR signaling pathway. These findings suggested that 1,25(OH)2D3 may be a potential therapy for peritoneal injury.
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Copy and paste a formatted citation
Spandidos Publications style
Yang L, Fan Y, Zhang X, Liu J and Ma J: Effect of 1,25(OH)2D3 on high glucose‑induced autophagy inhibition in peritoneum. Mol Med Rep 16: 7080-7085, 2017.
APA
Yang, L., Fan, Y., Zhang, X., Liu, J., & Ma, J. (2017). Effect of 1,25(OH)2D3 on high glucose‑induced autophagy inhibition in peritoneum. Molecular Medicine Reports, 16, 7080-7085. https://doi.org/10.3892/mmr.2017.7408
MLA
Yang, L., Fan, Y., Zhang, X., Liu, J., Ma, J."Effect of 1,25(OH)2D3 on high glucose‑induced autophagy inhibition in peritoneum". Molecular Medicine Reports 16.5 (2017): 7080-7085.
Chicago
Yang, L., Fan, Y., Zhang, X., Liu, J., Ma, J."Effect of 1,25(OH)2D3 on high glucose‑induced autophagy inhibition in peritoneum". Molecular Medicine Reports 16, no. 5 (2017): 7080-7085. https://doi.org/10.3892/mmr.2017.7408
Copy and paste a formatted citation
x
Spandidos Publications style
Yang L, Fan Y, Zhang X, Liu J and Ma J: Effect of 1,25(OH)2D3 on high glucose‑induced autophagy inhibition in peritoneum. Mol Med Rep 16: 7080-7085, 2017.
APA
Yang, L., Fan, Y., Zhang, X., Liu, J., & Ma, J. (2017). Effect of 1,25(OH)2D3 on high glucose‑induced autophagy inhibition in peritoneum. Molecular Medicine Reports, 16, 7080-7085. https://doi.org/10.3892/mmr.2017.7408
MLA
Yang, L., Fan, Y., Zhang, X., Liu, J., Ma, J."Effect of 1,25(OH)2D3 on high glucose‑induced autophagy inhibition in peritoneum". Molecular Medicine Reports 16.5 (2017): 7080-7085.
Chicago
Yang, L., Fan, Y., Zhang, X., Liu, J., Ma, J."Effect of 1,25(OH)2D3 on high glucose‑induced autophagy inhibition in peritoneum". Molecular Medicine Reports 16, no. 5 (2017): 7080-7085. https://doi.org/10.3892/mmr.2017.7408
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