Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
November-2017 Volume 16 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2017 Volume 16 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

H2O2 induces caveolin‑1 degradation and impaired mitochondrial function in E11 podocytes

  • Authors:
    • Ya‑Hui Chen
    • Wei‑Wen Lin
    • Chin‑San Liu
    • Shih‑Li Su
  • View Affiliations / Copyright

    Affiliations: Vascular and Genomic Center, Changhua Christian Hospital, Changhua 50094, P.R. China, Division of Cardiovascular Center, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung 40705, P.R. China
  • Pages: 7841-7847
    |
    Published online on: September 18, 2017
       https://doi.org/10.3892/mmr.2017.7497
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Increased intercellular reactive oxygen species (ROS) levels are the major cause of podocyte injury with proteinuria. Caveolin‑1 (CAV‑1) is an essential protein component of caveolae. CAV‑1 participates in signal transduction and endocytic trafficking. Recent research has indicated that CAV‑1 regulates oxidative stress‑induced pathways. The present study used hydrogen peroxide (H2O2) at nontoxic concentrations to elevate the level of ROS in E11 podocytes. Treatment with 500 and 1,000 µM H2O2 for 1 h significantly reduced CAV‑1 expression levels. Simultaneously, the treatment significantly reduced the expression of the antioxidant enzymes glutamine‑cysteine ligase catalytic subunit, superoxide dismutase 2 and catalase. To determine the role of CAV‑1 in mediating oxidative stress, E11 podocytes were administered antenapedia‑CAV‑1 (AP‑CAV‑1) peptide for 48 h. The AP‑CAV‑1 treatment enhanced CAV‑1 expression and inhibited cyclophilin A expression, thus reducing ROS‑induced inflammation. Moreover, CAV‑1 protected against H2O2‑induced oxidative stress responses by enhancing the expression of antioxidant enzymes. Furthermore, CAV‑1 attenuated H2O2‑induced changes oxidative phosphorylation, and the expression of optic atrophy 1 and translocase of the inner membrane 23, as well as preserving mitochondrial function. CAV‑1 treatment significantly suppressed apoptosis, as indicated by a higher B‑cell lymphoma 2/BCL2‑associated X protein ratio. Therefore, enhancing the expression of CAV‑1 may be an important therapeutic consideration in treating podocyte injury.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Pavenstädt H, Kriz W and Kretzler M: Cell biology of the glomerular podocyte. Physiol Rev. 83:253–307. 2003. View Article : Google Scholar : PubMed/NCBI

2 

Nagata M: Podocyte injury and its consequences. Kidney Int. 89:1221–1230. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Asanuma K and Mundel P: The role of podocytes in glomerular pathobiology. Clin Exp Nephrol. 7:255–259. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Ronconi E, Mazzinghi B, Sagrinati C, Angelotti ML, Ballerini L, Parente E, Romagnani P, Lazzeri E and Lasagni L: The role of podocyte damage in the pathogenesis of glomerulosclerosis and possible repair mechanisms. G Ital Nefrol. 26:660–669. 2009.PubMed/NCBI

5 

Kashihara N, Haruna Y, Kondeti VK and Kanwar YS: Oxidative stress in diabetic nephropathy. Curr Med Chem. 17:4256–4269. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Bhatti AB and Usman M: Drug Targets for Oxidative Podocyte Injury in Diabetic Nephropathy. Cureus. 7:e3932015.PubMed/NCBI

7 

Mallipattu SK and He JC: The podocyte as a direct target for treatment of glomerular disease? Am J Physiol Renal Physiol. 311:F46–F51. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Panneerselvam M, Patel HH and Roth DM: Caveolins and Heart DiseasesCaveolins and Caveolae: Roles in Signaling and Disease Mechanisms. Jasmin JF, Frank PG and Lisanti MP: Springer US; New York, NY: pp. 145–156. 2012, View Article : Google Scholar

9 

Frank PG, Pavlides S, Cheung MW, Daumer K and Lisanti MP: Role of caveolin-1 in the regulation of lipoprotein metabolism. Am J Physiol Cell Physio. 295:C242–C248. 2008. View Article : Google Scholar

10 

Mercier I, Jasmin JF, Pavlides S, Minetti C, Flomenberg N, Pestell RG, Frank PG, Sotgia F and Lisanti MP: Clinical and translational implications of the caveolin gene family: Lessons from mouse models and human genetic disorders. Lab Invest. 89:614–623. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Trimmer C, Sotgia F, Whitaker-Menezes D, Balliet RM, Eaton G, Martinez-Outschoorn UE, Pavlides S, Howell A, Iozzo RV, Pestell RG, et al: Caveolin-1 and mitochondrial SOD2 (MnSOD) function as tumor suppressors in the stromal microenvironment: A new genetically tractable model for human cancer associated fibroblasts. Cancer Biol Ther. 11:383–394. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Bosch M, Marí M, Herms A, Fernández A, Fajardo A, Kassan A, Giralt A, Colell A, Balgoma D, Barbero E, et al: Caveolin-1 deficiency causes cholesterol-dependent mitochondrial dysfunction and apoptotic susceptibility. Curr Biol. 21:681–686. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Sörensson J, Fierlbeck W, Heider T, Schwarz K, Park DS, Mundel P, Lisanti M and Ballermann BJ: Glomerular endothelial fenestrae in vivo are not formed from caveolae. J Am Soc Nephrol. 13:2639–2647. 2002. View Article : Google Scholar : PubMed/NCBI

