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Article

Mitochondrial DNA‑induced inflammatory damage contributes to myocardial ischemia reperfusion injury in rats: Cardioprotective role of epigallocatechin

  • Authors:
    • Chao‑Yi Qin
    • Hong‑Wei Zhang
    • Jun Gu
    • Fei Xu
    • Huai‑Min Liang
    • Kang‑Jun Fan
    • Jia‑Yu Shen
    • Zheng‑Hua Xiao
    • Er‑Yong Zhang
    • Jia Hu
  • View Affiliations / Copyright

    Affiliations: Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Anesthesiology, Chengdu Women and Children's Central Hospital, Chengdu, Sichuan 610000, P.R. China
  • Pages: 7569-7576
    |
    Published online on: September 19, 2017
       https://doi.org/10.3892/mmr.2017.7515
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Abstract

Inflammation serves an important role in the pathogenesis of myocardial ischemia/reperfusion (I/R) injury. Fragments of endogenous damaged‑associated molecular patterns, recently identified as mitochondrial DNA (mtDNA), have been proven to be a potent pro‑inflammatory mediator. Epigallocatechin‑3‑gallate (EGCG) is able to regulate the expression levels of a series of inflammatory cytokines. However, the involvement of endogenous mtDNA in EGCG‑regulated inflammatory activities in the context of myocardial I/R injury remains to be elucidated. The present study was designed to investigate the role of mtDNA in EGCG‑mediated myocardial protection in a rat I/R model. Significant positive correlations between elevated plasma mtDNA copy numbers and the expression levels of tumor necrosis factor (TNF) and interleukins (IL)‑6 and ‑8 were observed in the myocardial tissue following an I/R injury (P<0.05). However, EGCG administered prior to reperfusion was able to effectively downregulate the expression levels of plasma mtDNA, TNF and IL‑6 and ‑8 in the myocardial tissue following an I/R injury (P<0.05). Limited infarct size, reduced severity of myocardial injury and decreased incidence of ventricular arrhythmia were observed in the EGCG‑treated group. However, the beneficial effects of EGCG in preventing myocardial I/R injury may be eliminated by a specific phosphoinositide‑3‑kinase (PI3K) inhibitor. These results suggested that EGCG‑mediated cardioprotective effects may be achieved by inhibiting the release of mtDNA from damaged mitochondria and that this protection was at least in part dependent on the PI3K/RAC‑α serine/threonine‑protein kinase associated signaling pathway.
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1 

