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Article

MicroRNA‑146a promotes the proliferation of rat vascular smooth muscle cells by downregulating p53 signaling

  • Authors:
    • Yu Luo
    • Wei Xiong
    • Shaohong Dong
    • Feng Liu
    • Huadong Liu
    • Jianghua Li
  • View Affiliations / Copyright

    Affiliations: Department of Gerontology, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China, Department of Cardiology, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China
  • Pages: 6940-6945
    |
    Published online on: September 12, 2017
       https://doi.org/10.3892/mmr.2017.7477
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Abstract

The present study aimed to detect and verify gene expression profile differences for microRNA (miR)‑146a and its role in the proliferation of vascular smooth muscle cells (VSMCs). Artificially synthesized miR‑146a mimics, miR‑146 inhibitor, scramble‑miRNA or PBS was transfected into cultured primary rat VSMCs in vitro. Reverse transcription‑quantitative polymerase chain reaction confirmed that the miR‑146a expression level was significantly decreased in VSMCs treated with miR‑146a inhibitor (P<0.01). Cell Counting Kit‑8 was used to determine the proliferation ability, which demonstrated that proliferation was significantly decreased in VSMCs treated with miR‑146a inhibitor (P<0.01). Microarray expression profiling analysis revealed that the p53 signal pathway was upregulated in VSMCs treated with the miR‑146a inhibitor. Compared with untransfected VSMCs, the mRNA and protein expression levels of caspase‑3 and phosphatase and tensin homolog (PTEN) in p53 signal transduction pathway did not exhibit a significant difference (P>0.05); however, the mRNA and protein expression levels of p53 were significantly decreased in cells transfected with miR‑146a mimics and increased in miR‑146a inhibitor transfected cells (both P<0.01). The mRNA and protein expression levels of cyclin D1 significantly increased in miR‑146a mimics transfected cells and decreased in cells transfected with the miR‑146a inhibitor (both P<0.05). The present data indicated that miR‑146a may promote the proliferation of rat VSMCs by downregulating p53 and upregulating cyclin D1 expression.
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1 

Pasterkamp G, de Kleijn DP and Borst C: Arterial remodeling in atherosclerosis, restenosis and after alteration of blood flow: Potential mechanisms and clinical implications. Cardiovasc Res. 45:843–852. 2000. View Article : Google Scholar : PubMed/NCBI

2 

Davis-Dusenbery BN, Wu C and Hata A: Micromanaging vascular smooth muscle cell differentiation and phenotypic modulation. Arterioscler Thromb Vasc Biol. 31:2370–2377. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Esteller M: Non-coding RNAs in human disease. Nat Rev Genet. 12:861–874. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Small EM and Olson EN: Pervasive roles of microRNAs in cardiovascular biology. Nature. 469:336–342. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Fazi F and Nervi C: MicroRNA: Basic mechanisms and transcriptional regulatory networks for cell fate determination. Cardiovasc Res. 79:553–561. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Chan EK, Ceribelli A and Satoh M: MicroRNA-146a in autoimmunity and innate immune responses. Ann Rheum Dis. 72 Suppl 2:ii90–ii95. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Guo M, Mao X, Ji Q, Lang M, Li S, Peng Y, Zhou W, Xiong B and Zeng Q: MiR-146a in PBMCs modulates Th1 function in patients with acute coronary syndrome. Immunol Cell Biol. 88:555–564. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Ji R, Cheng Y, Yue J, Yang J, Liu X, Chen H, Dean DB and Zhang C: MicroRNA expression signature and antisense-mediated depletion reveal an essential role of microRNA in vascular neointimal lesion formation. Circ Res. 100:1579–1588. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Li K, Ching D, Luk FS and Raffai RL: Apolipoprotein E enhances microRNA-146a in monocytes and macrophages to suppress nuclear factor-κB-driven inflammation and atherosclerosis. Circ Res. 117:e1–e11. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Dong S, Xiong W, Yuan J, Li J, Liu J and Xu X: MiRNA-146a regulates the maturation and differentiation of vascular smooth muscle cells by targeting NF-κB expression. Mol Med Rep. 8:407–412. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Creemers EE, Tijsen AJ and Pinto YM: Circulating microRNAs: Novel biomarkers and extracellular communicators in cardiovascular disease? Circ Res. 110:483–495. 2012. View Article : Google Scholar : PubMed/NCBI

12 

van Rooij E and Olson EN: MicroRNA therapeutics for cardiovascular disease: Opportunities and obstacles. Nat Rev Drug Discov. 11:860–872. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Li L, Chen XP and Li YJ: MicroRNA-146a and human disease. Scand J Immunol. 71:227–231. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Taganov KD, Boldin MP, Chang KJ and Baltimore D: NF-kappaB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses. Proc Natl Acad Sci USA. 103:12481–12486. 2006; View Article : Google Scholar : PubMed/NCBI