14 

Ostalska-Nowicka D, Nowicki M, Zachwieja J, Kasper M and Witt M: The significance of caveolin-1 expression in parietal epithelial cells of Bowman's capsule. Histopathology. 51:611–621. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Ren Z, Liang W, Chen C, Yang H, Singhal PC and Ding G: Angiotensin II induces nephrin dephosphorylation and podocyte injury: Role of caveolin-1. Cell Signal. 24:443–450. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Zhang L, Ren Z, Yang Q and Ding G: Csk regulates angiotensin II-induced podocyte apoptosis. Apoptosis. 21:846–855. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Wan X, Chen Z, Choi WI, Gee HY, Hildebrandt F and Zhou W: Loss of epithelial membrane protein 2 aggravates podocyte injury via upregulation of caveolin-1. J Am Soc Nephrol. 27:1066–1075. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Derossi D, Calvet S, Trembleau A, Brunissen A, Chassaing G and Prochiantz A: Cell internalization of the third helix of the Antennapedia homeodomain is receptor-independent. J Biol Chem. 271:18188–11893. 1996. View Article : Google Scholar : PubMed/NCBI

19 

Bucci M, Gratton JP, Rudic RD, Acevedo L, Roviezzo F, Cirino G and Sessa WC: In vivo delivery of the caveolin-1 scaffolding domain inhibits nitric oxide synthesis and reduces inflammation. Nat Med. 6:1362–1367. 2000. View Article : Google Scholar : PubMed/NCBI

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Nath KA, Fischereder M and Hostetter TH: The role of oxidants in progressive renal injury. Kidney Int Suppl. 45:S111–S115. 1994.PubMed/NCBI

22 

Johnson RJ, Lovett D, Lehrer RI, Couser WG and Klebanoff SJ: Role of oxidants and protease in glomerular injury. Kidney Int. 45:352–359. 1994. View Article : Google Scholar : PubMed/NCBI

23 

Chen F, Barman S, Yu Y, Haigh S, Wang Y, Black SM, Rafikov R, Dou H, Bagi Z, Han W, et al: Caveolin-1 is a negative regulator of NADPH oxidase-derived reactive oxygen species. Free Radic Biol Med. 73:201–213. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Sun LN, Liu XC, Chen XJ, Guan GJ and Liu G: Curcumin attenuates high glucose-induced podocyte apoptosis by regulating functional connections between caveolin-1 phosphorylation and ROS. Acta Pharmacol Sin. 37:645–655. 2016. View Article : Google Scholar : PubMed/NCBI

25 

Volonte D, Liu Z, Musille PM, Stoppani E, Wakabayashi N, Di YP, Lisanti MP, Kensler TW and Galbiati F: Inhibition of nuclear factor-erythroid 2-related factor (Nrf2) by caveolin-1 promotes stress-induced premature senescence. Mol Biol Cell. 24:1852–1862. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Hsieh SR, Hsu CS, Lu CH, Chen WC, Chiu CH and Liou YM: Epigallocatechin-3-gallate-mediated cardioprotection by Akt/GSK-3β/caveolin signalling in H9c2 rat cardiomyoblasts. J Biomed Sci. 20:862013. View Article : Google Scholar : PubMed/NCBI

27 

Mougeolle A, Poussard S, Decossas M, Lamaze C, Lambert O and Dargelos E: Oxidative stress induces caveolin 1 degradation and impairs caveolae functions in skeletal muscle cells. PLoS One. 10:e01226542015. View Article : Google Scholar : PubMed/NCBI

28 

Shang F and Taylor A: Ubiquitin-proteasome pathway and cellular responses to oxidative stress. Free Radic Biol Med. 51:5–16. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Pickering AM and Davies KJ: Degradation of damaged proteins: The main function of the 20S proteasome. Prog Mol Biol Transl Sci. 109:227–248. 2012. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen YH, Lin WW, Liu CS and Su SL: H2O2 induces caveolin‑1 degradation and impaired mitochondrial function in E11 podocytes. Mol Med Rep 16: 7841-7847, 2017.
APA
Chen, Y., Lin, W., Liu, C., & Su, S. (2017). H2O2 induces caveolin‑1 degradation and impaired mitochondrial function in E11 podocytes. Molecular Medicine Reports, 16, 7841-7847. https://doi.org/10.3892/mmr.2017.7497
MLA
Chen, Y., Lin, W., Liu, C., Su, S."H2O2 induces caveolin‑1 degradation and impaired mitochondrial function in E11 podocytes". Molecular Medicine Reports 16.5 (2017): 7841-7847.
Chicago
Chen, Y., Lin, W., Liu, C., Su, S."H2O2 induces caveolin‑1 degradation and impaired mitochondrial function in E11 podocytes". Molecular Medicine Reports 16, no. 5 (2017): 7841-7847. https://doi.org/10.3892/mmr.2017.7497
Copy and paste a formatted citation
x
Spandidos Publications style
Chen YH, Lin WW, Liu CS and Su SL: H2O2 induces caveolin‑1 degradation and impaired mitochondrial function in E11 podocytes. Mol Med Rep 16: 7841-7847, 2017.
APA
Chen, Y., Lin, W., Liu, C., & Su, S. (2017). H2O2 induces caveolin‑1 degradation and impaired mitochondrial function in E11 podocytes. Molecular Medicine Reports, 16, 7841-7847. https://doi.org/10.3892/mmr.2017.7497
MLA
Chen, Y., Lin, W., Liu, C., Su, S."H2O2 induces caveolin‑1 degradation and impaired mitochondrial function in E11 podocytes". Molecular Medicine Reports 16.5 (2017): 7841-7847.
Chicago
Chen, Y., Lin, W., Liu, C., Su, S."H2O2 induces caveolin‑1 degradation and impaired mitochondrial function in E11 podocytes". Molecular Medicine Reports 16, no. 5 (2017): 7841-7847. https://doi.org/10.3892/mmr.2017.7497
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team