Go AS, Mozaffarian D, Roger VL, Benjamin EJ, Berry JD, Blaha MJ, Dai S, Ford ES, Fox CS, Franco S, et al: Heart disease and stroke statistics-2014 update: A report from the American Heart Association. Circulation. 129:e28–e292. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Carrick D and Berry C: Prognostic importance of myocardial infarct characteristics. Eur Heart J Cardiovasc Imaging. 14:313–315. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Minamino T: Cardioprotection from ischemia/reperfusion injury: Basic and translational research. Circ J. 76:1074–1082. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Hausenloy DJ and Yellon DM: Myocardial ischemia-reperfusion injury: A neglected therapeutic target. J Clin Invest. 123:92–100. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Sanada S, Komuro I and Kitakaze M: Pathophysiology of myocardial reperfusion injury: Preconditioning, postconditioning, and translational aspects of protective measures. Am J Physiol Heart Circ Physiol. 301:H1723–H1741. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Zhang Q, Raoof M, Chen Y, Sumi Y, Sursal T, Junger W, Brohi K, Itagaki K and Hauser CJ: Circulating mitochondrial DAMPs cause inflammatory responses to injury. Nature. 464:104–107. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Yue R, Xia X, Jiang J, Yang D, Han Y, Chen X, Cai Y, Li L, Wang WE and Zeng C: Mitochondrial DNA oxidative damage contributes to cardiomyocyte ischemia/reperfusion-injury in rats: Cardioprotective role of lycopene. J Cell Physiol. 230:2128–2141. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Zhang JZ, Liu Z, Liu J, Ren JX and Sun TS: Mitochondrial DNA induces inflammation and increases TLR9/NF-κB expression in lung tissue. Int J Mol Med. 33:817–824. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Williams DL, Ozment-Skelton T and Li C: Modulation of the phosphoinositide 3-kinase signaling pathway alters host response to sepsis, inflammation, and ischemia/reperfusion injury. Shock. 25:432–439. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Hu G, Huang X, Zhang K, Jiang H and Hu X: Anti-inflammatory effect of B-Type natriuretic peptide postconditioning during myocardial ischemia-reperfusion: Involvement of PI3K/Akt signaling pathway. Inflammation. 37:1669–1674. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Wynne AM, Mocanu MM and Yellon DM: Pioglitazone mimics preconditioning in the isolated perfused rat heart: A role for the prosurvival kinases PI3K and P42/44MAPK. J Cardiovasc Pharmacol. 46:817–822. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Boucher M, Pesant S, Falcao S, de Montigny C, Schampaert E, Cardinal R and Rousseau G: Post-ischemic cardioprotection by A2A adenosine receptors: Dependent of phosphatidylinositol 3-kinase pathway. J Cardiovasc Pharmacol. 43:416–422. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Giakoustidis DE, Giakoustidis AE, Iliadis S, Koliakou K, Antoniadis N, Kontos N, Papanikolaou V, Papageorgiou G, Kaldrimidou E and Takoudas D: Attenuation of liver ischemia/reperfusion induced apoptosis by epigallocatechin-3-gallate via down-regulation of NF-kappaB and c-Jun expression. J Surg Res. 159:720–728. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Al-Maghrebi M, Renno WM and Al-Ajmi N: Epigallocatechin-3-gallate inhibits apoptosis and protects testicular seminiferous tubules from ischemia/reperfusion-induced inflammation. Biochem Biophys Res Commun. 420:434–439. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Kim HS, Quon MJ and Kim JA: New insights into the mechanisms of polyphenols beyond antioxidant properties; lessons from the green tea polyphenol, epigallocatechin 3-gallate. Redox Biol. 2:187–195. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Qin C, Liu R, Gu J, Li Y, Qian H, Shi Y and Meng W: Variation of perioperative plasma mitochondrial DNA correlate with peak inflammatory cytokines caused by cardiac surgery with cardiopulmonary bypass. J Cardiothorac Surg. 10:852015. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Miller LE, Hosick PA, Wrieden J, Hoyt E and Quindry JC: Evaluation of arrhythmia scoring systems and exercise-induced cardioprotection. Med Sci Sports Exerc. 44:435–441. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Kim JA, Formoso G, Li Y, Potenza MA, Marasciulo FL, Montagnani M and Quon MJ: Epigallocatechin gallate, a green tea polyphenol, mediates NO-dependent vasodilation using signaling pathways in vascular endothelium requiring reactive oxygen species and Fyn. J Biol Chem. 282:13736–13745. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Elbling L, Weiss RM, Teufelhofer O, Uhl M, Knasmueller S, Schulte-Hermann R, Berger W and Micksche M: Green tea extract and (−)-epigallocatechin-3-gallate, the major tea catechin, exert oxidant but lack antioxidant activities. FASEB J. 19:807–809. 2005.PubMed/NCBI

21 

Potenza MA, Marasciulo FL, Tarquinio M, Tiravanti E, Colantuono G, Federici A, Kim JA, Quon MJ and Montagnani M: EGCG, a green tea polyphenol, improves endothelial function and insulin sensitivity, reduces blood pressure, and protects against myocardial I/R injury in SHR. Am J Physiol Endocrinol Metab. 292:E1378–E1387. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Ai W, Zhang Y, Tang QZ, Yan L, Bian ZY, Liu C, Huang H, Bai X, Yin L and Li H: Silibinin attenuates cardiac hypertrophy and fibrosis through blocking EGFR-dependent signaling. J Cell Biochem. 110:1111–1122. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Cao H, Ye H, Sun Z, Shen X, Song Z, Wu X, He W, Dai C and Yang J: Circulatory mitochondrial DNA is a pro-inflammatory agent in maintenance hemodialysis patients. PLoS One. 9:e1131792014. View Article : Google Scholar : PubMed/NCBI

