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Article

Adiponectin receptor agonist AdipoRon suppresses adipogenesis in C3H10T1/2 cells through the adenosine monophosphate‑activated protein kinase signaling pathway

  • Authors:
    • Shu‑Juan Wang
    • Wen‑Yi Lu
    • Kai‑Yan Liu
  • View Affiliations / Copyright

    Affiliations: Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Peking University People's Hospital and Institute of Hematology, Beijing 100044, P.R. China
  • Pages: 7163-7169
    |
    Published online on: September 8, 2017
       https://doi.org/10.3892/mmr.2017.7450
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Abstract

The aim of the present study was to investigate the effects of AdipoRon, an adiponectin receptor agonist, on adipogenesis in C3H10T1/2 cells and to explore the underlying mechanisms. C3H10T1/2 cells were treated with increasing doses of AdipoRon for 8 days, and Oil Red O staining was used to assess lipid accumulation. The protein and mRNA expression levels of adipogenic transcription factors and adipocyte‑specific genes were examined by western blotting and reverse transcription quantitative polymerase chain reaction, respectively. AdipoRon treatment inhibited lipid accumulation in C3H10T1/2 cells in a dose‑dependent manner and significantly suppressed the expression of adipogenic transcription factors, including peroxisome proliferator‑activated receptor γ, CAAT/enhancer binding protein (C/EBP)‑β and C/EBPα. In addition, cells treated with AdipoRon exhibited a significant decrease in the expression of adipocyte‑specific genes, including fatty acid binding protein 4, fatty acid synthase, leptin, adiponectin, and stearoyl‑CoA desaturase‑1. Notably, AdipoRon significantly increased the phosphorylation of adenosine monophosphate‑activated protein kinase (AMPK) and acetyl‑CoA carboxylase (ACC). The results indicated that AdipoRon exerted an inhibitory effect on adipogenesis in C3H10T1/2 cells by downregulating the expression of adipogenic transcription factors and adipocyte‑specific genes and by promoting the phosphorylation of AMPK and ACC, which suggested that AdipoRon may be a potential drug to prevent and treat diseases caused by abnormal adipogenesis, such as obesity.
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1 

Despres JP and Lemieux I: Abdominal obesity and metabolic syndrome. Nature. 444:881–887. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Ko SC, Lee M, Lee JH, Lee SH, Lim Y and Jeon YJ: Dieckol, a phlorotannin isolated from a brown seaweed, Ecklonia cava, inhibits adipogenesis through AMP-activated protein kinase (AMPK) activation in 3T3-L1 preadipocytes. Environ Toxicol Pharmacol. 36:1253–1260. 2013. View Article : Google Scholar : PubMed/NCBI

3 

White UA and Stephens JM: Transcriptional factors that promote formation of white adipose tissue. Mol Cell Endocrinol. 318:10–14. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Rosen ED and Spiegelman BM: PPARgamma: A nuclear regulator of metabolism, differentiation, and cell growth. J Biol Chem. 276:37731–37734. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Wu Z, Rosen ED, Brun R, Hauser S, Adelmant G, Troy AE, McKeon C, Darlington GJ and Spiegelman BM: Cross-regulation of C/EBP alpha and PPAR gamma controls the transcriptional pathway of adipogenesis and insulin sensitivity. Mol Cell. 3:151–158. 1999. View Article : Google Scholar : PubMed/NCBI

6 

Lee JH, Kim T, Lee JJ, Lee KJ, Kim HK, Yun B, Jeon J, Kim SK and Ma JY: The herbal medicine KBH-1 inhibits fat accumulation in 3T3-L1 adipocytes and reduces high fat diet-induced obesity through regulation of the AMPK pathway. PLoS One. 10:e01420412015. View Article : Google Scholar : PubMed/NCBI

7 

Lin JT, Chen HM, Chiu CH and Liang YJ: AMP-activated protein kinase activators in diabetic ulcers: From animal studies to phase II drugs under investigation. Expert Opin Investig Drugs. 23:1253–1265. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Kim SK and Kong CS: Anti-adipogenic effect of dioxinodehydroeckol via AMPK activation in 3T3-L1 adipocytes. Chem Biol Interact. 186:24–29. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Ceddia RB: The role of AMP-activated protein kinase in regulating white adipose tissue metabolism. Mol Cell Endocrinol. 366:194–203. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Hotta K, Funahashi T, Arita Y, Takahashi M, Matsuda M, Okamoto Y, Iwahashi H, Kuriyama H, Ouchi N, Maeda K, et al: Plasma concentrations of a novel, adipose-specific protein, adiponectin, in type 2 diabetic patients. Arterioscler Thromb Vasc Biol. 20:1595–1599. 2000. View Article : Google Scholar : PubMed/NCBI

