Vulnerability study of myelinated and unmyelinated nerve fibers in acute ocular hypertension in rabbit

Shen,Jiying; Yang,Qiang; Yu,Daoyi; Wu,Jihong; Zhu,Yuanfang; Guo,Wenyi

doi:10.3892/mmr.2017.7474

November-2017 Volume 16 Issue 5

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November-2017 Volume 16 Issue 5

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Vulnerability study of myelinated and unmyelinated nerve fibers in acute ocular hypertension in rabbit

Authors:
- Jiying Shen
- Qiang Yang
- Daoyi Yu
- Jihong Wu
- Yuanfang Zhu
- Wenyi Guo
View Affiliations / Copyright

Affiliations: Department of Ophthalmology, Shanghai Peace Eye Hospital, Shanghai 200437, P.R. China, Department of Ophthalmology, Eye & ENT Hospital of Fudan University, Shanghai 200031, P.R. China, Centre for Ophthalmology & Visual Science, The University of Western Australia, Perth, WA 6009, Australia

Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 6794-6802
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Published online on: September 12, 2017

https://doi.org/10.3892/mmr.2017.7474
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Abstract

In the current study, it was aimed to evaluate the changes in myelinated and unmyelinated nerve fibers in retinal ischemia‑reperfusion injuries caused by acute ocular hypertension and to determine the sequence of these changes. Adult healthy New Zealand white rabbits were randomized to the hemodynamic group [n=12; used to determine the optimal intraocular pressure (IOP) for the subsequent experiments] and the hypertension group (n=6; 70‑mmHg hypertension induced in one eye). IOP was adjusted using a cannula and saline. Doppler ultrasound was used to measure the velocity of the optic artery under different intraocular pressures. Immunohistochemistry for myelin basic protein (MBP) was performed. Apoptosis of retinal cells was detected by terminal deoxynucleotidyl transferase biotin‑dUTP nick end labeling (TUNEL) assay. Electron microscopy was used to investigate the changes in myelinated and unmyelinated nerve fibers. IOP of the hypertension eyes was maintained at 70.2±1.0 mmHg, while IOP of control eyes was 7‑14 mmHg. Doppler ultrasound demonstrated an obvious decline of peak systolic velocity and an increase of resistance index of retinal bloodstream under a 70‑mmHg IOP. MBP immunohistochemistry and electron microscopy demonstrated obvious injuries to the myelin fibers. TUNEL indicated a significantly higher apoptosis rate in the hypertension eyes compared with control eyes. The apoptosis rate of retinal ganglion cells and bipolar cells in unmyelinated regions was higher than in myelinated regions. In conclusion, an IOP of 70 mmHg led to incomplete retinal ischemia but was the threshold for retinal ischemia, leading to obvious injuries to the myelin fibers.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Vulnerability study of myelinated and unmyelinated nerve fibers in acute ocular hypertension in rabbit

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