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UPLC‑QTOFMS‑based metabolomic analysis of the serum of hypoxic preconditioning mice

  • Authors:
    • Jie Liu
    • Gang Zhan
    • Dewei Chen
    • Jian Chen
    • Zhi‑Bin Yuan
    • Er‑Long Zhang
    • Yi‑Xing Gao
    • Gang Xu
    • Bing‑Da Sun
    • Wenting Liao
    • Yu‑Qi Gao
  • View Affiliations / Copyright

    Affiliations: Institute of Medicine and Hygienic Equipment for High Altitude Region, College of High Altitude Military Medicine, Third Military Medical University, Chongqing 400038, P.R. China, Key Laboratory of High Altitude Medicine, Ministry of Education, College of High Altitude Military Medicine, Third Military Medical University, Chongqing 400038, P.R. China, Department of Pharmaceutical Analysis, School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 210009, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6828-6836
    |
    Published online on: September 13, 2017
       https://doi.org/10.3892/mmr.2017.7493
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Abstract

Hypoxic preconditioning (HPC) is well‑known to exert a protective effect against hypoxic injury; however, the underlying molecular mechanism remains unclear. The present study utilized a serum metabolomics approach to detect the alterations associated with HPC. In the present study, an animal model of HPC was established by exposing adult BALB/c mice to acute repetitive hypoxia four times. The serum samples were collected by orbital blood sampling. Metabolite profiling was performed using ultra‑performance liquid chromatography‑quadrupole time‑of‑flight mass spectrometry (UPLC‑QTOFMS), in conjunction with univariate and multivariate statistical analyses. The results of the present study confirmed that the HPC mouse model was established and refined, suggesting significant differences between the control and HPC groups at the molecular levels. HPC caused significant metabolic alterations, as represented by the significant upregulation of valine, methionine, tyrosine, isoleucine, phenylalanine, lysophosphatidylcholine (LysoPC; 16:1), LysoPC (22:6), linoelaidylcarnitine, palmitoylcarnitine, octadecenoylcarnitine, taurine, arachidonic acid, linoleic acid, oleic acid and palmitic acid, and the downregulation of acetylcarnitine, malate, citrate and succinate. Using MetaboAnalyst 3.0, a number of key metabolic pathways were observed to be acutely perturbed, including valine, leucine and isoleucine biosynthesis, in addition to taurine, hypotaurine, phenylalanine, linoleic acid and arachidonic acid metabolism. The results of the present study provided novel insights into the mechanisms involved in the acclimatization of organisms to hypoxia, and demonstrated the protective mechanism of HPC.
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Copy and paste a formatted citation
Spandidos Publications style
Liu J, Zhan G, Chen D, Chen J, Yuan ZB, Zhang EL, Gao YX, Xu G, Sun BD, Liao W, Liao W, et al: UPLC‑QTOFMS‑based metabolomic analysis of the serum of hypoxic preconditioning mice. Mol Med Rep 16: 6828-6836, 2017.
APA
Liu, J., Zhan, G., Chen, D., Chen, J., Yuan, Z., Zhang, E. ... Gao, Y. (2017). UPLC‑QTOFMS‑based metabolomic analysis of the serum of hypoxic preconditioning mice. Molecular Medicine Reports, 16, 6828-6836. https://doi.org/10.3892/mmr.2017.7493
MLA
Liu, J., Zhan, G., Chen, D., Chen, J., Yuan, Z., Zhang, E., Gao, Y., Xu, G., Sun, B., Liao, W., Gao, Y."UPLC‑QTOFMS‑based metabolomic analysis of the serum of hypoxic preconditioning mice". Molecular Medicine Reports 16.5 (2017): 6828-6836.
Chicago
Liu, J., Zhan, G., Chen, D., Chen, J., Yuan, Z., Zhang, E., Gao, Y., Xu, G., Sun, B., Liao, W., Gao, Y."UPLC‑QTOFMS‑based metabolomic analysis of the serum of hypoxic preconditioning mice". Molecular Medicine Reports 16, no. 5 (2017): 6828-6836. https://doi.org/10.3892/mmr.2017.7493
Copy and paste a formatted citation
x
Spandidos Publications style
Liu J, Zhan G, Chen D, Chen J, Yuan ZB, Zhang EL, Gao YX, Xu G, Sun BD, Liao W, Liao W, et al: UPLC‑QTOFMS‑based metabolomic analysis of the serum of hypoxic preconditioning mice. Mol Med Rep 16: 6828-6836, 2017.
APA
Liu, J., Zhan, G., Chen, D., Chen, J., Yuan, Z., Zhang, E. ... Gao, Y. (2017). UPLC‑QTOFMS‑based metabolomic analysis of the serum of hypoxic preconditioning mice. Molecular Medicine Reports, 16, 6828-6836. https://doi.org/10.3892/mmr.2017.7493
MLA
Liu, J., Zhan, G., Chen, D., Chen, J., Yuan, Z., Zhang, E., Gao, Y., Xu, G., Sun, B., Liao, W., Gao, Y."UPLC‑QTOFMS‑based metabolomic analysis of the serum of hypoxic preconditioning mice". Molecular Medicine Reports 16.5 (2017): 6828-6836.
Chicago
Liu, J., Zhan, G., Chen, D., Chen, J., Yuan, Z., Zhang, E., Gao, Y., Xu, G., Sun, B., Liao, W., Gao, Y."UPLC‑QTOFMS‑based metabolomic analysis of the serum of hypoxic preconditioning mice". Molecular Medicine Reports 16, no. 5 (2017): 6828-6836. https://doi.org/10.3892/mmr.2017.7493
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