11 

Yamauchi T, Kamon J, Waki H, Terauchi Y, Kubota N, Hara K, Mori Y, Ide T, Murakami K, Tsuboyama-Kasaoka N, et al: The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity. Nat Med. 7:941–946. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Okada-Iwabu M, Yamauchi T, Iwabu M, Honma T, Hamagami K, Matsuda K, Yamaguchi M, Tanabe H, Kimura-Someya T, Shirouzu M, et al: A small-molecule AdipoR agonist for type 2 diabetes and short life in obesity. Nature. 503:493–499. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Holland WL and Scherer PE: Cell Biology. Ronning after the adiponectin receptors. Science. 342:1460–1461. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Lazra Y, Falach A, Frenkel L, Rozenberg K, Sampson S and Rosenzweig T: Autocrine/paracrine function of globular adiponectin: Inhibition of lipid metabolism and inflammatory response in 3T3-L1 adipocytes. J Cell Biochem. 116:754–766. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Poudel B, Nepali S, Xin M, Ki HH, Kim YH, Kim DK and Lee YM: Flavonoids from Triticum aestivum inhibit adipogenesis in 3T3-L1 cells by upregulating the insig pathway. Mol Med Rep. 12:3139–3145. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Cantó C, Gerhart-Hines Z, Feige JN, Lagouge M, Noriega L, Milne JC, Elliott PJ, Puigserver P and Auwerx J: AMPK regulates energy expenditure by modulating NAD+ metabolism and SIRT1 activity. Nature. 458:1056–1060. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Liou CJ, Lai XY, Chen YL, Wang CL, Wei CH and Huang WC: Ginkgolide C suppresses adipogenesis in 3T3-L1 adipocytes via the AMPK signaling pathway. Evid Based Complement Alternat Med. 2015:2986352015. View Article : Google Scholar : PubMed/NCBI

19 

Lee YK, Lee WS, Hwang JT, Kwon DY, Surh YJ and Park OJ: Curcumin exerts antidifferentiation effect through AMPKalpha-PPAR-gamma in 3T3-L1 adipocytes and antiproliferatory effect through AMPKalpha-COX-2 in cancer cells. J Agric Food Chem. 57:305–310. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Ahn J, Lee H, Kim S, Park J and Ha T: The anti-obesity effect of quercetin is mediated by the AMPK and MAPK signaling pathways. Biochem Biophys Res Commun. 373:545–549. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Chen S, Li Z, Li W, Shan Z and Zhu W: Resveratrol inhibits cell differentiation in 3T3-L1 adipocytes via activation of AMPK. Can J Physiol Pharmacol. 89:793–799. 2011.PubMed/NCBI

22 

Daval M, Foufelle F and Ferré P: Functions of AMP-activated protein kinase in adipose tissue. J Physiol. 574:55–62. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Giri S, Rattan R, Haq E, Khan M, Yasmin R, Won JS, Key L, Singh AK and Singh I: AICAR inhibits adipocyte differentiation in 3T3L1 and restores metabolic alterations in diet-induced obesity mice model. Nutr Metab (Lond). 3:312006. View Article : Google Scholar : PubMed/NCBI

24 

Habinowski SA and Witters LA: The effects of AICAR on adipocyte differentiation of 3T3-L1 cells. Biochem Biophys Res Commun. 286:852–856. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Lee H, Kang R, Bae S and Yoon Y: AICAR, an activator of AMPK, inhibits adipogenesis via the WNT/β-catenin pathway in 3T3-L1 adipocytes. Int J Mol Med. 28:65–71. 2011.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang SJ, Lu WY and Liu KY: Adiponectin receptor agonist AdipoRon suppresses adipogenesis in C3H10T1/2 cells through the adenosine monophosphate‑activated protein kinase signaling pathway. Mol Med Rep 16: 7163-7169, 2017.
APA
Wang, S., Lu, W., & Liu, K. (2017). Adiponectin receptor agonist AdipoRon suppresses adipogenesis in C3H10T1/2 cells through the adenosine monophosphate‑activated protein kinase signaling pathway. Molecular Medicine Reports, 16, 7163-7169. https://doi.org/10.3892/mmr.2017.7450
MLA
Wang, S., Lu, W., Liu, K."Adiponectin receptor agonist AdipoRon suppresses adipogenesis in C3H10T1/2 cells through the adenosine monophosphate‑activated protein kinase signaling pathway". Molecular Medicine Reports 16.5 (2017): 7163-7169.
Chicago
Wang, S., Lu, W., Liu, K."Adiponectin receptor agonist AdipoRon suppresses adipogenesis in C3H10T1/2 cells through the adenosine monophosphate‑activated protein kinase signaling pathway". Molecular Medicine Reports 16, no. 5 (2017): 7163-7169. https://doi.org/10.3892/mmr.2017.7450
Copy and paste a formatted citation
x
Spandidos Publications style
Wang SJ, Lu WY and Liu KY: Adiponectin receptor agonist AdipoRon suppresses adipogenesis in C3H10T1/2 cells through the adenosine monophosphate‑activated protein kinase signaling pathway. Mol Med Rep 16: 7163-7169, 2017.
APA
Wang, S., Lu, W., & Liu, K. (2017). Adiponectin receptor agonist AdipoRon suppresses adipogenesis in C3H10T1/2 cells through the adenosine monophosphate‑activated protein kinase signaling pathway. Molecular Medicine Reports, 16, 7163-7169. https://doi.org/10.3892/mmr.2017.7450
MLA
Wang, S., Lu, W., Liu, K."Adiponectin receptor agonist AdipoRon suppresses adipogenesis in C3H10T1/2 cells through the adenosine monophosphate‑activated protein kinase signaling pathway". Molecular Medicine Reports 16.5 (2017): 7163-7169.
Chicago
Wang, S., Lu, W., Liu, K."Adiponectin receptor agonist AdipoRon suppresses adipogenesis in C3H10T1/2 cells through the adenosine monophosphate‑activated protein kinase signaling pathway". Molecular Medicine Reports 16, no. 5 (2017): 7163-7169. https://doi.org/10.3892/mmr.2017.7450
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