24 

Pinti M, Cevenini E, Nasi M, De Biasi S, Salvioli S, Monti D, Benatti S, Gibellini L, Cotichini R, Stazi MA, et al: Circulating mitochondrial DNA increases with age and is a familiar trait: Implications for ‘inflamm-aging’. Eur J Immunol. 44:1552–1562. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Zhou R, Yazdi AS, Menu P and Tschopp J: A role for mitochondria in NLRP3 inflammasome activation. Nature. 469:221–225. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Yao X, Carlson D, Sun Y, Ma L, Wolf SE, Minei JP and Zang QS: Mitochondrial ROS induces cardiac inflammation via a pathway through mtDNA damage in a pneumonia-related sepsis model. PLoS One. 10:e01394162015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Qin CY, Zhang HW, Gu J, Xu F, Liang HM, Fan KJ, Shen JY, Xiao ZH, Zhang EY, Hu J, Hu J, et al: Mitochondrial DNA‑induced inflammatory damage contributes to myocardial ischemia reperfusion injury in rats: Cardioprotective role of epigallocatechin. Mol Med Rep 16: 7569-7576, 2017.
APA
Qin, C., Zhang, H., Gu, J., Xu, F., Liang, H., Fan, K. ... Hu, J. (2017). Mitochondrial DNA‑induced inflammatory damage contributes to myocardial ischemia reperfusion injury in rats: Cardioprotective role of epigallocatechin. Molecular Medicine Reports, 16, 7569-7576. https://doi.org/10.3892/mmr.2017.7515
MLA
Qin, C., Zhang, H., Gu, J., Xu, F., Liang, H., Fan, K., Shen, J., Xiao, Z., Zhang, E., Hu, J."Mitochondrial DNA‑induced inflammatory damage contributes to myocardial ischemia reperfusion injury in rats: Cardioprotective role of epigallocatechin". Molecular Medicine Reports 16.5 (2017): 7569-7576.
Chicago
Qin, C., Zhang, H., Gu, J., Xu, F., Liang, H., Fan, K., Shen, J., Xiao, Z., Zhang, E., Hu, J."Mitochondrial DNA‑induced inflammatory damage contributes to myocardial ischemia reperfusion injury in rats: Cardioprotective role of epigallocatechin". Molecular Medicine Reports 16, no. 5 (2017): 7569-7576. https://doi.org/10.3892/mmr.2017.7515
Copy and paste a formatted citation
x
Spandidos Publications style
Qin CY, Zhang HW, Gu J, Xu F, Liang HM, Fan KJ, Shen JY, Xiao ZH, Zhang EY, Hu J, Hu J, et al: Mitochondrial DNA‑induced inflammatory damage contributes to myocardial ischemia reperfusion injury in rats: Cardioprotective role of epigallocatechin. Mol Med Rep 16: 7569-7576, 2017.
APA
Qin, C., Zhang, H., Gu, J., Xu, F., Liang, H., Fan, K. ... Hu, J. (2017). Mitochondrial DNA‑induced inflammatory damage contributes to myocardial ischemia reperfusion injury in rats: Cardioprotective role of epigallocatechin. Molecular Medicine Reports, 16, 7569-7576. https://doi.org/10.3892/mmr.2017.7515
MLA
Qin, C., Zhang, H., Gu, J., Xu, F., Liang, H., Fan, K., Shen, J., Xiao, Z., Zhang, E., Hu, J."Mitochondrial DNA‑induced inflammatory damage contributes to myocardial ischemia reperfusion injury in rats: Cardioprotective role of epigallocatechin". Molecular Medicine Reports 16.5 (2017): 7569-7576.
Chicago
Qin, C., Zhang, H., Gu, J., Xu, F., Liang, H., Fan, K., Shen, J., Xiao, Z., Zhang, E., Hu, J."Mitochondrial DNA‑induced inflammatory damage contributes to myocardial ischemia reperfusion injury in rats: Cardioprotective role of epigallocatechin". Molecular Medicine Reports 16, no. 5 (2017): 7569-7576. https://doi.org/10.3892/mmr.2017.7515
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