15 

Bhaumik D, Scott GK, Schokrpur S, Patil CK, Campisi J and Benz CC: Expression of microRNA-146 suppresses NF-kappaB activity with reduction of metastatic potential in breast cancer cells. Oncogene. 27:5643–5647. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Pauley KM, Satoh M, Chan AL, Bubb MR, Reeves WH and Chan EK: Upregulated miR-146a expression in peripheral blood mononuclear cells from rheumatoid arthritis patients. Arthritis Res Ther. 10:R1012008. View Article : Google Scholar : PubMed/NCBI

17 

Pacifico F, Crescenzi E, Mellone S, Iannetti A, Porrino N, Liguoro D, Moscato F, Grieco M, Formisano S and Leonardi A: Nuclear factor-{kappa}B contributes to anaplastic thyroid carcinomas through up-regulation of miR-146a. J Clin Endocrinol Metab. 95:1421–1430. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Wang JF, Yu ML, Yu G, Bian JJ, Deng XM, Wan XJ and Zhu KM: Serum miR-146a and miR-223 as potential new biomarkers for sepsis. Biochem Biophys Res Commun. 394:184–188. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Curtiss LK and Boisvert WA: Apolipoprotein E and atherosclerosis. Curr Opin Lipidol. 11:243–251. 2000. View Article : Google Scholar : PubMed/NCBI

20 

Liu X, Dong Y, Chen S, Zhang G, Zhang M, Gong Y and Li X: Circulating MicroRNA-146a and microRNA-21 predict left ventricular remodeling after ST-elevation myocardial infarction. Cardiology. 132:233–241. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Wassmann S1, Wassmann K, Jung A, Velten M, Knuefermann P, Petoumenos V, Becher U, Werner C, Mueller C and Nickenig G: Induction of p53 by GKLF is essential for inhibition of proliferation of vascular smooth muscle cells. J Mol Cell Cardiol. 43:301–307. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Guevara NV, Kim HS, Antonova EI and Chan L: The absence of p53 accelerates atherosclerosis by increasing cell proliferation in vivo. Nat Med. 5:335–339. 1999. View Article : Google Scholar : PubMed/NCBI

23 

Ghose J, Sinha M, Das E, Jana NR and Bhattacharyya NP: Regulation of miR-146a by RelA/NFκB and p53 in STHdh(Q111)/Hdh(Q111) cells, a cell model of Huntington's disease. PLoS One. 6:e238372011. View Article : Google Scholar : PubMed/NCBI

24 

Pestell RG: New roles of cyclin D1. Am J Pathol. 183:3–9. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Kim MH, Ham O, Lee SY, Choi E, Lee CY, Park JH, Lee J, Seo HH, Seung M, Choi E, et al: MicroRNA-365 inhibits the proliferation of vascular smooth muscle cells by targeting cyclin D1. J Cell Biochem. 115:1752–1761. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Luo Y, Xiong W, Dong S, Liu F, Liu H and Li J: MicroRNA‑146a promotes the proliferation of rat vascular smooth muscle cells by downregulating p53 signaling. Mol Med Rep 16: 6940-6945, 2017.
APA
Luo, Y., Xiong, W., Dong, S., Liu, F., Liu, H., & Li, J. (2017). MicroRNA‑146a promotes the proliferation of rat vascular smooth muscle cells by downregulating p53 signaling. Molecular Medicine Reports, 16, 6940-6945. https://doi.org/10.3892/mmr.2017.7477
MLA
Luo, Y., Xiong, W., Dong, S., Liu, F., Liu, H., Li, J."MicroRNA‑146a promotes the proliferation of rat vascular smooth muscle cells by downregulating p53 signaling". Molecular Medicine Reports 16.5 (2017): 6940-6945.
Chicago
Luo, Y., Xiong, W., Dong, S., Liu, F., Liu, H., Li, J."MicroRNA‑146a promotes the proliferation of rat vascular smooth muscle cells by downregulating p53 signaling". Molecular Medicine Reports 16, no. 5 (2017): 6940-6945. https://doi.org/10.3892/mmr.2017.7477
Copy and paste a formatted citation
x
Spandidos Publications style
Luo Y, Xiong W, Dong S, Liu F, Liu H and Li J: MicroRNA‑146a promotes the proliferation of rat vascular smooth muscle cells by downregulating p53 signaling. Mol Med Rep 16: 6940-6945, 2017.
APA
Luo, Y., Xiong, W., Dong, S., Liu, F., Liu, H., & Li, J. (2017). MicroRNA‑146a promotes the proliferation of rat vascular smooth muscle cells by downregulating p53 signaling. Molecular Medicine Reports, 16, 6940-6945. https://doi.org/10.3892/mmr.2017.7477
MLA
Luo, Y., Xiong, W., Dong, S., Liu, F., Liu, H., Li, J."MicroRNA‑146a promotes the proliferation of rat vascular smooth muscle cells by downregulating p53 signaling". Molecular Medicine Reports 16.5 (2017): 6940-6945.
Chicago
Luo, Y., Xiong, W., Dong, S., Liu, F., Liu, H., Li, J."MicroRNA‑146a promotes the proliferation of rat vascular smooth muscle cells by downregulating p53 signaling". Molecular Medicine Reports 16, no. 5 (2017): 6940-6945. https://doi.org/10.3892/mmr.2017.7477
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