MicroRNA‑214 targets Wnt3a to suppress liver cancer cell proliferation

Yang,Yang; Zhao,Zhenghao; Hou,Ni; Li,Yulong; Wang,Xiaofei; Wu,Fei; Sun,Ruifang; Han,Jia; Sun,Hongfei; Song,Tusheng; Huang,Chen; Shao,Yuan

doi:10.3892/mmr.2017.7483

MicroRNA‑214 targets Wnt3a to suppress liver cancer cell proliferation

Authors:
- Yang Yang
- Zhenghao Zhao
- Ni Hou
- Yulong Li
- Xiaofei Wang
- Fei Wu
- Ruifang Sun
- Jia Han
- Hongfei Sun
- Tusheng Song
- Chen Huang
- Yuan Shao
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Affiliations: Department of Health Toxicology and Hygiene Inspection, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Gastroenterology, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, P.R. China, Department of Pathology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Otorhinolaryngology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
Published online on: September 13, 2017 https://doi.org/10.3892/mmr.2017.7483
Pages: 6920-6927

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Abstract

MicroRNAs (miRNAs/miRs) are crucial molecules that act as tumor suppressor genes or oncogenes in human cancer progression. The dysregulation of miRNA expression has been detected in liver cancer. The present study aimed to explore the molecular mechanisms by which miR‑214 affects liver cancer cell proliferation. Reverse transcription‑quantitative polymerase chain reaction was used to determine the expression of miR‑214 in liver cancer cell lines and hepatocellular carcinoma (HCC) tissues. A luciferase reporter assay was performed to determine whether Wnt3a is a target gene of miR‑214. Cell Counting kit‑8 and cell cycle analysis were used to explore the effects of miR‑214 on liver cancer cell proliferation. Immunohistochemistry was used to detect protein expression levels. Wnt3a knockdown was used to determine the function of Wnt3a in liver cancer cell proliferation. The results demonstrated that the expression levels of human miR‑214 were reduced in HCC tissues and liver cancer cell lines compared with in control tissues and cells. Overexpression of miR‑214 and Wnt3a silencing each inhibited liver cancer cell growth. Conversely, inhibition of miR‑214 promoted liver cancer cell growth. The present study indicated that miR‑214 acts as a tumor suppressor and may be considered a promising therapeutic target for the treatment of liver cancer.

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