Ferruginol exhibits anticancer effects in OVCAR‑3 human ovary cancer cells by inducing apoptosis, inhibition of cancer cell migration and G2/M phase cell cycle arrest Retraction in /10.3892/mmr.2021.11868

Xiong,Wen‑Dong; Gong,Jian; Xing,Chao

doi:10.3892/mmr.2017.7484

November-2017 Volume 16 Issue 5

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November-2017 Volume 16 Issue 5

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Article

Ferruginol exhibits anticancer effects in OVCAR‑3 human ovary cancer cells by inducing apoptosis, inhibition of cancer cell migration and G2/M phase cell cycle arrest

Retraction in: /10.3892/mmr.2021.11868

Authors:
- Wen‑Dong Xiong
- Jian Gong
- Chao Xing
View Affiliations / Copyright

Affiliations: Department of Obstetrics and Gynecology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China, Department of Clinical Laboratory, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China
Pages: 7013-7017
|
Published online on: September 13, 2017

https://doi.org/10.3892/mmr.2017.7484
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Abstract

The primary aim of the current study was to investigate the antitumor effects of ferruginol in OVCAR‑3 human ovary cancer cells. The effects of ferruginol on cell apoptosis, cell migration and cell cycle phase distribution were also evaluated. Cell cytotoxicity induced by ferruginol was determined by an MTT assay, while fluorescence microscopy and transmission electron microscopy (TEM) were performed to investigate apoptotic effects. Flow cytometry was employed to determine the effects of ferruginol on the cell cycle and an in vitro wound healing assay was performed to investigate effects on cancer cell migration. The results indicated that ferruginol inhibited the growth rate of OVACR‑3 cells in a dose‑ and time‑dependent manner. When cells were treated with 20, 80 and 300 µM ferruginol, cells began to exhibit yellow fluorescence, which indicated the onset of apoptosis. TEM results demonstrated that untreated control cells exhibited intact nuclei and nucleolus. However, on treating cells with various doses of ferruginol, chromatin condensation occurred and disappearance of the nuclear envelope and formation of apoptotic bodies were also observed. The percentage of migrated cells, determined by the wound healing assay, decreased from 98.7% in control to 68.2% and 45.3 in 80 and 300 µM ferruginol‑treated cells, respectively. Flow cytometry results demonstrated that ferruginol induced G2/M cell cycle arrest in OVCAR‑3 cells. In conclusion, ferruginol may exhibit anticancer effects in OVCAR‑3 human ovary cancer cells by inducing apoptosis, inhibiting cancer cell migration and inducing G2/M cell and may therefore prove beneficial in the treatment and management of ovarian cancer.

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1	Jemal A, Siegel R, Xu J and Ward E: Cancer statistics, 2010. CA Cancer J Clin. 60:277–300. 2010. View Article : Google Scholar : PubMed/NCBI
2	Tingulstad S, Skjeldestad FE, Halvorsen TB and Hagen B: Survival and prognostic factors in patients with ovarian cancer. Obstet Gynecol. 101:885–891. 2003. View Article : Google Scholar : PubMed/NCBI
3	Kurman RJ and Shih IeM: The origin and pathogenesis of epithelial ovarian cancer: A proposed unifying theory. Am J Surg Pathol. 34:433–443. 2010. View Article : Google Scholar : PubMed/NCBI
4	Agarwal R and Kaye SB: Ovarian cancer: Strategies for overcoming resistance to chemotherapy. Nat Rev Cancer. 3:502–516. 2003. View Article : Google Scholar : PubMed/NCBI
5	Ozols RF, Bookman MA, Connolly DC, Daly MB, Godwin AK, Schilder RJ, Xu X and Hamilton TC: Focus on epithelial ovarian cancer. Cancer Cell. 5:19–24. 2004. View Article : Google Scholar : PubMed/NCBI
6	Hille S, Rein DT, Riffelmann M, Neumann R, Sartorius J, Pfützner A, Kurbacher CM, Schöndorf T and Breidenbach M: Anticancer drugs induce mdr1 gene expression in recurrent ovarian cancer. Anticancer Drugs. 17:1041–1044. 2006. View Article : Google Scholar : PubMed/NCBI
7	Jackson RC: The problem of the quiescent cancer cell. Adv Enzyme Regul. 29:27–46. 1989. View Article : Google Scholar : PubMed/NCBI
8	Mellor HR, Ferguson DJ and Callaghan R: A model of quiescent tumour microregions for evaluating multicellular resistance to chemotherapeutic drugs. Br J Cancer. 93:302–309. 2005. View Article : Google Scholar : PubMed/NCBI
9	Liang CC, Park AY and Guan JL: In vitro scratch assay: A convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc. 2:329–333. 2007. View Article : Google Scholar : PubMed/NCBI
10	Green DR: Means to an End: Apoptosis and Other Cell Death Mechanisms. Cold Spring Harbor Laboratory Press; Long Island, NY: 2011
11	Jose A. Karam and Jer-Tsong Hsieh: Anti-cancer strategy of transitional cell carcinoma of bladder basedon induction of different types of programmed cell deaths: Apoptosis in Carcinogenesis and Chemotherapy. Springer; Netherlands: pp. 25–50. 2009
12	D'Amico AV and McKenna WG: Apoptosis and a re-investigation of the biologic basis forcancer therapy. Radiother Oncol. 33:3–10. 1994. View Article : Google Scholar : PubMed/NCBI
13	Makin G and Dive C: Apoptosis and cancer chemotherapy. Trends Cell Biol. 11 Suppl:S22–S26. 2001. View Article : Google Scholar : PubMed/NCBI
14	Fulda S and Debatin KM: Targeting apoptosis pathways in cancer therapy. Curr Cancer Drug Targets. 4:569–576. 2004. View Article : Google Scholar : PubMed/NCBI
15	Koehn FE and Carter GT: The evolving role of natural products in drug discovery. Nat Rev Drug Discov. 4:206–220. 2005. View Article : Google Scholar : PubMed/NCBI
16	Newman DJ and Cragg GM: Natural products as sources of new drugs over the 30 years from 1981 to 2010. J NatProd. 75:311–335. 2012.
17	Chin YW, Yoon KD and Kim J: Cytotoxic anticancer candidates from terrestrial plants. Anticancer Agents Med Chem. 9:913–942. 2009. View Article : Google Scholar : PubMed/NCBI
18	Son KH, Oh HM, Choi SK, Han DC and Kwon BM: Anti-tumor abietane diterpenes from the cones of Sequoia sempervirens. Bioorg Med Chem Lett. 15:2019–2021. 2005. View Article : Google Scholar : PubMed/NCBI
19	Ho ST, Tung YT, Kuo YH, Lin CC and Wu JH: Ferruginol inhibits non-small cell lung cancer growth by inducing caspase-associated apoptosis. Integr Cancer Ther. 14:86–97. 2015. View Article : Google Scholar : PubMed/NCBI
20	Areche C, Theoduloz C, Yáñez T, Souza-Brito AR, Barbastefano V, de Paula D, Ferreira AL, Schmeda-Hirschmann G and Rodríguez JA: Gastroprotective activity of ferruginol in mice and rats: Effects on gastric secretion, endogenous prostaglandins and non-protein sulfhydryls. J Pharm Pharmacol. 60:245–251. 2008. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Ferruginol exhibits anticancer effects in OVCAR‑3 human ovary cancer cells by inducing apoptosis, inhibition of cancer cell migration and G2/M phase cell cycle arrest

Retraction in: /10.3892/mmr.2021.11868

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